Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion

Granadero, Daniel; Bordello, Jorge; Pérez-Alvite, María Jesús; Novo, Mercedes; Al‐Soufi, Wajih

doi:10.3390/ijms11010173

Cited by 94 publications

(79 citation statements)

References 45 publications

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“…S21). When looking at the binding constant of mono- and bivalent Ad with multivalent β-CD hosts in general51525354, a difference of at least a factor 200 is found. The binding constant of bis-adamantane (e.g.…”

Section: Resultsmentioning

confidence: 99%

Obtaining control of cell surface functionalizations via Pre-targeting and Supramolecular host guest interactions

Rood

Spa

Welling

et al. 2017

Sci Rep

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The use of mammalian cells for therapeutic applications is finding its way into modern medicine. However, modification or “training” of cells to make them suitable for a specific application remains complex. By envisioning a chemical toolbox that enables specific, but straight-forward and generic cellular functionalization, we investigated how membrane-receptor (pre)targeting could be combined with supramolecular host-guest interactions based on β-cyclodextrin (CD) and adamantane (Ad). The feasibility of this approach was studied in cells with membranous overexpression of the chemokine receptor 4 (CXCR4). By combining specific targeting of CXCR4, using an adamantane (Ad)-functionalized Ac-TZ14011 peptide (guest; KD = 56 nM), with multivalent host molecules that entailed fluorescent β-CD-Poly(isobutylene-alt-maleic-anhydride)-polymers with different fluorescent colors and number of functionalities, host-guest cell-surface modifications could be studied in detail. A second set of Ad-functionalized entities enabled introduction of additional surface functionalities. In addition, the attraction between CD and Ad could be used to drive cell-cell interactions. Combined we have shown that supramolecular interactions, that are based on specific targeting of an overexpressed membrane-receptor, allow specific and stable, yet reversible, surface functionalization of viable cells and how this approach can be used to influence the interaction between cells and their surroundings.

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Section: Resultsmentioning

confidence: 99%

Obtaining control of cell surface functionalizations via Pre-targeting and Supramolecular host guest interactions

Rood

Spa

Welling

et al. 2017

Sci Rep

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“…The necessary theory has been described in more detail elsewhere and only a brief overview will be given here [3,4,18].…”

Section: Theorymentioning

confidence: 99%

“…The setup used for the FCS measurements has been described elsewhere [18]. Analysentechnik, Tübingen, Germany) in front of the beamsplitter discriminated fluorescence from scattered light.…”

Section: Equipmentmentioning

confidence: 99%

Exchange-dynamics of a neutral hydrophobic dye in micellar solutions studied by Fluorescence Correlation Spectroscopy

Bordello

Novo

Al‐Soufi

2010

Journal of Colloid and Interface Science

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“…4,13 The fluorescent domain of the drug allows for easy detection. The drug also offers great flexibility: ongoing research in our laboratory suggests that the monovalent Ad derivative may be chemically modified to synthesize multivalent derivatives.…”

Section: Introductionmentioning

confidence: 99%

Experimental Studies and Modeling of Drug Release from a Tunable Affinity-Based Drug Delivery Platform

Thatiparti

Saidel

et al. 2011

Ann Biomed Eng

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An affinity-based drug delivery platform for controlling drug release is analyzed by a combination of experimental studies and mathematical modeling. This platform has the ability to form selective interactions between a therapeutic agent and host matrix that yields advantages over systems that employ nonselective methods. The incorporation of molecular interactions in drug delivery can increase the therapeutic lifetime of drug delivery implants and limit the need for multiple implants in treatment of chronic illnesses. To analyze this complex system for rational design of drug delivery implants, we developed a mechanistic mathematical model to quantify the molecular events and processes. With a β-cyclodextrin hydrogel host matrix, defined release rates were obtained using a fluorescent model drug. The key processes were the complexation between the drug and cyclodextrin and diffusion of the drug in the hydrogel. Optimal estimates of the model parameters were obtained by minimizing the difference between model simulation and experimentally measured drug release kinetics. Model simulations could predict the drug release dynamics under a wide range of experimental conditions.

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Host-Guest Complexation Studied by Fluorescence Correlation Spectroscopy: Adamantane–Cyclodextrin Inclusion

Cited by 94 publications

References 45 publications

Obtaining control of cell surface functionalizations via Pre-targeting and Supramolecular host guest interactions

Obtaining control of cell surface functionalizations via Pre-targeting and Supramolecular host guest interactions

Exchange-dynamics of a neutral hydrophobic dye in micellar solutions studied by Fluorescence Correlation Spectroscopy

Experimental Studies and Modeling of Drug Release from a Tunable Affinity-Based Drug Delivery Platform

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