Horseradish peroxidase functionalized gold nanorods as a label for sensitive electrochemical detection of alpha-fetoprotein antigen

Guo, Jinjin; Han, Xiaowei; Wang, Junchun; Zhao, Junqing; Guo, Zilin; Zhang, Yu-Zhong

doi:10.1016/j.ab.2015.09.006

Cited by 42 publications

(11 citation statements)

References 22 publications

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“…16,17 Thus, developing rapid and simple methods for the detection of AFP is of great clinical value. Traditional methods for the detection of AFP include enzymelinked immunosorbent assay (ELISA), 18 electrochemistry, [19][20][21] electrochemiluminescence, 22 quartz crystal microbalance, 23 and surface plasmon resonance (SPR) immunoassays. 24 Among these methods, electrochemistry has gained signicant interest due to its simple instrumentation, low cost and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

A label-free electrochemical immunosensor for the detection of cancer biomarker α-fetoprotein (AFP) based on hydroxyapatite induced redox current

et al. 2016

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Section: Introductionmentioning

confidence: 99%

A label-free electrochemical immunosensor for the detection of cancer biomarker α-fetoprotein (AFP) based on hydroxyapatite induced redox current

et al. 2016

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“…The immunoassay protocol is illustrated in Scheme 1. First, Au NPs were deposited on the surface of cleaned GCE (Notes: Au NPs/GCE) (the bare electrode was treated according to our previous reported 22 ). The reason for depositing Au NPs is that Au NPs possess good conductivity and high surface areas which could improve the conductivity of the electrode interface and load amount of primary antibody (Ab 1 ).…”

Section: Methodsmentioning

confidence: 99%

Ultrasensitive Multiplexed Immunoassay for Tumor Biomarkers Based on DNA Hybridization Chain Reaction Amplifying Signal

Wang

Zhao

Guo

et al. 2016

ACS Appl. Mater. Interfaces

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In this work, a novel electrochemical immunoassay protocol has been reported for simultaneous determination of multiple tumor biomarkers based on DNA hybridization chain reaction (HCR) for signal amplification. Alpha-fetoprotein (AFP) and prostate specific antigen (PSA) were selected as model biomarkers. The immunoassay protocol contained primary antibodies immobilized on gold nanoparticles (Au NPs), secondary antibodies conjugated with DNA concatemer from HCR of primer, auxiliary probe, and signal probe labeled with signal molecules (methyleneblue (MB) and ferrocene (Fc)). In the presence of target biomarkers, the sandwich immunocomplex was formed between the primary antibodies and secondary antibodies bioconjugates carrying numerous signal molecules. As a result, two well-resolved reduction peaks, one was at -0.35 V (corresponding to MB) and other was at 0.33 V (corresponding to Fc; both vs SCE), were obtained in differential pulse voltammetry, and peak currents changed were related to the level of biomarkers. Under optimal conditions, the electrochemical immunoassay exhibited a wide linear response range (0.5 pg mL(-1) to 50 ng mL(-1)) and low detection limits (PSA, 0.17 pg mL(-1); AFP, 0.25 pg mL(-1)) (at S/N = 3). In addition, the immunoassay was evaluated by analyzing simulate human serum sample, and the recoveries obtained were within 99.4-107.6% for PSA and 97.9-108.2% for AFP, indicating the immnuoassay could be applied to the simultaneous detection of AFP and PSA in human serum samples.

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“…, Guo et al. and Zhong et al. used similar methodologies based on HRP as label and 3,3′,5,5′ tetramethylbenzidine (TMB) as co‐substrate.…”

Section: Assay Formats (Immunochemical Interactions)mentioning

confidence: 99%

“…Furthermore, the conjugation between gold‐ and carbon‐based nanomaterials and/or ionic liquids offer multiple options in the sensor's construction. Improved performances were achieved using distinct combinations, such as AuNC/nickel hexacyanoferrate NPs/AuNP‐GS , MWCNTs/AuNPs , AuNPs/Fc‐rGO , AuNPs‐porous GO , MWCNTs/gold colloids , Nafion/MWCNTs/AuNPs , AuNRs/CNTs , gold nanowires (AuNW)/GS , chitosan (CS)‐SWCNTs/AuNPs , rGO/nafion/AuNPs , AuNPs/PDA/TH/GO .…”

Section: Electrode Surface Modificationmentioning

confidence: 99%

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

2018

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In cancer, screening and early detection are critical for the success of the patient's treatment and to increase the survival rate. The development of analytical tools for non‐invasive detection, through the analysis of cancer biomarkers, is imperative for disease diagnosis, treatment and follow‐up. Tumour biomarkers refer to substances or processes that, in clinical settings, are indicative of the presence of cancer in the body. These biomarkers can be detected using biosensors, that, because of their fast, accurate and point of care applicability, are prominent alternatives to the traditional methods. Moreover, the constant innovations in the biosensing field improve the determination of normal and/or elevated levels of tumour biomarkers in patients’ biological fluids (such as serum, plasma, whole blood, urine, etc.). Although several biomarkers (DNA, RNA, proteins, cells) are known, the detection of proteins and circulating tumour cells (CTCs) are the most commonly reported due to their approval as tumour biomarkers by the specialized entities and commonly accepted for diagnosis by medical and clinical teams. Therefore, electrochemical immunosensors and cytosensors are vastly described in this review, because of their fast, simple and accurate detection, the low sample volumes required, and the excellent limits of detection obtained. The biosensing strategies reported for the six most commonly diagnosed cancers (lung, breast, colorectal, prostate, liver and stomach) are summarized and the distinct phases of the sensors’ constructions (surface modification, antibody immobilization, immunochemical interactions, detection approach) and applications are discussed.

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Horseradish peroxidase functionalized gold nanorods as a label for sensitive electrochemical detection of alpha-fetoprotein antigen

Cited by 42 publications

References 22 publications

A label-free electrochemical immunosensor for the detection of cancer biomarker α-fetoprotein (AFP) based on hydroxyapatite induced redox current

A label-free electrochemical immunosensor for the detection of cancer biomarker α-fetoprotein (AFP) based on hydroxyapatite induced redox current

Ultrasensitive Multiplexed Immunoassay for Tumor Biomarkers Based on DNA Hybridization Chain Reaction Amplifying Signal

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

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