Hormone metabolism and action: Testosterone and metabolites in prostate organ culture

Röbel, P; Lasnitzki, Ilse; Bauliec, Etienne-Emile

doi:10.1016/s0300-9084(71)80086-x

Cited by 156 publications

(38 citation statements)

References 24 publications

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“…Human tissues (BPH and carcinoma) were used as soon as possible after surgery. The methodology for organ culture was basically similar to that previously described by others [18,20]. For the prepara tion of rat tissue, the ventral and dorsolateral lobes of the prostate were teased apart into 2 mm3 fragments and placed on siliconized lens paper.…”

Section: Methodsmentioning

confidence: 99%

5α-Reductase as a Target Enzyme for Anti-Prostatic Drugs in Organ Culture

Kadohama¹,

Kirdani²,

Murphy³

et al. 1977

Oncology

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Short-term organ culture of rat and human prostatic tissues has been utilized as a means of testing drugs potentially useful in cancer of the prostate. Optimal conditions, particularly the concentration of T, have been established for organ culture of such tissues and the effects of various drugs on 5α-reductase activity (5α-RA) have been utilized as a means of ascertaining antiprostatic actions of the various compounds and drugs tested. Thus, it was shown that estracyt, estradiol-17β, progesterone, and novel steroids and steroid-conjugates have definite effects on 5α-RA in this system.

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Section: Methodsmentioning

confidence: 99%

5α-Reductase as a Target Enzyme for Anti-Prostatic Drugs in Organ Culture

Kadohama¹,

Kirdani²,

Murphy³

et al. 1977

Oncology

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“…2.24 mg/ml Na2COj, 0.82 pg/ml testosterone propionate [9], 0.167 pg/ml hydrocortisone succinate [7], and 6.6 X 10_>U/ml insulin. Antibiotics included penicillin 82 U/ml, streptomycin 82 pg/ml.…”

Section: Culture Techniquementioning

confidence: 99%

Tissue Culture of Normal Human Post-Pubertal Prostate

Jacobs

Lawson

1981

Urol Int

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Total prostatectomies were performed in 10 young male cadaver renal donors and explant cultures were prepared in Falcon flasks. Growth of a monolayer of predominantly epithelial cells occurred rapidly (latent time 3–5 days) and the flasks became confluent within 15–17 days. The monolayers grew well even when a needle was used to circumscribe and remove the explants. Passaging of monolayer cells by seeding at a density of 5 × 10⁵ resulted in the vigorous growth of epithelial cells. However, the cultures were eventually overgrown by fibroblasts. Similar explant cultures of benign prostatic hyperplasia or prostatic carcinoma demonstrated a longer latent time, rarely attained confluence, died after explant removal, and could not be passaged.

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“…Their formation may be a factor controlling the amount of T-active metabolite inside the androgen target cell. Although DHT is generally considered to be the T-active metabolite at the skin level, 3p-diol has been reported to regulate the secretion processes in the rat sebaceous gland [19,20] and prostate [21], These considerations formed the rationale behind the present study, to determine 3P-diol metabolism in hairy and bald scalp, the latter being generally considered to be 'enriched' in sebaceous glands.…”

Section: Introductionmentioning

confidence: 98%

Metabolism of 5α-Androstane-3β, 17β-diol in Bald and HairyAreas of the Scalp

Caballero¹

1994

Horm Res

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In androgen target tissues, testosterone undergoes extensive biotransformation to 5α-dihydrotestosterone which in turn is metabolized into 5α-androstane-3α, 17β-diol and 5α-androstane-3β, 17β-diol. From studies mainly in the prostate, it has been shown that 5α-androstane-3β, 17β-diol can be further converted to 5α-androstane-3β,6α(β)/7α(β), 17β-triols. The presentstudies show that among the metabolites of 5α-androstane-3β, 17β-diol in scalp homogenates, three were isolated by thin-layer chromatography and identified against standard steroids: 5α-dihydrotestosterone, epiandrosterone, and 5α-androstanedione. Another peak was observed near the origin with a greater polarity than 5α-androstane-3β, 17β-diol. The chromatograms obtained by incubating 5α-androstane-3β,17β-diol with scalp and prostate homogenates are similar, and it is known that the polar metabolites in the prostate are 5α-androstane-3β,6α(β)/7α(β),17β-triols, suggesting that these steroids are formed in both tissues. In addition, the similarity of chromatograms and mass spectra, obtained when 3β-diol polar metabolite(s) and authentic 5α-androstane-3β,7β,17β-triol were subjected to GC/MS, confirms this hypothesis. The dependence of the formation of polar metabolites on the substrate (5α-androstane-3β,17β-diol) concentration (50 nM-1.3 μM) was studied and resulted in saturation and linear Lineweaver plots from both bald and hairy areas of the scalp. The mean Michaelis constant (K_m) was 1 μM for both areas, and the maximal metabolic rates (V_max) were 10.9 ± 6.2 pmol/mg protein/h (n = 9) in bald scalp and 8.2 ± 0.1 pmol/mg protein/h (n = 3) in hairy scalp. The other metabolic rates of formation were: 25.8 ± 19.5-38.4 ± 17.9 pmol/mg protein/h for epiandrosterone, 45.8 ± 40.9-8.4 ± 2.2 pmol/mg protein/h for 5α-dihydrotestosterone, and 8.3 ± 2.6-9.2 ± 1.0 pmol/mg protein/h for androstanedione in bald and hairy scalp, respectively. As 5α-androstane-3β,6α(β)/7α(β),17β-triols do not possess androgenic properties, they are generally considered to be final metabolites, so that their production rate could be an important factor controlling the amount of 5α-androstane-3β, 17β-diol inside the target cell.

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Hormone metabolism and action: Testosterone and metabolites in prostate organ culture

Cited by 156 publications

References 24 publications

5α-Reductase as a Target Enzyme for Anti-Prostatic Drugs in Organ Culture

5α-Reductase as a Target Enzyme for Anti-Prostatic Drugs in Organ Culture

Tissue Culture of Normal Human Post-Pubertal Prostate

Metabolism of 5α-Androstane-3β, 17β-diol in Bald and HairyAreas of the Scalp

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Hormone metabolism and action: Testosterone and metabolites in prostate organ culture

Cited by 156 publications

References 24 publications

5α-Reductase as a Target Enzyme for Anti-Prostatic Drugs in Organ Culture

5α-Reductase as a Target Enzyme for Anti-Prostatic Drugs in Organ Culture

Tissue Culture of Normal Human Post-Pubertal Prostate

Metabolism of 5&alpha;-Androstane-3&beta;, 17&beta;-diol in Bald and HairyAreas of the Scalp

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Metabolism of 5α-Androstane-3β, 17β-diol in Bald and HairyAreas of the Scalp