Exchange-dynamics of a neutral hydrophobic dye in micellar solutions studied by Fluorescence Correlation Spectroscopy

Hydrodynamic size characterization of a self-emulsifying lipid pharmaceutical excipient by Taylor dispersion analysis with fluorescent detection

“…The advantage of FCS is the possibility to work in dilute solutions due to the high sensitivity of the technique. FCS was successfully applied for the measurements of micelle diffusion coefficients and consequently their hydrodynamic radii (Al-Soufi et al, 2012;Bordello et al, 2010;Gapi nski et al, 2010;Novo et al, 2007;Piñeiro et al, 2013Piñeiro et al, , 2015, but its application for larger sized droplets such as microemulsions (Burnett et al, 2004;Pal et al, 2011;Richacek-ka et al, 1990) and emulsions (Khan et al, 2016) is still limited probably due to the difficulty to measure particle sizes larger than 1/10th of the observation volume's size (Khan et al, 2016).…”

Single‐Molecule Approach to DNA Minor‐Groove Association Dynamics

“…Details of the synthesis of the BBA-OG can also be found in the Supporting Information. Experimental setup and conditions, as well as the details of the data analysis for the FCS measurements have been published elsewhere [18,22] and are specified for these experiments in the Supporting Information. The uncertainties given in Table 1 represent one standard deviation as obtained by the fits.…”

“…The large amplitude A R of this term is because of the large increase in the brightness of bound BBA-OG. [18] The diffusion and relaxation terms show the expected concentration dependence and are independent of the irradiance. [18,19] To reduce parameter correlation, the FCS titration series were analyzed by global target fits [14,15] with a common dynamic-equilibrium model of the diffusion, triplet, and relaxation terms (Figure 1 and Figure S1-S4).…”

Fluorophore exchange kinetics in block copolymer micelles with varying solvent–fluorophore and solvent–polymer interactions

“…One mode of guest exchange involves diffusive processes, 28 in which the guest molecules are expelled from a host, diffuse through the solvent, and reinsert into a guest, and the exchange dynamics are dominated by the rate at which guest molecules exit the host. 29 In addition, there are collision-based processes which involve fission or fusion. 30–32 Many factors affect the prevalence of these exchange mechanisms as well as the partition coefficient for the guest molecule, including strength of interactions between the guest and micelle core (modulated by the block length in the hydrophobic block of block copolymer systems 33 ), and strength of interactions between the guest molecule and the bulk solvent.…”