Hormonal regulation of ciliary function in the oviduct: The effect of beta-adrenergic agonists

Villalón, Manuel; Verdugo, Pedro

doi:10.1002/cm.970020713

Cited by 12 publications

(7 citation statements)

References 29 publications

(11 reference statements)

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“…The Doppler-shifted light scattered by the moving cilia was collected by the objective lens aperture of the microscope at an angle of 300 and detected with a photomultiplier tube. A detailed description of the instrumentation has been published elsewhere Villal6n and Verdugo, 1982;Verdugo and Golborne, 1988). The spectral structure of the scattering fluctuations was processed on line by a fast Fourier transform (FFT) digital spectrum analyzer designed in our laboratory.…”

Section: Methodsmentioning

confidence: 99%

Stimulus-response coupling in mammalian ciliated cells. Demonstration of two mechanisms of control for cytosolic [Ca2+]

Villalón

Hinds

Verdugo

1989

Biophysical Journal

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Changes of cytosolic [Ca2+] have been proposed to couple stimulation of ciliary movement, however, quantitative measurements of fluctuations of intracellular free [Ca2+] associated with stimulation of ciliated cells have not been investigated. In primary cultures of rabbit oviductal ciliated cells, the stimulation of ciliary activity produced by micromolar concentrations of adenosine triphosphate (ATP) and prostaglandin F2 alpha (PGF2 alpha) was associated with a transient increase of intracellular [Ca2+]. Whereas the increase of cytosolic [Ca2+] and beat frequency produced by ATP were inhibited by the Ca-channel blocker LaCl3, the rise of cytosolic [Ca2+] and frequency of ciliary beat produced by PGF2 alpha was not affected by LaCl3. These results are the first direct demonstration that fluctuations of cytosolic [Ca2+] are associated with increased ciliary beat frequency in mammalian epithelial cells. The present findings suggest two different calcium-dependent mechanisms for stimulus-coupling in ciliary epithelium: ATP acting via purinergic receptor coupled to transmembrane influx of Ca2+, and PGF2 alpha acting via receptor-mediated release of intracellular sequestered Ca.

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Section: Methodsmentioning

confidence: 99%

Stimulus-response coupling in mammalian ciliated cells. Demonstration of two mechanisms of control for cytosolic [Ca2+]

Villalón

Hinds

Verdugo

1989

Biophysical Journal

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“…The male sperm has a long tail, and it is a flagellum [21]. Cilia in the female oviduct assist in the transport of the ovum [22]. Cilia in the animal respiratory system stop infectious pathogenic organisms and help move them out of the lung airway [23].…”

Section: Cilia and Mucusmentioning

confidence: 99%

Mathematical Modeling of Mucociliary Clearance: A Mini-Review

Jiang

2019

Cells

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Mucociliary clearance is an important innate host defense of the mammalian respiratory system, as it traps foreign substances, including pollutants, pathogens, and allergens, and transports them out of the airway. The underlying mechanism of the actuation and coordination of cilia, the interplay between the cilia and mucus, and the formation of the metachronal wave have been explored extensively both experimentally and mathematically. In this mini-review, we provide a survey of the mathematical models of mucociliary clearance, from the motion of one single cilium to the emergence of the metachronal wave in a group of them, from the fundamental theoretical study to the state-of-the-art three-dimensional simulations. The mechanism of cilium actuation is discussed, together with the mathematical simplification and the implications or caveats of the results.

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“…Indeed, pathologies that affect motile cilia activity (e.g., primary ciliary dyskinesia) are characterized by impaired fertility along with impaired airway mucociliary clearance [ 69 ]. Ciliary beat frequency (CBF) is regulated by several stimuli (e.g., calcium levels, adenosine triphosphate, angiotensin II, and β-adrenergic stimuli) [ 70 , 71 ]; high levels of estrogen in the pre-ovulatory phase increase CBF and ciliogenesis, whereas high levels of progesterone induce deciliation and decrease CBF [ 71 , 72 , 73 , 74 , 75 , 76 , 77 ]. Remarkably, follicular fluid generates a genotoxic stress in fallopian epithelial cells due to an oxidative burst after ovulation [ 50 , 57 , 78 ]; at the same time, CBF increases immediately after ovulation, as does the speed of laminal fluid flow above the FTE [ 76 ].…”

Section: Ciliated Cells In the Fallopian Tube: Function And Tumor mentioning

confidence: 99%

Exploring the Role of Fallopian Ciliated Cells in the Pathogenesis of High-Grade Serous Ovarian Cancer

Coan

Vinciguerra

Cesaratto

et al. 2018

IJMS

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High-grade serous epithelial ovarian cancer (HGSOC) is the fifth leading cause of cancer death in women and the first among gynecological malignancies. Despite an initial response to standard chemotherapy, most HGSOC patients relapse. To improve treatment options, we must continue investigating tumor biology. Tumor characteristics (e.g., risk factors and epidemiology) are valuable clues to accomplish this task. The two most frequent risk factors for HGSOC are the lifetime number of ovulations, which is associated with increased oxidative stress in the pelvic area caused by ovulation fluid, and a positive family history due to genetic factors. In the attempt to identify novel genetic factors (i.e., genes) associated with HGSOC, we observed that several genes in linkage with HGSOC are expressed in the ciliated cells of the fallopian tube. This finding made us hypothesize that ciliated cells, despite not being the cell of origin for HGSOC, may take part in HGSOC tumor initiation. Specifically, malfunction of the ciliary beat impairs the laminar fluid flow above the fallopian tube epithelia, thus likely reducing the clearance of oxidative stress caused by follicular fluid. Herein, we review the up-to-date findings dealing with HGSOC predisposition with the hypothesis that fallopian ciliated cells take part in HGSOC onset. Finally, we review the up-to-date literature concerning genes that are located in genomic loci associated with epithelial ovarian cancer (EOC) predisposition that are expressed by the fallopian ciliated cells.

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Hormonal regulation of ciliary function in the oviduct: The effect of beta-adrenergic agonists

Cited by 12 publications

References 29 publications

Stimulus-response coupling in mammalian ciliated cells. Demonstration of two mechanisms of control for cytosolic [Ca2+]

Stimulus-response coupling in mammalian ciliated cells. Demonstration of two mechanisms of control for cytosolic [Ca2+]

Mathematical Modeling of Mucociliary Clearance: A Mini-Review

Exploring the Role of Fallopian Ciliated Cells in the Pathogenesis of High-Grade Serous Ovarian Cancer

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