Hop stunt viroid: A polyphagous pathogenic RNA that has shed light on viroid–host interactions

Marquez‐Molins, Joan; Gómez, Gustavo; Pallás, Vicente

doi:10.1111/mpp.13022

Cited by 25 publications

(22 citation statements)

References 106 publications

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“…Among all known viroids, HSVd has the widest range of host plants [124][125][126]. To date, the GenBank contains HSVd sequences found on hop, wild apple tree, apricot, sweet cherry, sour cherry, hibiscus, cucumber, grapevine, citrus fruits, plum, and peach [127].…”

Section: Viroidsmentioning

confidence: 99%

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Navrotskaya

Porotikova

Yurchenko

et al. 2021

Viruses

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The use of high-throughput sequencing (HTS) technology has led to significant progress in the identification of many viruses and their genetic variants. In this study, we used the HTS platform to sequence small RNAs (sRNAs) of grapevine to study the virome. Isolation of RNA was performed using symptomatic grapevines collected from commercial vineyards in Krasnodar Krai in 2017–2018. To determine the viromes of vineyards, we used an integrated approach that included a bioinformatic analysis of the results of sRNA HTS and the molecular method RT-PCR, which made it possible to identify 13 viruses and 4 viroids. Grapevine leafroll-associated virus 4 (GLRaV-4), Grapevine Syrah Virus-1 (GSyV-1), Raspberry bushy dwarf virus (RBDV), Australian grapevine viroid (AGVd), and Grapevine yellow speckle viroid 2 (GYSVd-2) were identified for the first time in Russia. Out of 38 samples analyzed, 37 had mixed infections with 4–11 viruses, indicating a high viral load. Analysis of the obtained sequences of fragments of virus genomes made it possible to identify recombination events in GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-4, GVT, GPGV, GRSPaV, GVA, and GFLV. The obtained results indicate a wide spread of the viruses and a high genetic diversity in the vineyards of Krasnodar Krai and emphasize the urgent need to develop and implement long-term strategies for the control of viral grapevine diseases.

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Section: Viroidsmentioning

confidence: 99%

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Navrotskaya

Porotikova

Yurchenko

et al. 2021

Viruses

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“…In viroids, the mature RNA form predominantly accumulated in the host is referred to as the plus strand. HSVd has been found inducing symptomatic infection in a wide range of hosts and has been frequently used to explore diverse aspects of viroid-host interactions [30][31][32][33]. ELVd induces asymptomatic infections and is assumed as a valuable model to study molecular aspects of the family Avsunviroidae [34].…”

Section: Hsvd and Elvd Contains Putative Orfs Able To Encode Peptidesmentioning

confidence: 99%

Might exogenous circular RNAs act as protein-coding transcripts in plants?

et al. 2021

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Circular RNAs (circRNAs) are regulatory molecules involved in the modulation of gene expression. Although originally assumed as non-coding RNAs, recent studies have evidenced that animal circRNAs can act as translatable transcripts. The study of plant-circRNAs is incipient, and no autonomous coding plant-circRNA has been described yet. Viroids are the smallest plant-pathogenic circRNAs known to date. Since their discovery 50 years ago, viroids have been considered valuable systems for the study of the structure-function relationships in RNA, essentially because they have not been shown to have coding capacity. We used two pathogenic circRNAs (Hop stunt viroid and Eggplant latent viroid) as experimental tools to explore the coding potential of plant-circRNAs. Our work supports that the analysed viroids contain putative ORFs able to encode peptides carrying subcellular localization signals coincident with the corresponding replicationspecific organelle. Bioassays in well-established hosts revealed that mutations in these ORFs diminish their biological efficiency. Interestingly, circular forms of HSVd and ELVd were found to co-sediment with polysomes, revealing their physical interaction with the translational machinery of the plant cell. Based on this evidence we hypothesize about the possibility that plant circRNAs in general, and viroids in particular, can act, under certain cellular conditions, as non-canonical translatable transcripts.

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“…Em citros, causa a doença conhecida por xiloporose (EIRAS et al 2009). O HSVd pode ser transmitido mecanicamente por meio de ferramentas de corte empregadas em cultivos que requerem enxertia e outros tipos de tratos culturais como poda e desbaste, e por sementes verdadeiras (SINGH et al 2003b;MARQUEZ-MOLINS et al 2021).…”

Section: Hop Stunt Viroid (Hsvd)unclassified

Pospiviroides de importância para hortaliças e ornamentais: panorama dos aspectos fitopatológicos e dos riscos para os sistemas agrícolas brasileiros

Wierzbicki¹,

Eiras²

2021

RAPP

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Há cerca de 50 anos, a etiologia de uma série de doenças de plantas, até então desconhecidas ou atribuídas a possíveis vírus, começou a ser elucidada com a descoberta dos viroides. Minúsculos agentes fitopatogênicos e constituídos exclusivamente por uma molécula de RNA circular de fita simples, os viroides têm causado epidemias, principalmente em cultivos protegidos de hortaliças e ornamentais. Apesar de induzirem sintomas que podem ser severos, muitas infecções transcorrem sem que haja a expressão de sintomas perceptíveis, dificultando o diagnóstico, o que possibilita a perpetuação dos viroides nos cultivos, facilitando a sua disseminação. Atualmente, as taxas de transmissão de viroides por sementes e sua distribuição geográfica incerta e dinâmica têm suscitado questões relacionadas à produção de sementes e aos riscos de disseminação desses patógenos. Pretende-se com esta revisão abordar e discutir aspectos da biologia dos pospiviroides (família Pospiviroidae), especificamente dos gêneros Pospiviroid, Hostuviroid e Coleviroid, que infectam hortaliças e ornamentais, incluindo o estado da arte da sua distribuição geográfica e ocorrência, classificação, sintomas, transmissão, diagnóstico, epidemiologia e estratégias para o manejo e controle desses patógenos

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Hop stunt viroid: A polyphagous pathogenic RNA that has shed light on viroid–host interactions

Cited by 25 publications

References 106 publications

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Might exogenous circular RNAs act as protein-coding transcripts in plants?

Pospiviroides de importância para hortaliças e ornamentais: panorama dos aspectos fitopatológicos e dos riscos para os sistemas agrícolas brasileiros

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