Host defense peptide IDR-1002 associated with ciprofloxacin as a new antimicrobial and immunomodulatory strategy for dental pulp revascularization therapy

Sousa, Maurício Gonçalves C.; Xavier, Patrícia Diniz; Cantuária, Ana Paula de Castro; Porcino, Rayssa A.; Almeida, Jeeser Alves de; Franco, Octávio Luiz; Rezende, Taia Maria Berto

doi:10.1016/j.micpath.2020.104634

Cited by 15 publications

(14 citation statements)

References 60 publications

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“…Although IDR-1002 was minimally released from PVA/CS nanofibers, it had anti-biofilm and/or antimicrobial activity, presumably due to direct contact. Moreover, we previously demonstrated that the association of IDR-1002 with ciprofloxacin improves its antimicrobial potential [ 21 ]. The results of the anti-biofilm activity of nanostructured molecules were close to what was found with the free molecules ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…For this, 1 × 10 12 CFU mL −1 of E. faecalis and 2.9 × 10 8 CFU mL −1 S. aureus were used. The antibacterial bioassays were carried out in LB media, with 5 × 10 4 CFU per well [ 36 ]. Nanofibers were cut in a circular shape (approximately 6 mm of diameter and 1 mg) and sterilized overnight in UV light.…”

Section: Methodsmentioning

confidence: 99%

“…HDPs are promising alternatives for endodontic treatment [ 20 ]. We recently demonstrated that IDR-1002 and ciprofloxacin demonstrated an in vitro synergistic antimicrobial effect against S. aureus and E. faecalis [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Antibiofilm and immunomodulatory resorbable nanofibrous filing for dental pulp regenerative procedures

Sousa

Almeida

Mota

et al. 2022

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Antibiofilm and immunomodulatory resorbable nanofibrous filing for dental pulp regenerative procedures

Sousa

Almeida

Mota

et al. 2022

Bioactive Materials

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“…Checkerboard assay. The synergistic activities of the CFTR modulators combined with tobramycin (TOB), ciprofloxacin (CIP), colistin (CST), PMB, meropenem (MER) and azithromycin (AZM) for P. aeruginosa and amoxicillin (AMX), teicoplanin (TEC), linezolid (LZD), vancomycin (VAN) and AZM for S. aureus, were determined by the checkerboard method in cation-adjusted Mueller-Hinton broth [33], as detailed in the Supplementary Methods.…”

Section: Methodsmentioning

confidence: 99%

Linking CFTR modulators to opportunistic bacterial infections in cystic fibrosis

Cigana

Giannella

Colavolpe

et al. 2022

Preprint

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Cystic fibrosis transmembrane conductance regulator (CFTR) modulators improve clinical outcomes with variable efficacy in patients with cystic fibrosis (CF). However, changes produced by bacterial persistence and adaptation in addition to antibiotic regimens could influence CFTR modulator efficacy and vice versa and hence clinical outcomes. We first evaluated the effects of ivacaftor (IVA), lumacaftor (LUM), tezacaftor, elexacaftor and elexacaftor/tezacaftor/ivacaftor (ETI), alone or combined with antibiotics, on sequential Staphylococcus aureus and Pseudomonas aeruginosa CF isolates. IVA and ETI showed the most potent direct antimicrobial activity against S. aureus, while P. aeruginosa was not affected. Additive effects or synergies were observed between the CFTR modulators and antibiotics against both S. aureus and P. aeruginosa, independently of the stage of colonization. IVA and LUM were the most effective in potentiating antibiotic activity against S. aureus, while IVA and ETI enhanced mainly polymyxins activity against P. aeruginosa. Next, we evaluated the effect of P. aeruginosa pneumonia on the pharmacokinetics of IVA in mice. The time-concentration curves of IVA and its metabolites in plasma, lung and epithelial lining fluid were influenced by P. aeruginosa infection. The area under the concentration-time curve showed that airway exposure to IVA was greater in infected than non-infected mice. These results suggest that CFTR modulators can have direct antimicrobial properties and/or enhance antibiotic activity against S. aureus and P. aeruginosa. Furthermore, bacterial infection impacts the IVA concentration and airway exposure, potentially affecting its efficacy. Our findings suggest optimizing host- and pathogen-directed drug regimens to improve efficacy for personalized treatment.

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“…IDR-1002 is a synthetic peptide which has anti-inflammatory and antibacterial properties against E. faecalis. It is also a potential synergistic agent of ciprofloxacin in regenerative therapies (Sousa et al, 2020 • Serve as tumor suppressor gene in oral squamous cell carcinoma hBD-1 hBD-2 HNP1 HNP2 HNP3…”

Section: Artificial Antimicrobial Peptidesmentioning

confidence: 99%

The multifaceted roles of antimicrobial peptides in oral diseases

Niu

Yin

Mei

et al. 2021

Molecular Oral Microbiology

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Antimicrobial peptides are naturally occurring protein molecules with antibacterial, antiviral and/or antifungal activity. Some antimicrobial peptides kill microorganisms through direct binding with negatively charged microbial surfaces. This action disrupts the cytoplasmic membrane and leads to the leakage of the cytoplasm. In addition, they are involved in the innate immune response. Antimicrobial peptides play an important role in oral health, as natural antimicrobial peptides are the first line of host defence in response to microbial infection. The level of natural antimicrobial peptides increases during severe disease conditions and play a role in promoting the healing of oral tissues. However, they are insufficient for eliminating pathogenic micro-organisms. The variability of the oral environment can markedly reduce the effect of natural antimicrobial peptides. Thus, researchers are developing synthetic antimicrobial peptides with promising stability and biocompatibility. Synthetic antimicrobial peptides are a potential alternative to traditional antimicrobial therapy.Pertinent to oral diseases, the deregulation of antimicrobial peptides is involved in the pathogenesis of dental caries, periodontal disease, mucosal disease and oral cancer, where they can kill pathogenic microorganisms, promote tissue healing, serve as biomarkers and inhibit tumor cells. This narrative review provides an overview of the multifaceted roles of antimicrobial peptides in oral diseases.

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Host defense peptide IDR-1002 associated with ciprofloxacin as a new antimicrobial and immunomodulatory strategy for dental pulp revascularization therapy

Cited by 15 publications

References 60 publications

Antibiofilm and immunomodulatory resorbable nanofibrous filing for dental pulp regenerative procedures

Antibiofilm and immunomodulatory resorbable nanofibrous filing for dental pulp regenerative procedures

Linking CFTR modulators to opportunistic bacterial infections in cystic fibrosis

The multifaceted roles of antimicrobial peptides in oral diseases

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