Hormonal control of triterpenols synthesis in Euphorbia characias calli

Fernandes-Ferreira, Manuel; Novais, J. M.; Pais, M

doi:10.1016/0960-8524(92)90053-z

Cited by 6 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This may probably be due to toxic effects of the growth regulators as growth of these flax seedlings was significantly reduced when compared with that of seedlings grown on MS0 medium. The toxic effect on sterol production due to overdose levels or antagonism of auxins and cytokinins has been reported for other species (17,29,30).…”

Section: 29)mentioning

confidence: 99%

“…Phytosterols are important components of cellular membranes (14,15). The production of plant sterols in in vitro cultures has been extensively reported (16,17,18,19,20,21,22,23), however, studies on the 3 synthesis and accumulation of phytosterols associated with the induction and development of somatic embryos and shoot organogenesis have not been carried out.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax

Cunha

Fernandes-Ferreira

1997

Plant Science

View full text Add to dashboard Cite

A single medium was used for induction of somatic embryogenesis and shoot organogenesis from flax hypocotyls. With this system, a comparative study was performed to determine the free sterols content and composition in non-organogenic, shoot organogenic and embryogenic calli as well as in regenerated shoots and somatic embryos grown under the same hormonal supplementation. The induction of somatic embryogenesis and shoot organogenesis of flax was associated with an increase of total sterols in the competent calli and an increased ratio of stigmasterol to β-sitosterol in derived embryos and shoots. On the contrary, in non-organogenic calli, the ratio stigmasterol to β-sitosterol decreased during the exponential growth phase due to a drastic rise in the β-sitosterol content. This effect as well as the calli growth were higher with 0.5 mg/l 2,4-D than with 0.6 mg/l IBA.

show abstract

Section: 29)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax

Cunha

Fernandes-Ferreira

1997

Plant Science

View full text Add to dashboard Cite

show abstract

“…It has been established that some auxins generally stimulate the biosynthesis of both triterpenes (free and bound) and steroid compounds (Fernandes-Ferreira et al, 1992;Brain and Lockwood, 1976;Geuns, 1975). The experiments carried out during this project showed the effect of 5 auxins and their respective concentrations in the medium on the type of the synthesized isoprene derivative and its concentration in the V. corymbosum var.…”

Section: Resultsmentioning

confidence: 99%

The Influence of Auxins on the Biosynthesis of Isoprene Derivatives in Callus Cultures of Vaccinium corymbosum var. bluecrop

Migas

Łuczkiewicz

Cisowski

2006

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

Callus cultures of Vaccinium corymbosum var. bluecrop were optimized for their isoprene derivatives production by supplementing Schenk-Hildebrandt (SH) medium with constant concentration of kinetin (2.32 μm) and two different amounts of selected auxins. Every auxin, except for IBA, used in 10-time higher concentration (2,4D, NAA, IAA, NOA) stimulated biosynthesis of -sitosterol and inhibited triterpene synthesis. Quantitative analysis of isoprene derivatives in callus biomass collected on the 25 th day of the experiment proved that the analyzed callus of Vaccinium corymbosum var. bluecrop synthesized the highest amount of isoprene derivatives after subculturing on SH medium modified with 22.6 μm of 2,4D and 2.32 μm of kinetin.

show abstract

Euphorbia characias L.: In Vitro Culture and the Production of Sterols, Triterpenols, and Hydrocarbon-Like Compounds

Fernandes-Ferreira

Novais

Pais

1994

Medicinal and Aromatic Plants VII

View full text Add to dashboard Cite

Hormonal control of triterpenols synthesis in Euphorbia characias calli

Cited by 6 publications

References 23 publications

Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax

Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax

The Influence of Auxins on the Biosynthesis of Isoprene Derivatives in Callus Cultures of Vaccinium corymbosum var. bluecrop

Euphorbia characias L.: In Vitro Culture and the Production of Sterols, Triterpenols, and Hydrocarbon-Like Compounds

Contact Info

Product

Resources

About