Host Species-Dependent Transmission of Tomato Leaf Curl New Delhi Virus-ES by Bemisia tabaci

Janssen, Dirk; Simón, Almudena; Boulares, Maher; Garcia, Leticia Ruiz

doi:10.3390/plants11030390

Cited by 5 publications

(3 citation statements)

References 51 publications

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“…ToLCNDV-ES isolated from Spain has lower incidence on tomato which is consistent with the reduced transmission efficiency in this plant. A recent report showed that transmission efficiency was significantly higher in zucchini (96%) compared to tomato (2%) by whitefly [ 26 ], so tomato crop can be a potential resource to against ToLCNDV. However, host range extension of ToLCNDV aided by its evolution and the possibility of a different transmission mode are making this virus become a significant threat to cultivations of important crops [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Different Infectivity of Mediterranean and Southern Asian Tomato Leaf Curl New Delhi Virus Isolates in Cucurbit Crops

Lal

et al. 2022

Plants

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Tomato leaf curl New Delhi virus (ToLCNDV) became an alerting virus in Europe from 2017 to 2020 because of its significant damage to Cucurbitaceae cultivation. Until now, just some cucurbit crops including sponge gourd, melon, pumpkin, and cucumber were reported to be resistant to ToLCNDV, but no commercial cultivars are available. In this study, a new isolate of ToLCNDV was identified in Pakistan and analyzed together with ToLCNDV-ES which was previously isolated in Italy. Furthermore, infectious clones of two ToLCNDV isolates were constructed and agroinoculated into different cucurbit crops to verify their infectivity. Results showed that both isolates exhibited severe infection on all tested cucurbit (>70%) except watermelon. Thus, those cultivars may be good candidates in the first step of screening genetic resources for resistance on both Southeast Asian and Mediterranean ToLCNDV isolates. Additional, comparison pathogenicity of different geographical ToLCNDV isolates will be aided to understand viral characterization as such knowledge could facilitate breeding resistance to this virus.

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Section: Discussionmentioning

confidence: 99%

Different Infectivity of Mediterranean and Southern Asian Tomato Leaf Curl New Delhi Virus Isolates in Cucurbit Crops

Lal

et al. 2022

Plants

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“…The virus is transmitted by the whitefly Bemisia tabaci and affects crops from the Solanaceae and Cucurbitaceae families, causing severe economic loses worldwide. This virus is mainly present in Asia, but recently, a recombinant has spread to the Mediterranean basin, affecting mainly zucchini, cucumber and melon and has tomato as a reservoir host (Janssen et al, 2022; Fortes et al, 2016). In Spain, ToLCNDV infection produces severe symptoms in cucurbits, such as curling and distortion of leaves, green and yellow mosaics and other deformations as well as stunting of the plant.…”

Section: Introductionmentioning

confidence: 99%

Exogenous application of dsRNA for the control of viruses in cucurbits

Delgado-Martín

Garcia

Janssen

et al. 2022

Preprint

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The recurrent emergence of virus diseases in intensive horticultural crops requires alternative control strategies. Topical application of dsRNA molecules homologous to pathogens has been proposed as a tool for plant virus control. These dsRNAs induce the silencing mechanism (RNAi) that degrades homologous dsRNAs. Cucumber green mottle mosaic virus (CGMMV) represents a serious threat to cucurbit crops. Since genetic resistance to the virus is not yet available in commercial varieties, we aimed to control this virus by RNAi. For this purpose, we obtained constructions both for expressing dsRNA in bacteria to treat cucumber plants by topical application and for agroinoculation in experiments done in the growth chamber. Besides, greenhouse tests were performed in spring and in summer when plants were challenged with the virus and differences in several parameters were investigated, including severity of symptoms, dry weight, total height, virus accumulation and virus-derived small interfering RNAs (vsiRNAs). Spraying of plants with dsRNA reduced significatively CGMMV symptoms in the plants in growth chamber tests. Agroinfiltration experiments done under identical conditions were also effective in limiting the progress of CGMMV disease. In the greenhouse assay performed in spring, symptoms were significatively reduced in dsRNA-sprayed plants and the development of the plants improved with respect to non-treated plants. Virus titters and vsiRNAs were clearly reduced in dsRNA-treated plants. The effect of protection of the dsRNA was less evident in the greenhouse assay carried out in summer. Besides, we investigated the mobility of long (ds)RNA derived from spraying or agroinfiltrated dsRNA and found that it could be detected in local, close distal and far distal points from the site of application. VsiRNAs were also detected in local and distal points and the differences in accumulation were compared. In parallel, we investigated the capacity of dsRNAs derived from genes of tomato leaf curl New Delhi virus (ToLCNDV), another economically important virus in cucurbits, to limit the disease in zucchini, both by agroinfiltration or direct spraying, but found no protection effect. In view of the results, topical application of dsRNAs is postulated as a promising strategy for CGMMV control in cucumber.

