Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax

Cunha, Ana; Fernandes-Ferreira, Manuel

doi:10.1016/s0168-9452(97)04587-1

Cited by 18 publications

(11 citation statements)

References 27 publications

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“…This decrease was related with calli formation (HSC) since calli cultures had a lower lipid content when compared to somatic embryos and hypocotyls ( Table 1). As already described for flax [22], the lipid content of calli was 2-3 times lower than that of somatic embryos. In both t 5 and t 7 sampling times no significant differences were detected in the total lipid content between embryogenic and non-embryogenic calli (Table 1).…”

Section: Lipid Content Changes In Flax Tissues During Somatic Embryogsupporting

confidence: 74%

Ontogenic variations in n-alkanes during somatic embryogenesis of flax (Linum usitatissimum L.)

Cunha

Fernandes-Ferreira

2001

Plant Science

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Hypocotyl segments (HS) of flax seedlings germinated in vitro, were used to induce indirect somatic embryogenesis on solid medium. The composition and distribution of n-alkanes in flax tissues collected at different developmental stages were studied by capillary gas chromatography (GC) and capillary gas chromatography-mass spectrometry (GC-MS). During induction and development of callus from hypocotyl tissues a decrease in the percentage of total lipids was observed. In all types of tissue sampled - HS used as primary explants, HS with differentiating calli at the cut ends (HSC), embryogenic (EC) and non-embryogenic calli (NEC) and somatic embryos (SE) - a skewed-normal distribution of n-alkanes with a low mass range (C(13)-C(21)) were found. The highest content of n-alkanes occurred in the primary hypocotyl explants and in the early stages of callus development. Longer carbon chain n-alkanes were observed only in the mature or differentiated tissues of hypocotyls and SE. Although the n-alkane contents decreased with time, in SE and calli, a significantly lower n-alkane content was observed in EC when compared to NEC independent of the time in culture. These results suggest the utilisation of n-alkanes for heterotrophic cellular growth as well as its mobilisation from EC to developing SE.

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Section: Lipid Content Changes In Flax Tissues During Somatic Embryogsupporting

confidence: 74%

Ontogenic variations in n-alkanes during somatic embryogenesis of flax (Linum usitatissimum L.)

Cunha

Fernandes-Ferreira

2001

Plant Science

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“…In addition the free sterol content variation during the process of somatic embryogenesis was reported (Cunha and Ferreira, 1997) and this work was extended to the determination of the esterified acids content (Cunha and Ferreira, 2003). Somatic embryos of flax were induced by an indirect method from callus by Tejavathi et al (2000), but growth progression of the shoot apex was not achieved.…”

Section: Somatic Embryogenesismentioning

confidence: 98%

Plant cell and biotechnology studies in Linum usitatissimum – a review

Millam¹,

Obert

Preťová

2005

Plant Cell Tiss Organ Cult

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The species Linum usitatissimum (flax/linseed) has been the focus of a great deal of both basic and applied research effort in plant cell and biotechnology studies in recent years. In this review we consider applications of the techniques of plant biotechnology in this species under several distinct headings. Plant cell and tissue regeneration strategies and applications are discussed, and the applications of the techniques of somatic embryogenesis, protoplast isolation, culture and fusion and cell suspension cultures in this species are described. A major area of study is the use of anther and microspore culture where clear advantages to breeding programmes could be applied. In addition, embryo and ovary culture studies have resulted in significant findings. The more recent technologies of gene transfer and expression by genetic transformation are reviewed, and a section on strategies for improvements in technological quality is also included. Finally we propose conclusions and future prospects for this ancient, but still highly relevant crop.

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“…As a result the ratio of stigmasterol/sitosterol increased. Such effect was also observed in regenerated shoots and somatic embryos of flax (Cunha and Ferreira 1997). As membrane permeability increases in the order: cholesterol < campesterol < sitosterol < stigmasterol (Grunwald 1975) observed changes suggest enhancement of membrane fluidity.…”

Section: Resultsmentioning

confidence: 60%

Changes in the composition of fatty acids and sterols of membrane lipids during induction and differentiation of Brassica napus (var. oleifera L.) callus

et al. 2002

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Changes in the membrane lipid and sterols content and composition were studied during induction and differentiation in callus cultures of Brassica napus var. oleifera. Callus induction was associated with an increase of DGDG content, significant changes in fatty acids composition of all lipid fractions and increased degree of lipid unsaturation. The membrane lipid composition of tissue at different degrees of differentiation was fbund to vary significantly, particularly two weeks after transfer of callus to regeneration medium. The main differences concerned the content and composition of galactolipids. Curiously in many cases, these differences declined during subsequent culture, in spite of the morphogenesis process which was in progress. Another result of differentiation was the change in free sterol composition: in shoot regenerating calli the content of stigmasterol had rose whereas the accumulation of campesterol decreased. Even though observed changes in membrane properties may not play a role in morphogenesis they are nevertheless useful as developmental markers and can be invaluable in understanding biochemical basis of morphogenesis.

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Differences in free sterols content and composition associated with somatic embryogenesis, shoot organogenesis and calli growth of flax

Cited by 18 publications

References 27 publications

Ontogenic variations in n-alkanes during somatic embryogenesis of flax (Linum usitatissimum L.)

Ontogenic variations in n-alkanes during somatic embryogenesis of flax (Linum usitatissimum L.)

Plant cell and biotechnology studies in Linum usitatissimum – a review

Changes in the composition of fatty acids and sterols of membrane lipids during induction and differentiation of Brassica napus (var. oleifera L.) callus

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