Host gut-derived probiotic bacteria promote hypertrophic muscle progression and upregulate growth-related gene expression of slow-growing Malaysian Mahseer Tor tambroides

Asaduzzaman, Md.; Sofia, Ezzah; Shakil, Abrar; Haque, Nayeema Ferdausy; Khan, Mohammed Nurul Absar; Ikeda, Daisuke; Kinoshita, Shigeharu; Abol‐Munafi, Ambok Bolong

doi:10.1016/j.aqrep.2017.12.001

Cited by 45 publications

(48 citation statements)

References 63 publications

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“…The probiotics have shown an improved growth and increased survival of fish infected with Pseudomonas aeruginosa pathogen. The similar results were obtained, when probiotic strain B. subtilis was given to tilapia (Liu et al, ) and Bacillus circulans given to Tor tambroides juveniles (Asaduzzaman et al, ). The results suggest that these probiotics can serve as an effective treatment for fish against various infections with further investigation.…”

Section: Resultssupporting

confidence: 74%

“…Commercially available ‘Hopar grow’ fish food was used as basal diet for fish with the nutrient composition as 30% crude protein, 3% crude fat, 4% crude fibre and 10% moisture during the study. The probiotic‐supplemented feed was prepared as per the protocol given by Ramesh et al () and Asaduzzaman et al (). The bacterial culture was inoculated in MRS broth and incubated at 37°C for 24 hr.…”

Section: Methodsmentioning

confidence: 99%

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Isolation and evaluation of putative probiotic strains from different teleost to prevent Pseudomonas aeruginosa infection in Cyprinus carpio

Chauhan

Singh

2019

Aquac Res

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Probiotics renowned as valuable microbes serve as a potential alternative to control diseases in aquaculture and are considered as an efficient and environment-friendly approach to reduce the use of antibiotics. The present study aims at the isolation of putative probiotic bacteria from the intestinal tract of different fish species from the Doaba region of Punjab, India. In this study, isolated bacterial strains were characterized based on their morphological, biochemical and molecular characterization by 16S rRNA gene sequencing, followed by in vitro evaluation of different selection parameters described in FAO/WHO guidelines. A total of 169 different bacterial strains were isolated from the gastrointestinal tract of 52 different fish species. After in vitro evaluation, out of 169 bacterial strains only five bacteria (S3, S7, BDK2', BDK7 and BDK9) identified as Enterococcus and Bacillus species showed antagonistic activity against the fish pathogen Pseudomonas aeruginosa (MTCC 4 673). These isolates were screened based on their response towards bile tolerance, pH tolerance, adhesion and drug susceptibility to different antibiotic discs. And, the in vivo results indicated improved growth and survival against the infection (P. aeruginosa) after oral administration of the probiotics. The observations of in vitro and in vivo evaluation indicatethat these isolated probiotic strains serve as effective probiotics and can be used as a novel and safe treatment to cure current issues in aquaculture. K E Y W O R D Saquaculture, Cyprinus carpio, Doaba region, Pseudomonas aeruginosa, putative probiotics

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Section: Resultssupporting

confidence: 74%

Section: Methodsmentioning

confidence: 99%

Isolation and evaluation of putative probiotic strains from different teleost to prevent Pseudomonas aeruginosa infection in Cyprinus carpio

Chauhan

Singh

2019

Aquac Res

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“…Real‐time quantitative PCR was performed in a ABI Prism 7300 Sequence Detection System (Applied Biosystem) using SsoAdvanced™ Universal SYBR ® Green Supermix (Bio‐Rad). The amplification was performed as described by Asaduzzaman, Sofia, et al (). The transcript quantities were represented as the relative expression levels of target genes to those of reference genes.…”

Section: Methodsmentioning

confidence: 99%

“…However, the major challenge of T. tambroides culture in captive condition is the slow growth rate during their grow‐out farming (Lee et al, ). Therefore, extensive studies have been conducting for years to improve the growth performance of this fish species mostly focusing on feed development (Asaduzzaman, Iehata, et al, ; Asaduzzaman et al, , ; Asaduzzaman, Sofia, et al, ; Ismail, Salleh, & Ramezani, ; Kamarudin, Ramezani‐Fard, Saad, & Harmin, ; Ng, Abdullah, & De Silva, ; Ng & Andin, ; Ramezani‐Fard, Kamarudin, Harmin, & Saad, ; Ramezani‐Fard, Kamarudin, Saad, Harmin, & Meng, ). However, little emphasis has been given to investigate the possible effects resulting from the dietary supplementation of any prebiotic to enhance growth performance of this slow‐growing T. tambroides .…”