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“…The virus is transmitted by the whitefly Bemisia tabaci and affects crops from the Solanaceae and Cucurbitaceae families, causing severe economic losses worldwide. This virus is mainly present in Asia, but recently, a variant has spread to the Mediterranean basin, affecting mainly zucchini, cucumber, and melon, and has tomato as a reservoir host (Fortes et al, 2016 ; Janssen et al, 2022 ). In Spain, ToLCNDV infection produces severe symptoms in cucurbits, such as curling and distortion of leaves, green and yellow mosaics, and other deformations as well as stunting of the plant.…”

Section: Introductionmentioning

confidence: 99%

Exogenous Application of dsRNA for the Control of Viruses in Cucurbits

Delgado-Martín

Ruiz²,

Janssen³

et al. 2022

Front. Plant Sci.

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The recurrent emergence of viral diseases in intensive horticultural crops requires alternative control strategies. The topical application of double-stranded RNA (dsRNA) molecules homologous to pathogens has been proposed as a tool for virus control in plants. These dsRNAs induce the silencing mechanism, the RNA interference (RNAi), that degrades homologous dsRNAs. Cucumber green mottle mosaic virus (CGMMV) represents a serious threat to cucurbit crops. Since genetic resistance to the virus is not yet available in commercial varieties, we aimed to control this virus by RNAi. For this purpose, we obtained constructions both for expressing dsRNA in bacteria to treat cucumber plants by topical application and for agroinoculation in experiments done in the growth chamber. Besides, greenhouse tests were performed in spring and in summer when plants were challenged with the virus, and differences in several parameters were investigated, including the severity of symptoms, dry weight, total height, virus accumulation, and virus-derived small interfering RNAs (vsiRNAs). Spraying of plants with dsRNA reduced significatively CGMMV symptoms in the plants in growth chamber tests. Agroinfiltration experiments done under identical conditions were also effective in limiting the progress of CGMMV disease. In the greenhouse assay performed in spring, symptoms were significantly reduced in dsRNA-sprayed plants, and the development of the plants improved with respect to non-treated plants. Virus titers and vsiRNAs were clearly reduced in dsRNA-treated plants. The effect of protection of the dsRNA was less evident in the greenhouse assay carried out in the summer. Besides, we investigated the mobility of long (ds)RNA derived from spraying or agroinfiltrated dsRNA and found that it could be detected in local, close distal, and far distal points from the site of application. VsiRNAs were also detected in local and distal points and the differences in accumulation were compared. In parallel, we investigated the capacity of dsRNAs derived from genes of tomato leaf curl New Delhi virus (ToLCNDV), another economically important virus in cucurbits, to limit the disease in zucchini, both by agroinfiltration or by direct spraying, but found no protective effect. In view of the results, the topical application of dsRNAs is postulated as a promising strategy for CGMMV control in the cucumber.

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Host Species-Dependent Transmission of Tomato Leaf Curl New Delhi Virus-ES by Bemisia tabaci

Cited by 5 publications

References 51 publications

Different Infectivity of Mediterranean and Southern Asian Tomato Leaf Curl New Delhi Virus Isolates in Cucurbit Crops

Different Infectivity of Mediterranean and Southern Asian Tomato Leaf Curl New Delhi Virus Isolates in Cucurbit Crops

Exogenous application of dsRNA for the control of viruses in cucurbits

Exogenous Application of dsRNA for the Control of Viruses in Cucurbits

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