Hot Water Extract of <i>Grifola gargal</i> Possesses Anti-inflammatory Activity

Abstract

The anti-inflammatory activity of Grifola gargal was evaluated using an in vitro gut inflammation model composed of RAW264.7 and Caco-2 cells. The active compound was isolated and purified to elucidate its structure by instrumental analysis. A low-molecular-weight fraction in G. gargal prepared using an ultrafiltration membrane (13 000 Da molecular-weight cutoff) suppressed tumor necrosis factor-α production and interleukin-8 mRNA expression in an in vitro gut inflammation model. The low-molecular-weight fraction was subjected to sequential extraction, consisting of reverse-phase extraction using 0-100% ethanol, in 20% steps, and high-performance liquid chromatography to separate and purify the active compound with anti-inflammatory activity. Instrument analyses indicated that adenine was one of the active compounds. It was suggested that adenine in G. gargal had no small effect on the suppression of inflammatory bowel disease.

Grifolamine A, a novel bis-γ-butyrolactone from Grifola frondosa exerted inhibitory effect on α-glucosidase and their binding interaction: Affinity and molecular dynamics simulation

“…The edible mushroom Grifola frondosa , also known as maitake, has been used as a food delicacy and a traditional dietary nutritional supplement for a long history in Asian countries. Over the last few decades, accumulated evidences demonstrated that G. frondosa had outstanding pharmacological properties including hypoglycemic, hypolipidemic, anti-tumor, and immunomodulatory ( Li et al, 2022 ; Mizuno et al, 2017 ; Ouyang et al, 2022 ; Zhang et al, 2022 ). These bioactivities might be attributed to its multiple metabolites including polysaccharides, sterols, ceramides and alkaloids ( Chen et al, 2018 ; Meng et al, 2017 ; Yaoita et al, 2000 ; A.…”