Hordein polymorphism in diploid and tetraploid Mediterranean populations of the Hordeum murinum L. complex

Amirouche, Rachid; Misset, Marie-Thérèse

doi:10.1007/s00606-003-0060-8

Cited by 13 publications

(8 citation statements)

References 35 publications

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“…Correlations between genetic variation in seed storage proteins and environmental factors have been also observed in wild barley (Hordeum spontaneum (C. Koch) Thell.) (Nevo et al 1983;Jaradat 1991;Amirouche and Misset 2003) and Lima beans (Vargas et al 2001). In contrast, little relationship between storage protein patterns and geographic origin was detected in a collection of Spanish bread wheat landraces (Ruiz et al 2002).…”

Section: Discussionmentioning

confidence: 97%

Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province

Liu

Zhang

et al. 2007

Genet Resour Crop Evol

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The high molecular weight (HMW) glutenin subunit composition of 111 common landraces of bread wheat collected from Hubei province, China has been determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Ninety six of the accessions were homogeneous for HMW glutenin subunit composition and 15 were heterogeneous. For the Glu-1 loci, 16 alleles were detected, 3 at the GluA1locus, 9 at the Glu-B1and 4 at the Glu-D1. Three novel alleles were identified, two at the Glu-B1 and one at the Glu-D1locus. Combination of these 16 alleles resulted in 14 different HMW subunit patterns. The distribution of HMW glutenin subunit alleles in a subset of 105 of the 111 accessions representing six populations was assessed both at the individual population and whole population levels. The results demonstrated that the distribution of allelic patterns varied among populations. Taken together, 62.5% of the alleles detected were considered to be rare alleles while the Glu-A1c (null), Glu-B1b (1Bx7 + 1By8) and Glu-D1a (1Dx2 + 1Dy12) alleles were found most frequently in the six populations. The subset exhibited relatively high genetic diversity (A = 5.33, P = 1.00, Ae = 1.352 and He = 0.238) with 81.5% of the diversity being within populations and 18.5% between populations.

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Section: Discussionmentioning

confidence: 97%

Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province

Liu

Zhang

et al. 2007

Genet Resour Crop Evol

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“…leporinum and French subsp. murinum populations also showed no signifi cant differentiation between the two 4x subspecies (AMIROUCHE and MISSET 2003). Therefore, additional studies of the two tetraploid subspecies are needed, including DNA sequence analysis of additional loci and analysis of more tetraploid accessions.…”

Section: Discussionmentioning

confidence: 99%

Analysis of DNA sequence polymorphism at the cMWG699 locus reveals phylogenetic relationships and allopolyploidy withinHordeum murinumsubspecies

et al. 2010

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Hordeum murinum L. is one of the most widely distributed species in the genus Hordeum. This species is composed of three subspecies with three ploidy levels, namely subsp. glaucum (2x=14), subsp. murinum (4x=28) and subsp. leporinum (4x=28, 6x=42). These three subspecies are morphologically similar and are frequently referred to as the 'murinum complex'. Although many cytological studies suggest that the murinum complex is allopolyploid, one inter-specific hybridization study suggested that it is autopolyploid. The goals of the present study are to identify nucleotide variation in the cMWG699 locus in the polyploid genomes of the murinum complex, to conduct molecular phylogenetic analysis of this locus, and to clarify the allo- versus auto-polyploidy status of the murinum complex. For this purpose, PCR-RFLP analysis was conducted with HhaI and SspI restriction enzymes on 80 H. murinum accessions. Single enzyme digestion data revealed polymorphism between diploid and polyploids, and double-digestion revealed polymorphism between tetra- and hexaploids. The nucleotide sequences of clones clearly show that polyploid murinum species are allopolyploid. In addition, DNA sequence analysis indicated that one donor of the tetraploid was subsp. glaucum (2x), as has been suggested previously by cytological studies. The other diploid donors were not identified, but at least one group of sequences common to 4x and 6x genomes (namely clonetype B) was highly diverged from 2x subsp. glaucum. The two tetraploid subspecies, 4x subsp. murinum and 4x subsp. leporinum, had identical DNA sequences, suggesting that these two subspecies are not differentiated at the cMWG699 locus.

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“…During meiosis the polyploids of H. murinum behave like allopolyploids, which means that chromosomes exclusively form bivalents. Also protein electrophoresis (Booth and Richards, 1978;Jaaska, 1992;Amirouche and Misset, 2003) and karyological analyses (e.g., Linde-Laursen et al, 1989;Taketa et al, 2000) indicate an allopolyploid origin of subsp. leporinum and murinum with subsp.…”

Section: Introductionmentioning

confidence: 99%

Two extinct diploid progenitors were involved in allopolyploid formation in the Hordeum murinum (Poaceae: Triticeae) taxon complex

Jakob

Blattner

2010

Molecular Phylogenetics and Evolution

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Hordein polymorphism in diploid and tetraploid Mediterranean populations of the Hordeum murinum L. complex

Cited by 13 publications

References 35 publications

Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province

Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province

Analysis of DNA sequence polymorphism at the cMWG699 locus reveals phylogenetic relationships and allopolyploidy withinHordeum murinumsubspecies

Two extinct diploid progenitors were involved in allopolyploid formation in the Hordeum murinum (Poaceae: Triticeae) taxon complex

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