Horizontal transmission of the Picea glauca foliar endophyte Phialocephala scopiformis CBS 120377

Miller, J. David; Cherid, Hafsa; Sumarah, Mark W.; Adams, Gregory W.

doi:10.1016/j.funeco.2009.01.002

Cited by 29 publications

(21 citation statements)

References 24 publications

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“…Inoculated trees are more tolerant of the spruce budworm both in nurseries and under field conditions. The growth rate of the insect pest is reduced, exposing larvae to increased disease and predation by birds and reducing the damage to trees (3–5). In recent years, millions of conifer seedlings in New Brunswick production nurseries were inoculated with this strain to reduce the need for spraying chemical insecticides (6), and beneficial effects have persisted more than a decade postinoculation (7).…”

Section: Genome Announcementmentioning

confidence: 99%

Full Genome of Phialocephala scopiformis DAOMC 229536, a Fungal Endophyte of Spruce Producing the Potent Anti-Insectan Compound Rugulosin

et al. 2016

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Section: Genome Announcementmentioning

confidence: 99%

Full Genome of Phialocephala scopiformis DAOMC 229536, a Fungal Endophyte of Spruce Producing the Potent Anti-Insectan Compound Rugulosin

et al. 2016

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“…The usefulness of commercial enzyme‐linked immunosorbent assays (ELISAs) for detecting fungi and endophytic fungi in plants has been widely demonstrated (Miles et al ., ; Eibel et al ., ,; Fox & Narra, ; Miller et al ., ), and thus, ELISA represents a fast, sensitive and specific tool to detect infected plants. In addition, sequence analysis of coding regions, such as the CO1 gene, β‐tubulin gene, 18S, 28S and 5.8S genes of rDNA and noncoding internal transcribed spacer (ITS) regions of rDNA, has been successfully employed for fungal identification.…”

Section: Introductionmentioning

confidence: 99%

Polyclonal antibody‐based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants

Muniz

Freire

Viana

et al. 2012

Annals of Applied Biology

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Members of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, but for C. gloeosporioides, P. anacardii, P. guepinii and Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The feasibility of ELISA as an early detection technique to assist in gummosis management was demonstrated. PCR amplification based on ITS regions increases and complements serological specificity.

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“…To date ca. 80 of the 300 P. scopiformis -positive trees 89 have been sampled Miller et al 2009;Quiring & Miller, unpublished data). 90 The purpose of this paper is to describe the distribution of the fungus and its toxin throughout the 91 crown of one of the inoculated white spruce trees planted in 2003, now ~5m tall.…”

mentioning

confidence: 99%

Distribution of the foliar fungal endophyte Phialocephala scopiformis and its toxin in the crown of a mature white spruce tree as revealed by chemical and qPCR analyses

et al. 2014

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Horizontal transmission of the Picea glauca foliar endophyte Phialocephala scopiformis CBS 120377

Cited by 29 publications

References 24 publications

Full Genome of Phialocephala scopiformis DAOMC 229536, a Fungal Endophyte of Spruce Producing the Potent Anti-Insectan Compound Rugulosin

Full Genome of Phialocephala scopiformis DAOMC 229536, a Fungal Endophyte of Spruce Producing the Potent Anti-Insectan Compound Rugulosin

Polyclonal antibody‐based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants

Distribution of the foliar fungal endophyte Phialocephala scopiformis and its toxin in the crown of a mature white spruce tree as revealed by chemical and qPCR analyses

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