Host genetic variations in glutathione-S-transferases, superoxide dismutases and catalase genes influence susceptibility to malaria infection in an Indian population

Abstract

Antioxidant enzymes can contribute to disease susceptibility or determine response to therapy in individuals with malaria. Genetic variations due to polymorphisms in host genes encoding antioxidant enzymes such as glutathione S-transferases-theta, mu, pi (GSTT, GSTM, GSTP), superoxide dismutases (SOD) and catalase (CAT), may therefore, influence inter-individual response to malaria pathology and propensity of infection caused by Plasmodium vivax (Pv) and Plasmodium falciparum (Pf). Therefore, using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing, we investigated the association of deletions of GSTT1 and GSTM1, single nucleotide polymorphisms (SNPs) of GSTP1 (rs1695), SOD1 (rs2234694), SOD2 (rs4880, rs1141718), SOD3 (rs2536512) and CAT (rs1001179) in individuals infected with Pf (n = 100) and Pv (n = 100) against healthy controls (n = 150). Our data suggest a significant role for GSTM1 deletions in complicated Pv (p = 0.0007) malaria with ODDs ratio 3.8 [with 95 % confidence interval (CI) 1.9-7.4]. The results also indicated that polymorphisms present in GSTP1, SOD1 and CAT genes may be associated with malaria susceptibility (p < 0.05), whereas SOD3 polymorphism may play a role in malarial resistance (p < 0.05). In addition, we observed significant SNP-SNP interactions with synergistic genetic effects in SOD2, SOD3 and CAT genes for Pv and in SOD2 and SOD3 genes for Pf. In conclusion, our results provide convincing evidence for a relationship between polymorphisms in host antioxidant enzymes and susceptibility to malaria infection.

Teratozoospermia and karyopathological abnormalities of blood cells in human granulocytic anaplasmosis depending on polymorphism of GSTM1 gene of glutathion-S-transferase

“…Ранее нами показано, что у больных такими клещевыми инфекциями, как клещевой энцефалит и иксодовый клещевой боррелиоз, в случае носительства делеции гена GSTM1(0/0) фермента глутатион-Sтрансферазы наблюдаются тератозооспермия и повышенная частота встречаемости цитогенетических аберраций в соматических клетках [4][5][6]. Однако в литературе мы не нашли исследований, посвященных изучению способности инфекции, вызывающей ГАЧ, индуцировать тератозооспермию или/и цитогенетические нарушения у больных людей.…”

“…ального времени с использованием амплификатора Терцик (Россия). ДНК для ПЦР выделяли из проб венозной крови [4,6]. Продукты амплификации и рестрикции ампликонов разделяли в горизонтальном 3% агарозном геле с применением камеры для горизонтального электрофореза ЕС 12-13 («Биоком», Москва, Россия).…”

Categorical complexities of Plasmodium falciparum malaria in individuals is associated with genetic variations in ADORA2A and GRK5 genes

“…It has also been reported that peptides disrupting the interaction of Gas and b2AR block erythrocyte invasion by Pf parasites in vitro (Harrison et al, 2003). Genetic screening of individuals for the factors affecting erythrocyte phase of infection Saadi et al, 2013;Fernandes et al, 2015) in endemic areas, may provide new insight into the possible mechanisms that may explain the varied susceptibility of individuals to malarial infection. Reports that are available suggest that adenosine receptors play a major role in cellular functions by coupling to G proteins, although several effects independent of G-proteins have also been reported (Fredholm et al, 2007).…”

Association of Catalase and Glutathione Peroxidase 1 Polymorphisms with Chronic Hepatitis C Outcome

“…Studies have shown that the T allele has been associated with increased levels of CAT when compared to the C allele (Forsberg et al, ; Galecki et al, ; Hebert‐Schuster et al, ; Kodydková et al, ). Additionally, this polymorphism has been associated with many pathological conditions, such as: HCC (Ezzikouri et al, ), breast cancer (Ahn et al, ), asthma (Mak et al, ), rheumatoid arthritis (Bohanec Grabar et al, ), and susceptibility to malaria (Fernandes et al, ).…”