Hormonal regulation of cereal endosperm development with a focus on rice (Oryza sativa)

Basunia, Mafroz Ahmed; Nonhebel, Heather M.

doi:10.1071/fp18323

Cited by 35 publications

(25 citation statements)

References 111 publications

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“…as evidenced in Joshi et al , ). Basunia and Nonhebel () also emphasized this issue of inadequate analyses with regard to auxin and cytokinin analyses.…”

Section: Cytokininsmentioning

confidence: 99%

Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

Chen

Zhao

Song

et al. 2019

Plant Biotechnology Journal

110

131

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J.S.)) and (Tel +64 275553582; email paula.jameson@canterbury.ac.nz (P.E.J.)) SummaryThe plant hormone group, the cytokinins, is implicated in both qualitative and quantitative components of yield. Cytokinins have opposing actions in shoot and root growth-actions shown to involve cytokinin dehydrogenase (CKX), the enzyme that inactivates cytokinin. We revise and provide unambiguous names for the CKX gene family members in wheat, based on the most recently released wheat genome database, IWGSC RefSeq v1.0 & v2.0. We review expression data of CKX gene family members in wheat, revealing tissue-specific gene family member expression as well as sub-genome-specific expression. Manipulation of CKX in cereals shows clear impacts on yield, root growth and orientation, and Zn nutrition, but this also emphasizes the necessity to unlink promotive effects on grain yield from negative effects of cytokinin on root growth and uptake of mineral nutrients, particularly Zn and Fe. Wheat is the most widely grown cereal crop globally, yet is under-research compared with rice and maize. We highlight gaps in our knowledge of the involvement of CKX for wheat. We also highlight the necessity for accurate analysis of endogenous cytokinins, acknowledging why this is challenging, and provide examples where inadequate analyses of endogenous cytokinins have led to unjustified conclusions. We acknowledge that the allohexaploid nature of bread wheat poses challenges in terms of uncovering useful mutations. However, we predict TILLING followed by whole-exome sequencing will uncover informative mutations and we indicate the potential for stacking mutations within the three genomes to modify yield components. We model a wheat ideotype based on CKX manipulation. 614

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“…as evidenced in Joshi et al , ). Basunia and Nonhebel () also emphasized this issue of inadequate analyses with regard to auxin and cytokinin analyses.…”

Section: Cytokininsmentioning

confidence: 99%

Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

Chen

Zhao

Song

et al. 2019

Plant Biotechnology Journal

110

131

View full text Add to dashboard Cite

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“…8A; Supplemental Table S5). Previous work has shown that ABCB4/11/14/22, DAO, GH3s, and IAAs are up-regulated in developing rice seeds at 24 HAF (Jain and Khurana, 2009;Uchiumi and Okamoto, 2010;French et al, 2014;Basunia and Nonhebel, 2019). TRYPTOPHAN AMINOTRANSFER-ASE-RELATED PROTEIN2 (TAR2), the auxin biosynthesis gene that is preferentially expressed prior to cellularization (Abu-Zaitoon et al, 2012), exhibited lower transcript levels in MADS78 and MADS79 mutants, while YUCCA7 was unaltered in the MADS78 mutant and showed higher transcript abundance in the MADS79 mutant (Fig.…”

Section: Expression Of Mads78 and Mads79 Is Negatively Associated Witmentioning

confidence: 95%

“…Early cellularization in the absence of MADS78 or MADS79 triggers activation of the CArG box-containing auxin biosynthesis genes YUCCA12 and TAR1 (Fig. 8A) that may have a role in subsequent endoreduplication, differentiation, and starch deposition during later stages of seed development (Abu-Zaitoon et al, 2012;Basunia and Nonhebel, 2019). Detection of CArG box-binding motifs in promoters of auxin biosynthesis enzyme-, transporter-, and signaling-related genes further supports the regulatory role of type I MADS box TFs in maintaining auxin levels and/or distribution (Supplemental Table S5).…”

