A high burnup commercial spent fuel segment was re-fabricated into a pressurized test train for fuel safety testing in the Irradiated Fuels and Examination Laboratory (IFEL) hot cell facility at Oak Ridge National Laboratory. Descriptions of the in-cell re-fabrication capabilities and assembly of the loss-ofcoolant-accident (LOCA) test train are provided. An in-cell integral LOCA test was performed in the Severe Accident Test Station (SATS) by subjecting the fuel segment to a steam environment, internal pressure, temperatures up to 1200ºC, and water quench. The successful in-cell re-fabrication and integral LOCA demonstration test restores a domestic capability for out-of-pile integral fuel safety testing. In addition, this work establishes the SATS system as a vital tool for evaluation of commercial spent fuel and future accident tolerant fuel (ATF) concepts subject to design basis accident (DBA) and beyond design basis accident (BDBA) scenarios. In-cell Re-fabrication and Loss-of-coolant Accident (LOCA) Testing of High Burnup Commercial Spent Fuel vi