Hormonal Control of Lysosomal Enzymes During Spermatogenesis in the Rat1

Effect of prostaglandins A-1, E-2 and F-2 on spermatogenesis in rats

“…Males & Turkington (1971) observed that ß-glucuronidase activity is associated with the spermatogonia or Sertoli cells and that the level of this enzyme decreases during senescence. Any change in the number or function of spermatogonia or Sertoli cells would therefore be reflected in the activity of testicular ß-glucuronidase.…”

“…Hyaluronidase activity is particularly associated with spermatids in the testis (Steinberger & Nelson, 1955;Males & Turkington, 1970), the most marked decrease in enzyme levels occurring when spermatid formation and metamorphosis is impaired.…”

“…Levels of these enzymes can be maintained by FSH alone in hypophysectomized rats (Males & Turkington, 1970;Mills & Means, 1972). The formation of spermatids from spermatocytes is also regulated by FSH (Steinberger, 1971 …”

Lysosomal enzymes in cells separated from rat testis

“…Acid phosphatase is by far the most studied acid hydrolytic enzyme in the rat testis. Males & Turkington (1971) found by differential centrifugation of testis homogenates that the subcellular distribution of acid phosphatase and ß-glucuronidase is of lysosomal nature. Vanha-Perttula (1971) and Vanha-Perttula & Nikkanen (1973) (Parvinen & Vanha-Perttula, 1972).…”

“…Vanha-Perttula (1971) and Vanha-Perttula & Nikkanen (1973) (Parvinen & Vanha-Perttula, 1972). The cellular origin of acid phosphatase and other hydrolytic enzymes was also inferred from the changes of activity after suppression of germ cells in rat (Pecora & Arata, 1964;Ahlquist, 1966;Males & Turkington, 1971) and human (Guha & Vanha-Perttula, 1980) Steinberger et al 1975). The retained cells were rinsed three times with 10 ml KRBB (Fraction S); the filtered cell suspension, enriched with germinal cells, was centrifuged and the sediment was washed as described above (Fraction G).…”