Hormesis and debilitation effects in stress experiments using the nematode worm Caenorhabditis elegans: the model of balance between cell damage and HSP levels

Butov, A. A.; Johnson, Thomas E.; Cypser, James R.; Sannikov, Igor; Volkov, M. A.; Sehl, Mary E.; Yashin, Anatoli I.

doi:10.1016/s0531-5565(01)00161-9

Cited by 62 publications

(36 citation statements)

References 23 publications

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“…Modest environmental stressors have frequently been shown to enhance the average life span in a population (modeled in Mangel 2008;reviewed in Mattson 2008;see LeBourg and Rattan 2008;Love and Williams 2008b). Termed "hormesis" (Calabrese 2008), longevity has been enhanced by a range of stressors in, for example, mice given daily electric shocks (Ordy et al 1967), rats subjected to cold water immersion (Holloszy and Smith 1986), roundworm and fruit flies subjected to heat shock (Butov et al 2001;Cypser and Johnson 2002;Scannapieco et al 2007), and dietary or caloric restriction in a diversity of species (MacKay et al 1935;Weindruch and Walford 1988;Masoro et al 1991;Mair et al 2003). Our results may add to these reports in that mild stress exposure may contribute to increased life span in M-slow snakes.…”

Section: Environmental Stress Organismal Stress Response and Cellulmentioning

confidence: 59%

Evolution of Senescence in Nature: Physiological Evolution in Populations of Garter Snake with Divergent Life Histories

Robert

Bronikowski

2010

The American Naturalist

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Evolutionary theories of aging are linked to life-history theory in that age-specific schedules of reproduction and survival determine the trajectory of age-specific mutation/selection balances across the life span and thus the rate of senescence. This is predicted to manifest at the organismal level in the evolution of energy allocation strategies of investing in somatic maintenance and robust stress responses in less hazardous envirnments in exchange for energy spent on growth and reproduction. Here we report experiments from long-studied populations of western terrestrial garter snakes (Thamnophis elegans) that reside in low and high extrinsic mortality environments, with evolved long and short life spans, respectively. Laboratory common-environment colonies of these two ecotypes were tested for a suite of physiological traits after control and stressed gestations. In offspring derived from control and corticosteronetreated dams, we measured resting metabolism; mitochondrial oxygen consumption, ATP and free radical production rates; and erythrocyte DNA damage and repair ability. We evaluated whether these aging biomarkers mirrored the evolution of life span and whether they were sensitive to stress. Neonates from the long-lived ecotype (1) were smaller, (2) consumed equal amounts of oxygen when corrected for body mass, (3) had DNA that damaged more readily but repaired more efficiently, and (4) had more efficient mitochondria and more efficient cellular antioxidant defenses than short-lived snakes. Many ecotype differences were enhanced in offspring derived from stress-treated dams, which supports the conclusion that nongenetic maternal effects may further impact the cellular stress defenses of offspring. Our findings reveal that physiological evolution underpins reptilian life histories and sheds light on the connectedness between stress response and aging pathways in wild-dwelling organisms.

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Section: Environmental Stress Organismal Stress Response and Cellulmentioning

confidence: 59%

Evolution of Senescence in Nature: Physiological Evolution in Populations of Garter Snake with Divergent Life Histories

Robert

Bronikowski

2010

The American Naturalist

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“…It has been established that different concentrations of pharmacological agents can cause contradictory effects in nematodes, and may indicate that the compound acts upon multiple targets (Rand and Johnson, 1995). Also, a hormetic link between thermotolerance and longevity has been described in C. elegans (Butov et al, 2001;Cypser and Johnson, 2002). The fact that C. elegans daf-21/Hsp90 is known to be involved in thermotolerance and upregulated by DAF-16 (McElwee et al, 2003) further supports involvement of Hsp90 in a hormetic biological switch involved in stress responses.…”

Section: Discussionmentioning

confidence: 99%

Effects of Geldanamycin on Hatching and Juvenile Motility in Caenorhabditis elegans and Heterodera glycines

Skantar¹,

Agama

Meyer³

et al. 2005

J Chem Ecol

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Abstract-Several Streptomyces species are known to produce metabolites that inhibit plant pathogens. One such compound is geldanamycin (GA), a benzoquinone ansamycin originally isolated from Streptomyces hygroscopicus. We examined the effect of geldanamycin on egg hatch and juvenile motility in Caenorhabditis elegans and in two populations of the plant-parasitic nematode Heterodera glycines. When C. elegans eggs were exposed to geldanamycin, both hatch and motility were reduced by GA doses between 2 and 50 mg/ml. The H. glycines inbred populations TN17 and TN18 exhibited low dose stimulation of hatch and motility, whereas levels occurring at higher GA doses were at or below control levels. These experiments represent the first demonstration of geldanamycin effects in C. elegans and H. glycines and suggest that the heat shock chaperone Hsp90, the known molecular target of geldanamycin, may be involved in nematode egg hatch and motility. This study also indicates that geldanamycin-producing strains of Streptomyces may be useful as biocontrol agents for nematodes.

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“…This phenomenon is termed hormesis and has been shown to increase lifespan in several different species such as worms (Lithgow et al 1995a;Butov et al 2001;Cypser and Johnson 2001Yashin et al 2002;Rea et al 2005) and flies (Khazaeli et al 1997;Le Bourg et al 2000;Hercus et al 2003). Repeated hormetic heat treatments also delay the onset of several age-related changes observed in human fibroblasts in culture (Rattan 1998;Verbeke et al 2000Verbeke et al , 2001Verbeke et al , 2002Beedholm et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Lifespan extension of Caenorhabditis elegans following repeated mild hormetic heat treatments

2006

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Mild hormetic heat treatments early in life can significantly increase the lifespan of the nematode C. elegans. We have examined the effects of heat treatments at different ages and show that treatments early in life cause the largest increases in lifespan. We also find that repeated mild heat treatments throughout life have a larger effect on lifespan compared to a single mild heat treatment early in life. We hypothesize that the magnitude of the hormetic effect is related to the levels of heat shock protein expression. Following heat treatment young worms show a dramatic increase in the levels of the small heat shock protein HSP-16 whereas old worms are a 100-fold less responsive. The levels of the heat shock proteins HSP-4 and HSP-16 correlate well with the effects on lifespan by the hormetic treatments.

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Hormesis and debilitation effects in stress experiments using the nematode worm Caenorhabditis elegans: the model of balance between cell damage and HSP levels

Cited by 62 publications

References 23 publications

Evolution of Senescence in Nature: Physiological Evolution in Populations of Garter Snake with Divergent Life Histories

Evolution of Senescence in Nature: Physiological Evolution in Populations of Garter Snake with Divergent Life Histories

Effects of Geldanamycin on Hatching and Juvenile Motility in Caenorhabditis elegans and Heterodera glycines

Lifespan extension of Caenorhabditis elegans following repeated mild hormetic heat treatments

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