HOS15 is a transcriptional corepressor of NPR1-mediated gene activation of plant immunity

Shen, Mingzhe; Lim, Chaehun; Park, Junghoon; Kim, Jeong Eun; Baek, Dongwon; Nam, Jaesung; Lee, Sang Yeol; Pardo, José M.; Kim, Woe‐Yeon; Mackey, David; Yun, Dae‐Jin

doi:10.1073/pnas.2016049117

Cited by 26 publications

(38 citation statements)

References 103 publications

(135 reference statements)

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“…Furthermore, HOS15 interacts with and degrades OST1, thereby regulating the desensitization of the ABA signaling pathway (Ali et al, 2019). In line with these reports, we have also shown that HOS15 interacts with the SCF-CUL4-E3 ligase complex to repress the plant immune system by negatively regulating NPR1, a pathogen-responsive positive regulator (Shen et al, 2020). Despite all these multiple functions, the role of HOS15 in senescence remains unknown.…”

Section: Introductionsupporting

confidence: 79%

“…High Expression of Osmotically Responsive Genes 15 (HOS15), a WD40 repeat protein, has multiple molecular functions, including the regulation of plant growth and development, cold stress signaling, flowering time determination, abscisic acid (ABA) signaling, response to drought stress, and pathogen response (Zhu et al, 2008;Park et al, 2018a;Ali et al, 2019;Mayer et al, 2019;Park et al, 2019;Shen et al, 2020). During cold stress, HOS15 interacts with and promotes proteasomal degradation of histone deacetylase 2C (HD2C).…”

Section: Introductionmentioning

confidence: 99%

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The Transcriptional Corepressor HOS15 Mediates Dark-Induced Leaf Senescence in Arabidopsis

et al. 2022

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Multiple endogenous and environmental signals regulate the intricate and highly complex processes driving leaf senescence in plants. A number of genes have been identified in a variety of plant species, including Arabidopsis, which influence leaf senescence. Previously, we have shown that HOS15 is a multifunctional protein that regulates several physiological processes, including plant growth and development under adverse environmental conditions. HOS15 has also been reported to form a chromatin remodeling complex with PWR and HDA9 and to regulate the chromatin structure of numerous genes. However, unlike PWR and HDA9, the involvement of HOS15 in leaf senescence is yet to be identified. Here, we report that HOS15, together with PWR and HDA9, promotes leaf senescence via transcriptional regulation of SAG12/29, senescence marker genes, and CAB1/RCBS1A, photosynthesis-related genes. The expression of ORE1, SAG12, and SAG29 was downregulated in hos15-2 plants, whereas the expression of photosynthesis-related genes, CAB1 and RCBS1A, was upregulated. HOS15 also promoted senescence through dark stress, as its mutation led to a much greener phenotype than that of the WT. Phenotypes of double and triple mutants of HOS15 with PWR and HDA9 produced phenotypes similar to those of a single hos15-2. In line with this observation, the expression levels of NPX1, APG9, and WRKY57 were significantly elevated in hos15-2 and hos15/pwr, hos15/hda9, and hos15/pwr/hda9 mutants compared to those in the WT. Surprisingly, the total H3 acetylation level decreased in age-dependent manner and under dark stress in WT; however, it remained the same in hos15-2 plants regardless of dark stress, suggesting that dark-induced deacetylation requires functional HOS15. More interestingly, the promoters of APG9, NPX1, and WRKY57 were hyperacetylated in hos15-2 plants compared to those in WT plants. Our data reveal that HOS15 acts as a positive regulator and works in the same repressor complex with PWR and HDA9 to promote leaf senescence through aging and dark stress by repressing NPX1, APG9, and WRKY57 acetylation.

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Section: Introductionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

The Transcriptional Corepressor HOS15 Mediates Dark-Induced Leaf Senescence in Arabidopsis

et al. 2022

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“…Hence, the discovery of common targets or susceptibility genes for pathogens in crop plants could now be a very important focus. In fact, pathogens sharing the same plant targets have been frequently reported (Ali and Yun, 2020; Park et al ., 2019; Ray et al ., 2019; Shen et al ., 2020). Our study provides an approach to develop highly resistant plants against multiple pathogens by editing only a single gene.…”

Section: Discussionmentioning

confidence: 99%

Editing homologous copies of an essential gene affords crop resistance against two cosmopolitan necrotrophic pathogens

