Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province

Abstract

The high molecular weight (HMW) glutenin subunit composition of 111 common landraces of bread wheat collected from Hubei province, China has been determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Ninety six of the accessions were homogeneous for HMW glutenin subunit composition and 15 were heterogeneous. For the Glu-1 loci, 16 alleles were detected, 3 at the GluA1locus, 9 at the Glu-B1and 4 at the Glu-D1. Three novel alleles were identified, two at the Glu-B1 and one at the Glu-D1locus. Combination of these 16 alleles resulted in 14 different HMW subunit patterns. The distribution of HMW glutenin subunit alleles in a subset of 105 of the 111 accessions representing six populations was assessed both at the individual population and whole population levels. The results demonstrated that the distribution of allelic patterns varied among populations. Taken together, 62.5% of the alleles detected were considered to be rare alleles while the Glu-A1c (null), Glu-B1b (1Bx7 + 1By8) and Glu-D1a (1Dx2 + 1Dy12) alleles were found most frequently in the six populations. The subset exhibited relatively high genetic diversity (A = 5.33, P = 1.00, Ae = 1.352 and He = 0.238) with 81.5% of the diversity being within populations and 18.5% between populations.

Genetic divergence for high-molecular weight glutenin subunits (HMW-GS) in indigenous landraces and commercial cultivars of bread wheat of Pakistan

“…A large number of rare alleles was reported, which were confirmed by the findings of Branlard (2003), Tabasum (2011), andDessalegn (2011). Several novel HMW-GS alleles, such as 7*, 7**, 8*, and 8** (Liu et al, 2007), were detected in the present study and have also been detected in Chinese wheat landraces.…”

“…Because most wheat breeding programs used 5+10, subunits 17+18, 13+16, and 14+15 should also be used, as they have a positive influence on the grain quality indices of wheat. As a result, genetic diversity as well as end-use quality would improve (Liu et al, 2007). The exploration of diversity in available genetic stocks and quality associated with favorable alleles is important, and can be effectively utilized to develop new cultivars and improve crops.…”

Allelic variation and genetic diversity of high molecular weight glutenin subunit in Chinese endemic wheats (Triticum aestivum L.)

“…All accessions were obtained from the Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing. Chinese Spring, ZM11217, ZM11221, and 12 European bread wheat and durum cultivars from Rothamsted Research Harpenden, UK, were used as standards to identify HMW-GS alleles (Liu et al 2007). …”

“…Methods for seed protein extraction and SDS-PAGE were as described by Liu et al (2007). All accessions were initially analysed using five randomly selected seeds with an additional 20 seeds being analysed for those found to be heterogeneous.…”

10.5937/ratpov50-5098 = High molecular weight (HMW) glutenin subunit composition of NS wheat cultivars released in 1987-2008

“…2). After various authors (Deng et al 2005, Liu et al 2007, Rhazi et al 2009) both of the mentioned GS at the locus Glu-B1 have positive effect, while GS 4+12 has negative effect on the breadmaking quality. Bekes et al (2008) states that GS 14+15 at the locus Glu-B1 is predominant in the Swedish cultivars but rare in the Finnish ones, which suggests possible northern origin.…”