Section: Interplay Between Auxin Type I Mads Box Tfs and Fis-prc2 Ementioning

confidence: 99%

MADS78 and MADS79 Are Essential Regulators of Early Seed Development in Rice

et al. 2019

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MADS box transcription factors (TFs) are subdivided into type I and II based on phylogenetic analysis. The type II TFs regulate floral organ identity and flowering time, but type I TFs are relatively less characterized. Here, we report the functional characterization of two type I MADS box TFs in rice (Oryza sativa), MADS78 and MADS79. Transcript abundance of both these genes in developing seed peaked at 48 h after fertilization and was suppressed by 96 h after fertilization, corresponding to syncytial and cellularized stages of endosperm development, respectively. Seeds overexpressing MADS78 and MADS79 exhibited delayed endosperm cellularization, while CRISPR-Cas9-mediated single knockout mutants showed precocious endosperm cellularization. MADS78 and MADS79 were indispensable for seed development, as a double knockout mutant failed to make viable seeds. Both MADS78 and 79 interacted with MADS89, another type I MADS box, which enhances nuclear localization. The expression analysis of Fie1, a rice FERTILIZATION-INDEPENDENT SEED-POLYCOMB REPRESSOR COMPLEX2 component, in MADS78 and 79 mutants and vice versa established an antithetical relation, suggesting that Fie1 could be involved in negative regulation of MADS78 and MADS79. Misregulation of MADS78 and MADS79 perturbed auxin homeostasis and carbon metabolism, as evident by misregulation of genes involved in auxin transport and signaling as well as starch biosynthesis genes causing structural abnormalities in starch granules at maturity. Collectively, we show that MADS78 and MADS79 are essential regulators of early seed developmental transition and impact both seed size and quality in rice.

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“…Our RNA-seq analysis results, revealed that several auxin biosynthesis genes, including YUCs and TARs, were downregulated in grains of RGB1Ri lines either at 5 DAF or 10 DAF (Figure 3A). There is evidence that IAA plays several distinct roles at different stages of endosperm development (see review by Figueiredo and Köhler, 2018;Basunia and Nonhebel, 2019). These differing roles require strong localized control of IAA biosynthesis by specific enzymes.…”

Section: Rgb1 Stimulates Accumulation Of Iaa By Regulating the Expression Of Auxin Biosynthesis-related Genesmentioning

confidence: 99%

RGB1 Regulates Grain Development and Starch Accumulation Through Its Effect on OsYUC11-Mediated Auxin Biosynthesis in Rice Endosperm Cells

Zhang

Liang

2021

Front. Plant Sci.

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RGB1, a subunit of heterotrimeric G protein, plays important roles in regulating grain size and weight of rice. However, the molecular mechanisms underlying controlling grain filling process by G protein are still largely unclear. In the present study, we show that RGB1 controls not only the grain size but also the grain filling process. Knock-down of RGB1 significantly delayed grain development and reduced starch accumulation and grain weight, which was closely related to the delayed and the lower expression of genes encoding sucrose metabolism and starch biosynthesis related enzymes during grain filling stage. Suppression of RGB1 expression also resulted in the lower auxin content in grains, which was correlated with the lower expression of OsNF-YB1 and OsYUC11 during grain filling stage. Further biochemical evidence showed that OsYUC11 expression was under control of OsNF-YB1 by its interaction with promoter of OsYUC11. Taken together, we propose that RGB1 controls rice grain development and grain filling process by changing auxin homeostasis in endosperm cells. OsNF-YB1, which acts as a key downstream effector of RGB1, interacts directly with the promoter of OsYUC11 and stimulates the OsYUC11 expression, thereby regulating auxin biosynthesis and starch accumulation and grain size.

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Hormonal regulation of cereal endosperm development with a focus on rice (Oryza sativa)

Cited by 35 publications

References 111 publications

Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

Cytokinin dehydrogenase: a genetic target for yield improvement in wheat

MADS78 and MADS79 Are Essential Regulators of Early Seed Development in Rice

RGB1 Regulates Grain Development and Starch Accumulation Through Its Effect on OsYUC11-Mediated Auxin Biosynthesis in Rice Endosperm Cells

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