HOTAIR/miR-17-5p Axis is Involved in the Propofol-Mediated Cardioprotection Against Ischemia/Reperfusion Injury

Abstract

Background: Propofol (PPF) ameliorates ischemia/reperfusion (I/R) injury in multiple organs by reducing apoptosis and release of pro-inflammatory cytokines. This study aims to explore the mechanism of PPF in attenuating myocardial ischemia-reperfusion injury (MIRI). Materials and Methods: Rat MIRI model was established, and PPF pre-treatment was performed 10 min before I/R. H9c2 cardiomyocytes treated with hypoxia/reoxygenation (H/ R) were used to establish an in vitro model. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to evaluate HOTAIR and miR-17-5p expression levels. Flow cytometry was employed to detect the apoptosis of H9c2 cells. The interaction between HOTAIR and miR-17-5p was determined by bioinformatics analysis, luciferase reporter gene analysis, and RNA immunoprecipitation experiments. STAT3 and p-STAT3 expressions were detected by Western blot. Results: PPF pre-treatment significantly reduced creatine kinase isoenzyme (CK-MB) and serum lactate dehydrogenase (LDH) levels in the serum of the rats with MIRI. PPF pretreatment remarkably up-regulated HOTAIR expression and down-regulated miR-17-5p expression in both in vivo and in vitro models. HOTAIR adsorbed miR-17-5p to repress the expression of miR-17-5p. PPF pre-treatment markedly inhibited cardiomyocyte apoptosis induced by I/R or H/R. HOTAIR knockdown could partially reverse the protective effects of PPF on MIRI. HOTAIR could activate STAT3 signaling via repressing miR-17-5p expression. Conclusion: PPF protects the MIRI by modulating the HOTAIR/miR-17-5p/STAT3 axis.

Genetic and RNA-related molecular markers of trastuzumab-chemotherapy-associated cardiotoxicity in HER2 positive breast cancer: a systematic review

“…Indeed, while on one side when downregulated it increases Trastuzumab cancer cells sensitivity [ 35 ], on the other side, its downregulation reduces the protection of cardio myocytes to ischemia-reperfusion injury. If these two effects may combine to increase CTRCT during Trastuzumab treatment deserves further investigations [ 36 ].…”

Role of sevoflurane in myocardial ischemia-reperfusion injury via the ubiquitin-specific protease 22/lysine-specific demethylase 3A axis

“…Primers were shown in Table 1 . Relative expression of genes was calculated using the 2 −ΔΔCt method [ 23 ].…”

Long Non-coding RNA HOTAIR in Central Nervous System Disorders: New Insights in Pathogenesis, Diagnosis, and Therapeutic Potential

“…HOTAIR also promotes BC progression and metastasis by serving as a sponge for miR-129-5p to upregulate FZD7 expression ( Wu et al, 2021 ). In a rat model of myocardial ischemia-reperfusion injury, HOTAIR upregulation promoted STAT3 expression, positively regulating the HOTAIR/miR-17-5p/STAT3 axis ( Chen et al, 2021 ). In addition, HOTAIR also serves as ceRNA in multiple CNS disorders, such as Parkinson’s disease (PD) ( Zhao et al, 2020 ), MS, and TBI ( Duan et al, 2018 ).…”