Horseradish peroxidase-functionalized Pt hollow nanospheres and multiple redox probes as trace labels for a sensitive simultaneous multianalyte electrochemical immunoassay

Abstract

A novel strategy for a simultaneous multianalyte electrochemical immunoassay is proposed using thionine-labeled anti-AFP and ferrocene-labeled anti-CEA as tracers; each biorecognition event yielded a distinct voltammetric peak, whose position and size reflected the identify and level of the corresponding antigen.

Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition

“…The correlation coefficients were 0.9992 and 0.9983 for AFP and CEA, respectively. The limits of detection for AFP and CEA were 0.024 and 0.032 pg mL À1 , respectively, which were much lower than those reported in the previous studies [34,41,42].…”

An amplified electrochemical immunosensor based on in situ-produced 1-naphthol as electroactive substance and graphene oxide and Pt nanoparticles functionalized CeO2 nanocomposites as signal enhancer

“…Among them, sandwich-type immunosensor has been received much attention in recent years because of its improved sensitivity originated from different signal amplified strategies (Dutta et al, 2014;Liu et al, 2013;Lin et al, 2013;Cao et al, 2013). However, the detectable signal of conventional sandwich-type immunosensor must be obtained from the labeled electroactive substance such as 3 metal nanoparticles (ZnS, PbS, CdS) (Dai et al, 2011;) and redox-active small molecules (thionine, toluidine blue and prussian blue) (Han et al, 2013;Song et al, 2010;Wang et al, 2014). Compared with labeling macromolecular, the small molecules labeled in these immunosensors exhibit more heterogeneity and easily peel off from electrode surface to the solution, resulting in unstable output signal, poor reproducibility in electrochemical performance and increase of the analytical time (Liu et al, 2004).…”

Metallic nanocrystallites-incorporated ordered mesoporous carbon as labels for a sensitive simultaneous multianalyte electrochemical immunoassay

“…Generally, for diagnosis, the detection of one type of tumor markers could cause false-positive or false-negative results. Therefore, simultaneous detection of the tumor markers have recently aroused great interest in multiplexed assays and diagnostics, which is one of the leading trends for further development of faster and more accurate immunosensing devices Freeman et al, 2009;Song et al, 2010;Li et al, 2012a;Tang et al, 2011).…”