Honeybush (Cyclopia spp.) pollen viability and surface morphology

Koen, J.; Slabbert, M.M.; Booyse, M.; Bester, C.

doi:10.1016/j.sajb.2019.11.004

Cited by 8 publications

(2 citation statements)

References 32 publications

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“…Volgens Penfield en MacGregor (2016), kan selfs geringe verskille in moederplant en/of omgewingsfaktore tot saaddimorfisme aanleiding gee, omdat die saadhuid 'n "hoogs plastiese orgaan" is, m.a.w baie reaktief op omgewingsseine (ongeag of dit deur die moedergenotipe of 'n direkte reaksie op die sigoot is). Onlangse studies op heuningbosspesies het sommige van hierdie raaisels begin ontrafel betreffende die blomstruktuur en stuifmeelkiemkragtigheid, wat ook 'n effek op die interaksies tussen bestuiwing en gevolglik suksesvolle bestuiwing kan hê (Koen et al, 2020a;Koen et al, 2020b)…”

Section: Effek Van Saadposisieunclassified

Honeybush ( Cyclopia spp.) mature embryo culture and the influence of pod position on seed colour

Koen¹,

Slabbert

Booyse³

et al. 2021

Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie

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Plante (verskillende genotipes) van Cyclopia spesies word gebruik om Suid-Afrikaanse heuningbostee (’n kruietee produk) te produseer en word tans deur teling vir grootskaalse verbouing verbeter. Saaddimorfisme en dormansie beïnvloed egter die ontkiemingspersentasie negatief. Deur gebruik te maak van volwasse embrioweefselkulture is studies om die ontkiemingspersentasie van saad te verhoog op C. longifolia, C. maculata en C. subternata gedoen. Die effek van stratifikasie op saadontkieming deur geen of ’n kouebehandeling toe te pas, is ook ondersoek. Volwasse embrios is hierna uit sade verwyder en vir ’n 24-uur fotoperiode by 25°C ± 1°C op weefselkultuur gekweek. Die kweek van volwasse embrios deur middel van weefselkulture was suksesvol en het deurlopend meer plante vergeleke met konvensionele saaimetode gelewer. Studies om die effek wat saadpeulposisie van die moederplant op saadkleur het, is ook gedoen. Volwasse heuningbospeule is vanaf die boonste, middelste en onderste-derdes van die blaarbedekte deel van die heuningbosplante versamel om die aantal groen/intermediêre/bruin sade wat by elke gedeelte geproduseer is, te bepaal. Die verhouding van saaddimorfisme wat by elke gedeelte geproduseer word, kon egter nie deur peulposisie voorspel word nie.

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Section: Effek Van Saadposisieunclassified

Honeybush ( Cyclopia spp.) mature embryo culture and the influence of pod position on seed colour

Koen¹,

Slabbert

Booyse³

et al. 2021

Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie

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“…A review of the literature regarding viability of stored pollen indicates that there is a great degree of variability in the response of pollen grains of individual plant species, or even a variety or cultivar, to storage temperature treatments (Almeida, Saziki, & Cardoso, 2019). This has been partially attributed to the temperature‐dependent rates of pollen metabolic activities (Du et al, 2019), the size of the pollen grains (García Cruzatty, Rivero, & Droppelmann, 2015; Koen, Slabbert, Booyse, & Bester, 2020) or the plant genetic background (Beltrán et al, 2019). On the other hand, availability of a successful protocol for storage of viable pollen of L. rosea can be used to overcome the deleterious effects of abiotic stresses that can occur at the blossom stage, hindering the pollen viability inside the anthers (Dolferus & Pacini, 2019).…”

Section: Introductionmentioning

confidence: 99%

New protocol for storage of viable pollen of Lapageria rosea (Philesiaceae), an endangered plant species endemic to temperate forests of Chile

Cruzatty

Droppelmann

Izaguirre‐Mayoral

2020

Plant Species Biology

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Lapageria rosea is an emblematic species, endemic to the temperate rainforest of Chile. As a result of extensive plantation of commercial exotic pine and eucalypts, scattered individuals of L. rosea are now encountered mainly in fragmented forest areas. Therefore, L. rosea has been declared in danger of extinction. However, the success of national conservation programs depends, in part, on the availability of stored viable pollen grains for pollinating assays, in order to sustain the genetic diversity of L. rosea offspring. Here we describe a simple and low-cost protocol for storing viable pollen grains for 1 year. The protocol involves oven-drying freshly collected pollen grains at 37 C for 15 min, followed by storage at-13 C. The stored pollen has an average of 60-65% in vitro germination after 5 months, with a reduction to 50% by the end of

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Effects of pulsed vacuum drying temperature on drying kinetics, physicochemical properties and microstructure of bee pollen

Wang¹,

Bi²,

Zhou³

et al. 2022

LWT

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Honeybush (Cyclopia spp.) pollen viability and surface morphology

Cited by 8 publications

References 32 publications

Honeybush ( Cyclopia spp.) mature embryo culture and the influence of pod position on seed colour

Honeybush ( Cyclopia spp.) mature embryo culture and the influence of pod position on seed colour

New protocol for storage of viable pollen of Lapageria rosea (Philesiaceae), an endangered plant species endemic to temperate forests of Chile

Effects of pulsed vacuum drying temperature on drying kinetics, physicochemical properties and microstructure of bee pollen

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