Section: Introductionmentioning

confidence: 99%

Sodium alginate supplementation modulates gut microbiota, health parameters, growth performance and growth‐related gene expression in Malaysian MahseerTor tambroides

et al. 2019

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This study investigated the effects of sodium alginate supplementation on gut microbiota composition, health parameters, growth performances and growth‐related gene expression of Malaysian mahseer. Five test diets were formulated by supplementing 0%, 0.1%, 0.2%, 0.4% and 0.8% sodium alginate. Triplicate groups of juvenile Tor tambroides (2.19 ± 0.05 g) were stocked in 15 aquaria (20 individuals per aquarium) and fed at 3.0% body weight per day for 60 days. PCoA and UPGMA analysis showed that gut bacterial community were more convergence in higher sodium alginate‐supplemented diets. The percentage of Porphyromonadaceae, Bacteroides, Plesiomonas and Shewanella were substantially higher and Aeromonas, Entomoplasmatales and Prevotellaceae were drastically lower in higher sodium alginate (0.2%–0.8%) diets. Sodium alginate supplementation (≥0.2%) significantly improved the haematocrit value and respiratory burst activity of T. tambroides. Growth performances and feed utilization were significantly higher in 0.2%–0.4% sodium alginate‐supplemented diets. The increased growth rate of T. tambroides was governed by both hyperplastic and hypertrophic muscle growth. Real‐time PCR data demonstrated that most of the growth‐related genes were significantly upregulated in 0.2%–0.4% sodium alginate‐supplemented diet. Finally, it can be concluded that sodium alginate should be supplemented at 2 g/kg in practical fish feed formulation.

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“…There were many studies on the different feed formulations and feed additives that improved the growth rate of T. tambroides (Ishak, Kamarudin, Ramezani‐Fard, & Yusof, ; Kamarudin, Ramezani‐Fard, Saad, & Harmin, ; Misieng, Kamarudin, & Musa, ; Ng & Andin, ; Ng et al., ; Ramezani‐Fard, Kamarudin, Saad, Harmin, & Goh, ). There were also studies on effects of host gut‐derived probiotic bacteria to T. tambroides where the probiotic improved nutrients utilization and metabolism, adjusting gut microbiota balance and enhanced growth by promoting muscle fiber hypertrophy (Asaduzzaman, Iehata, et al., ; Asaduzzaman, Sofia, et al., ). Nevertheless, there is no report on the phylogenetic and functional characterization of gut microbiota of T. tambroides .…”

Section: Introductionmentioning

confidence: 99%

Comparative study of gut microbiota in wild and captive Malaysian Mahseer (Tor tambroides)

et al. 2018

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Aims The aim of this study was to identify and compare the gut microbial community of wild and captive Tor tambroides through 16S rDNA metagenetic sequencing followed by functions prediction. Methods and results The library of 16S rDNA V3‐V4 hypervariable regions of gut microbiota was amplified and sequenced using Illumina MiSeq. The sequencing data were analyzed using Quantitative Insights into Microbial Ecology ( QIIME ) pipeline and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States ( PICRUS t). The most abundant bacterial phyla in both wild and captive T. tambroides were Firmicutes, Proteobacteria, Fusobacteria and Bacteroidetes. Cetobacterium spp., Peptostreptococcaceae family, Bacteroides spp., Phosphate solubilizing bacteria PSB ‐M‐3, and Vibrio spp. were five most abundant OTU in wild T. tambroides as compared to Cetobacterium spp., Citrobacter spp., Aeromonadaceae family, Peptostreptococcaceae family and Turicibacter spp. in captive T. tambroides . Conclusion In this study, the specimens of the wild T. tambroides contain more diverse gut microbiota than of the captive ones. The results suggested that Cetobacterium spp. is one of the core microbiota in guts of T. tambroides . Besides, high abundant Bacteroides spp., Citrobacter spp., Turicibacter spp., and Bacillus spp. may provide important functions in T. tambroides guts. Significance and impact of the study The results of this study provide significant information of T. tambroides gut microbiota for further understanding of their physiological functions including growth and disease resistance.

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Host gut-derived probiotic bacteria promote hypertrophic muscle progression and upregulate growth-related gene expression of slow-growing Malaysian Mahseer Tor tambroides

Cited by 45 publications

References 63 publications

Isolation and evaluation of putative probiotic strains from different teleost to prevent Pseudomonas aeruginosa infection in Cyprinus carpio

Isolation and evaluation of putative probiotic strains from different teleost to prevent Pseudomonas aeruginosa infection in Cyprinus carpio

Sodium alginate supplementation modulates gut microbiota, health parameters, growth performance and growth‐related gene expression in Malaysian MahseerTor tambroides

Comparative study of gut microbiota in wild and captive Malaysian Mahseer (Tor tambroides)

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