Zhang

Chéng

Lin

et al. 2021

Plant Biotechnology Journal

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Sclerotinia sclerotiorum and Botrytis cinerea are typical necrotrophic pathogens that can attack more than 700 and 3000 plant species, respectively, and cause huge economic losses across numerous crops. In particular, the absence of resistant cultivars makes the stem rot because of S. sclerotiorum the major threat of rapeseed (Brassica napus) worldwide along with Botrytis. Previously, we identified an effector‐like protein (SsSSVP1) from S. sclerotiorum and a homologue of SsSSVP1 on B. cinerea genome and found that SsSSVP1 could interact with BnQCR8 of rapeseed, a subunit of the cytochrome b‐c1 complex. In this study, we found that BnQCR8 has eight homologous copies in rapeseed cultivar Westar and reduced the copy number of BnQCR8 using CRISPR/Cas9 to improve rapeseed resistance against S. sclerotiorum. Mutants with one or more copies of BnQCR8 edited showed strong resistance against S. sclerotiorum and B. cinerea. BnQCR8‐edited mutants did not show significant difference from Westar in terms of respiration and agronomic traits tested, including the plant shape, flowering time, silique size, seed number, thousand seed weight and seed oil content. These traits make it possible to use these mutants directly for commercial production. Our study highlights a common gene for breeding of rapeseed to unravel the key hindrance of rapeseed production caused by S. sclerotiorum and B. cinerea. In contrast to previously established methodologies, our findings provide a novel strategy to develop crops with high resistance against multiple pathogens by editing only a single gene that encodes the common target of pathogen effectors.

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“…Functional deficiencies in CSN result in strong pleiotropic effects including auxin-insensitivity (Dohmann et al ., 2008), constitutive activation of PSR (Walia et al, 2020), and impaired JA-defenses accompanied by elevated PR1 transcripts (Hind et al ., 2011), thus revealing strong parallel with ipk1-1 or itpk1-2 defects. Rice SPX4, SNC1 or NPR1 stabilities are monitored by CRL functions placing molecular implications of IPK1/ITPK1 on defense outcomes via CSN-CRL dynamics (Gou et al ., 2012; Ruan et al ., 2019; Ried et al ., 2019; Shen et al ., 2020). These indications lead us to advocate that between the investigated InsP-kinases, IPK1 and ITPK1 have acquired unique localized roles that impinge on central cellular machineries impacting multiple signaling networks.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis inositol polyphosphate kinases IPK1 and ITPK1 modulate crosstalks between SA-dependent immunity and phosphate-starvation responses

Gulabani

Goswami²,

Walia³

et al. 2021

Preprint

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The propensity for polyphosphorylation makes myo-inositol derivatives, the inositol polyphosphates (InsPs), especially phytic acid or inositol hexakisphosphate (InsP6) the major form of phosphate storage in plants. Acts of pyrophosphorylation on InsP6 generates InsP7 or InsP8 containing high-energy phosphoanhydride bonds that are harnessed during energy requirements of a cell. Also implicated as co-factors for several phytohormone signaling networks, InsP7/InsP8 modulate key developmental processes. With recent identification as the common moeity for transducing both jasmonic acid (JA) and phosphate-starvation responses (PSR), InsP8 is the classic example of a metabolite that may moonlight crosstalks to different cellular pathways during diverse stress adaptations. We show here that Arabidopsis thaliana INOSITOL PENTAKISPHOSPHATE 2-KINASE (IPK1), INOSITOL 1,3,4-TRISPHOSPHATE 5/6-KINASE 1 (ITPK1), and DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE 2 (VIH2), but not other InsP-kinases, suppress basal salicylic acid (SA)-dependent immunity. In ipk1, itpk1 or vih2 mutants, elevated endogenous SA levels and constitutive activation of defense signaling lead to enhanced resistance against the virulent Pseudomonas syringae pv tomato DC3000 (PstDC3000) strain. Our data reveal that activated SA-signaling sectors in these mutants modulate expression amplitudes of phosphate-starvation inducible (PSI)-genes, reported earlier. In turn, via mutualism the heightened basal defenses in these mutants require upregulated PSI-gene expressions likely highlighting the increased demand of phosphates required to support immunity. We demonstrate that SA is induced in phosphate-deprived plants, however its defense-promoting functions are likely diverted to PSR-supportive roles. Overall, our investigations reveal selective InsPs as crosstalk mediators among diverse signaling networks programming stress-appropriate adaptations.

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HOS15 is a transcriptional corepressor of NPR1-mediated gene activation of plant immunity

Cited by 26 publications

References 103 publications

The Transcriptional Corepressor HOS15 Mediates Dark-Induced Leaf Senescence in Arabidopsis

The Transcriptional Corepressor HOS15 Mediates Dark-Induced Leaf Senescence in Arabidopsis

Editing homologous copies of an essential gene affords crop resistance against two cosmopolitan necrotrophic pathogens

Arabidopsis inositol polyphosphate kinases IPK1 and ITPK1 modulate crosstalks between SA-dependent immunity and phosphate-starvation responses

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