Effects of Geldanamycin on Hatching and Juvenile Motility in Caenorhabditis elegans and Heterodera glycines

Skantar, Andrea M.; Agama, Keli; Meyer, Seth D.; Carta, Lynn K.; Vinyard, Bryan T.

doi:10.1007/s10886-005-7114-z

Cited by 33 publications

(20 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Geldanamycin, unlike the other drugs in this study, was predicted to induce a transcriptional profile opposite to that of rapamycin. Another study showed that prolonged treatment of geldanamycin had detrimental effects on motility and is hence in line with our results (Skantar et al ., 2005). Geldanamycin inhibits Hsp90, mutants of which have been shown to have muscle cell defects (Gaiser et al ., 2011).…”

Section: Discussionmentioning

confidence: 99%

A network pharmacology approach reveals new candidate caloric restriction mimetics in C. elegans

et al. 2015

View full text Add to dashboard Cite

SummaryCaloric restriction (CR), a reduction in calorie intake without malnutrition, retards aging in several animal models from worms to mammals. Developing CR mimetics, compounds that reproduce the longevity benefits of CR without its side effects, is of widespread interest. Here, we employed the Connectivity Map to identify drugs with overlapping gene expression profiles with CR. Eleven statistically significant compounds were predicted as CR mimetics using this bioinformatics approach. We then tested rapamycin, allantoin, trichostatin A, LY‐294002 and geldanamycin in Caenorhabditis elegans. An increase in lifespan and healthspan was observed for all drugs except geldanamycin when fed to wild‐type worms, but no lifespan effects were observed in eat‐2 mutant worms, a genetic model of CR, suggesting that life‐extending effects may be acting via CR‐related mechanisms. We also treated daf‐16 worms with rapamycin, allantoin or trichostatin A, and a lifespan extension was observed, suggesting that these drugs act via DAF‐16‐independent mechanisms, as would be expected from CR mimetics. Supporting this idea, an analysis of predictive targets of the drugs extending lifespan indicates various genes within CR and longevity networks. We also assessed the transcriptional profile of worms treated with either rapamycin or allantoin and found that both drugs use several specific pathways that do not overlap, indicating different modes of action for each compound. The current work validates the capabilities of this bioinformatic drug repositioning method in the context of longevity and reveals new putative CR mimetics that warrant further studies.

show abstract

Section: Discussionmentioning

confidence: 99%

A network pharmacology approach reveals new candidate caloric restriction mimetics in C. elegans

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Carcasses and other debris were dissolved after 5-10 min, and resistant eggs (50% to 100% viable eggs) were collected and washed several times in an M-9 buffer [46]. The M-9 buffer was prepared with 1.5 g of KH 2 PO 4 , 3.0 g of Na 2 HPO 4 , 2.5 g of NaCl, 0.5 mL of 1 M MgSO 4 , and sterile distilled water to a final volume of 500 mL [68]. Eggs were deposit in agar plates for 48 h, and young worms (larval stage 4 and 5) were harvested and resuspended in the M-9 buffer.…”

Section: Toxicity Assaysmentioning

confidence: 99%

Use of Phycobiliproteins from Atacama Cyanobacteria as Food Colorants in a Dairy Beverage Prototype

Galetovic

Seura

Gallardo

et al. 2020

Foods

View full text Add to dashboard Cite

The interest of the food industry in replacing artificial dyes with natural pigments has grown recently. Cyanobacterial phycobiliproteins (PBPs), phycoerythrin (PE) and phycocyanin (PC), are colored water-soluble proteins that are used as natural pigments. Additionally, red PE and blue PC have antioxidant capabilities. We have formulated a new food prototype based on PBP-fortified skim milk. PBPs from Andean cyanobacteria were purified by ammonium sulfate precipitation, ion-exchange chromatography, and freeze-drying. The stability of PE and PC was evaluated by changes in their absorption spectra at various pH (1–14) and temperature (0–80 °C) values. Purified PBPs showed chemical stability under pH values of 5 to 8 and at temperatures between 0 and 50 °C. The antioxidant property of PBP was confirmed by ABTS (2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical ion scavenging, and FRAP (Ferric Antioxidant Power) assays. The absence of PBP toxicity against Caenorhabditis elegans was confirmed up to 1 mg PBP/mL. Skim milk fortified with PE obtained a higher score after sensory tests. Thus, a functional food based on skim milk-containing cyanobacterial PBPs can be considered an innovative beverage for the food industry. PBPs were stable at an ultra-high temperature (138 °C and 4 s). PBP stability improvements by changes at its primary structure and the incorporation of freeze-dried PBPs into sachets should be considered as alternatives for their future commercialization.

show abstract

“…The 24-well plates were covered with transparent plastic, and incubated at 20°C for 24 h. After incubation, nematodes were counted and determined as motile or non-motile using an inverted microscope. Nematodes were considered motile when they exhibited any movement, and as non-motile when there were no tail, head, or pharyngeal movements during 5 s of observation (Skantar et al 2005). It is important to differentiate motility in adult nematodes from movement caused by larvae hatched from eggs inside the body of dead C. elegans.…”

Section: Anthelmintic Assaymentioning

confidence: 99%

Effect of structurally related flavonoids from Zuccagnia punctata Cav. on Caenorhabditis elegans

D’Almeida

Arena

Morgan

et al. 2014

Acta Parasitologica

View full text Add to dashboard Cite

Zuccagnia punctata Cav. (Fabaceae), commonly called jarilla macho or pus-pus, is being used in traditional medicine as an antiseptic, anti-inflammatory and to relieve muscle and bone pain. The aim of this work was to study the anthelmintic effects of three structurally related flavonoids present in aerial parts of Z. punctata Cav. The biological activity of the flavonoids 7-hydroxyflavanone (HF), 3,7-dihydroxyflavone (DHF) and 2´,4´-dihydroxychalcone (DHC) was examined in the free-living nematode Caenorhabditis elegans. Our results showed that among the assayed flavonoids, only DHC showed an anthelmintic effect and alteration of egg hatching and larval development processes in C. elegans. DHC was able to kill 50% of adult nematodes at a concentration of 17 μg/mL. The effect on larval development was observed after 48 h in the presence of 25 and 50 μg/mL DHC, where 33.4 and 73.4% of nematodes remained in the L3 stage or younger. New therapeutic drugs with good efficacy against drug-resistant nematodes are urgently needed. Therefore, DHC, a natural compound present in Z. punctata, is proposed as a potential anthelmintic drug.

show abstract

Effects of Geldanamycin on Hatching and Juvenile Motility in Caenorhabditis elegans and Heterodera glycines

Cited by 33 publications

References 25 publications

A network pharmacology approach reveals new candidate caloric restriction mimetics in C. elegans

A network pharmacology approach reveals new candidate caloric restriction mimetics in C. elegans

Use of Phycobiliproteins from Atacama Cyanobacteria as Food Colorants in a Dairy Beverage Prototype

Effect of structurally related flavonoids from Zuccagnia punctata Cav. on Caenorhabditis elegans

Contact Info

Product

Resources

About