Host–Guest Interaction of Chaperonin GroEL and Water‐Soluble CdTe Quantum Dots and its Size‐Selective Inclusion

Dong, Chaoqing; Shao, Liwen; Guo, Jiacheng; Ren, Jicun

doi:10.1002/cphc.200800398

Cited by 7 publications

(7 citation statements)

References 82 publications

(47 reference statements)

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“…The data were fitted using a non‐linear fitting procedure according to equation (3). Thus :

normaly = \frac{(\frac{1}{K_{a}} + {normalC}_{ssDNA} + {normalC}_{dsDNA})}{2 C_{ssDNA}} - {[{((), \frac{\frac{1}{K_{a}} + {normalC}_{ssDNA} + {normalC}_{dsDNA}}{2 {normalC}_{ssDNA}})}^{2} - \frac{C_{dsDNA}}{C_{ssDNA}}]}^{\frac{1}{2}}

…”

Section: Resultsmentioning

confidence: 99%

“…The data were fitted using a non-linear fitting procedure according to equation (3). Thus (33,38): where C ssDNA and C dsDNA are the initial concentrations of ssDNA and dsDNA, respectively, and y is the binding ratio of ssDNA, which is determined from each titration point in the FCS measurements and averaged over three parallel experiments (39). As shown in Fig.…”

Section: Association Constants For Triplex Dna With Different Length mentioning

confidence: 99%

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Studies on the formation and stability of triplex DNA using fluorescence correlation spectroscopy

Huang

Ren

2015

Luminescence

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Triplex DNA has become one of the most useful recognition motifs in the design of new molecular biology tools, therapeutic agents and sophisticated DNA-based nanomaterials because of its direct recognition of natural double-stranded DNA. In this paper, we developed a sensitive and microscale method to study the formation and stability characterization of triplex DNA using fluorescence correlation spectroscopy (FCS). The principle of this method is mainly based on the excellent capacity of FCS for sensitively distinguishing between free single-strand DNA (ssDNA) fluorescent probes and fluorescent probe-double-strand DNA (dsDNA) hybridized complexes. First, we systematically investigated the experimental conditions of triplex DNA formation. Then, we evaluated the equilibrium association constants (K(a)) under different ssDNA probe lengths, composition and pH. Finally, we used FCS to measure the hybridization fraction of a 20-mer perfectly matched ssDNA probe and three single-base mismatched ssDNA probes with 146-mer dsDNA. Our data illustrated that FCS is a useful tool for the direct determination of the thermodynamic parameters of triplex DNA formation and discrimination of a single-base mismatch of triplex DNA without denaturation. Compared with current methods, our method is characterized by high sensitivity, good universality and small sample and reagent requirements. More importantly, our method has the potential to become a platform for triplex DNA research in vitro.

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“…The data were fitted using a non‐linear fitting procedure according to equation (3). Thus :

normaly = \frac{(\frac{1}{K_{a}} + {normalC}_{ssDNA} + {normalC}_{dsDNA})}{2 C_{ssDNA}} - {[{((), \frac{\frac{1}{K_{a}} + {normalC}_{ssDNA} + {normalC}_{dsDNA}}{2 {normalC}_{ssDNA}})}^{2} - \frac{C_{dsDNA}}{C_{ssDNA}}]}^{\frac{1}{2}}

…”

Section: Resultsmentioning

confidence: 99%

Section: Association Constants For Triplex Dna With Different Length mentioning

confidence: 99%

Studies on the formation and stability of triplex DNA using fluorescence correlation spectroscopy

Huang

Ren

2015

Luminescence

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“…In a similar study, Weng et al evaluated the feasibility of application of CdTe QDs conjugated with Ulex europaeus 1 and antivon Willebrand factor using CE-LIF [80]. Similar reports were also found in several research groups [80][81][82][83][84][85][86]. Recently, Wang et al systematically investigated the conjugation of QDs with certain biomolecules using CE-LIF and FCS [87].…”

Section: Qd-protein Conjugatesmentioning

confidence: 85%

Characterization and separation of semiconductor quantum dots and their conjugates by capillary electrophoresis

2014

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Semiconductor quantum dots (QDs) are very important luminescent nanomaterials with a wide range of potential applications. Currently, QDs as labeling probes are broadly used in bioassays, including immunoassay, DNA hybridization, and bioimaging, due to their excellent physical and chemical properties, such as broad excitation spectra, narrow and size-dependent emission profiles, long fluorescence life time, and good photostability. The characterization of QDs and their conjugates is crucial for their wide bioapplications. CE has become a powerful tool for the separation and characterization of QDs and their conjugates. In this review, some CE separation models of QDs are first introduced, mainly including CZE, CGE, MEKC, and ITP. And then, some key applications, such as the measurements of size, surface charge, and concentration of QDs and the characterization of QDs conjugates (e.g. QD-protein, QD-DNA, QD-small molecule), are also described. Finally, future perspectives are discussed.

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“…It was reported that CdTe QDs exhibited adsorption to some biomacromolecules such as bovine serum albumin and antibodies [44,45]. Therefore, the potential mechanism of CdTe QDs' enhancing effects may lie at the following issues.…”

Section: Discussionmentioning

confidence: 99%

“…Fluorescence correlation spectroscopy (FCS) measurements were performed with a home-built FCS system, which was based on an inverted Olympus IX 71 microscope (Japan) [44]. The illumination source was an argon laser with 488 nm laser line (ILT Technology, Shanghai, China).…”

Section: Fluorescence Correlation Spectroscopymentioning

confidence: 99%

Quantum dots trigger hot-start effects for pfu-based polymerase chain reaction

Sang

Yang

Wang

et al. 2013

Journal of Experimental Nanoscience

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Hot-start (HS) effects were investigated in pfu-based polymerase chain reaction (PCR), when water-soluble CdTe quantum dots (QDs) were introduced in the PCR system. The HS effects were demonstrated by the higher amplicon yields and excellent suppression of non-specific amplification after pre-incubation of PCR mix with QDs between 35 C and 56 C. DNA targets were well amplified even after PCR mixture was pre-incubated 1 h at 50 C. Importantly, the effects of QDs nanoparticles could be reversed by increasing the pfu polymerase concentration, suggesting that there was an interaction between QDs and pfu DNA polymerase. Moreover, control experiment indicated that HS effect is not primarily due to the reduced pfu polymerase concentration resulted from the above interaction. Fluorescence correlation spectroscopy (FCS), a single molecule detection method, was used to investigate the possible mechanism of HS PCR with QDs. Preliminary FCS results suggested that CdTe QDs may directly interact with pfu DNA polymerase, rather than other components in the PCR system. Furthermore, results demonstrated that the interaction between QDs and pfu resulted in a reduction in pfu polymerase concentration. This study provided a good start to investigate potential implications of QDs in other key molecular biology techniques.

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Host–Guest Interaction of Chaperonin GroEL and Water‐Soluble CdTe Quantum Dots and its Size‐Selective Inclusion

Cited by 7 publications

References 82 publications

Studies on the formation and stability of triplex DNA using fluorescence correlation spectroscopy

Studies on the formation and stability of triplex DNA using fluorescence correlation spectroscopy

Characterization and separation of semiconductor quantum dots and their conjugates by capillary electrophoresis

Quantum dots trigger hot-start effects for pfu-based polymerase chain reaction

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