Host cell tropism, genome characterization, and evolutionary features of OaPV4, a novel Deltapapillomavirus identified in sheep fibropapilloma

Tore, Gessica; Cacciotto, Carla; Anfossi, Antonio Giovanni; Dore, Gian Mario; Antuofermo, Elisabetta; Scagliarini, Alessandra; Burrai, Giovanni Pietro; Pau, Salvatore; Zedda, Maria Teresa; Masala, Gerolamo; Pittau, Marco; Alberti, Alberto

doi:10.1016/j.vetmic.2017.04.024

Cited by 23 publications

(35 citation statements)

References 29 publications

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“…PCR analysis failed to amplify DNA using primers for ovine OrfV in pathological tissue samples. Furthermore, neither DNA fragments from three ovine δPVs (OaPV-1, -2 and -4), known to be involved in cutaneous fibropapillomas of sheep 28 nor OaPV-3, a dyoκPV associated to ovine squamous cell carcinoma 29 were amplified. DNA was amplified as expected from positive control tissues using these primers.…”

Section: Resultsmentioning

confidence: 96%

Oral fibropapillomatosis and epidermal hyperplasia of the lip in newborn lambs associated with bovine Deltapapillomavirus

Roperto

Russo

Corrado

et al. 2018

Sci Rep

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Congenital fibropapillomatosis of the gingiva and oral mucosa and epidermal hyperplasia of the lip are described, for the first time, in two newborn lambs. Expression of the E5 oncoprotein of bovine deltapapillomavirus types 2 (BPV-2) and -13 (BPV-13) was detected in both fibropapillomas and the hyperplastic epidermal cells suggesting the BPV infection was the cause of the proliferative lesions. No DNA sequences of BPV-1 and BPV-14 were detected. Both BPV-2 and BPV-13 DNA were also amplified from peripheral blood mononuclear cells (PBMCs) of the newborn lambs’ dams. The concordance between BPV genotypes detected in the blood of dam and the oral and skin pathological samples of their offspring suggests that a vertical hematogeneous transmission was most likely source of BPV infection. Immunoblotting revealed the presence of E5 dimers allowing the viral protein to be biologically active. E5 dimers bind and activate the platelet derived growth factor β receptor (PDGFβR), a major molecular mechanism contributing to disease. The detection of E5 protein within the proliferating cells therefore adds further evidence that the BPV infection was the cause of the proliferative lesions seen in these lambs. This is the first evidence of vertical transmission of BPVs in sheep resulting in a clinical disease.

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Section: Resultsmentioning

confidence: 96%

Oral fibropapillomatosis and epidermal hyperplasia of the lip in newborn lambs associated with bovine Deltapapillomavirus

Roperto

Russo

Corrado

et al. 2018

Sci Rep

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“…In contrast, only 39 ruminant PV types are recognized up to now (https://pave.niaid.nih.gov/), which leads us to support the importance of improving the efforts to characterize animal PVs. Recent studies, some using high‐throughput sequencing platforms have shown that animal PVs were underestimated and new types have been characterized (Bauermann et al., ; Da Silva et al., ; Daudt, da Silva, Streck, et al., ; López‐Bueno et al., ; Tore et al., ).…”

Section: Papillomaviruses Species In Ruminantsmentioning

confidence: 99%

“…The Bovidae family also consists of PVs that were characterized in three other host species: Capra hircus (ChPV1); Ovis aries (OaPV1, OaPV2, OaPV3 and the OaPV4 recently described (Tore et al., ); and Bos grunniens (BgPV1). The other Ruminantia PVs are sporadically diagnosed.…”

Section: Epidemiologymentioning

confidence: 99%

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Papillomaviruses in ruminants: An update

Daudt

Silva

Lunardi

et al. 2018

Transbound Emerg Dis

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Papillomaviruses (PVs) are complex viruses which infect the skin or mucosae of a broad range of amniotes worldwide. They cause benign or malignant lesions depending on environmental factors, virus oncogenicity and the location of infection. Bovine papillomaviruses (BPVs) are the second most studied PVs beyond human PVs. In the past few years, genetic characterization of animal PVs has increased due to the availability of new techniques, which simplified the sequencing of entire genomes. Therefore, this review aims to provide an update of the current epidemiology, classification and genome features of ruminant PVs (mainly BPVs) affecting animals worldwide. The review also aimed to clarify the key differences between the high-risk Delta papillomaviruses and the seemingly low-risk Xi, Epsilon, Dyoxi and Dyokappapillomavirus as well as the recently described PVs BPV18, 19, 21 and PpuPV1 that belongs to an unclassified genus.

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“…It has been documented that papillomas are rare in sheep (Maxie & Newman, ), and the viral type related to these lesions is not frequently diagnosed. Currently, the ovines PVs— Ovis aries papillomavirus (OaPVs)—are divided into two genera, being Deltapapillomavirus (OaPV1, OaPV2 and OaPV4) and Dyokappapapillomavirus (OaPV3) (Alberti et al., ; Tore et al., ). The Delta ‐PVs include very important viral types and have received considerable scientific attention for two main reasons.…”

Section: Introductionmentioning

confidence: 99%

First detection of bovine papillomavirus type 2 in cutaneous wart lesions from ovines

Mazzuchelli-de-Souza

Carvalho

Módolo

et al. 2018

Transbound Emerg Dis

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This study diagnosed cutaneous wart lesions excised from three rams from a sheep farm in São Paulo State, Brazil. Histopathologically, these cases were diagnosed as papilloma. The amplification by PCR, sequencing and bioinformatics analysis showed that all the lesions presented DNA sequences of bovine papillomavirus type 2. This is the first report confirming the detection of BPV2 in papilloma warts from ovines.

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Host cell tropism, genome characterization, and evolutionary features of OaPV4, a novel Deltapapillomavirus identified in sheep fibropapilloma

Cited by 23 publications

References 29 publications

Oral fibropapillomatosis and epidermal hyperplasia of the lip in newborn lambs associated with bovine Deltapapillomavirus

Oral fibropapillomatosis and epidermal hyperplasia of the lip in newborn lambs associated with bovine Deltapapillomavirus

Papillomaviruses in ruminants: An update

First detection of bovine papillomavirus type 2 in cutaneous wart lesions from ovines

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