Honeybee (Apis mellifera) resistance to deltamethrin exposure by Modulating the gut microbiota and improving immunity

Dong, Zhi-Xiang; Tang, Qihe; Li, Wan-LI.; Wang, Zheng-Wei; Li, Xi-Jie; Fu, Chao-Min; Li, Dan; Qian, Kai; Tian, Wenli; Guo, Jun

doi:10.1016/j.envpol.2022.120340

Cited by 15 publications

(13 citation statements)

References 39 publications

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“…Honeybee gut microbes have a variety of known and unknown functions, and may also have the ability to protect the host against the effects of exogenous toxic substances. Currently, Apilactobacillus kunkeei (original classification as Lactobacillus kunkeei) can help to increase the resistance of the host to deltamethrin, as confirmed in our previous studies (Dong et al, 2022). However, which gut bacteria can protect honeybees from toxic plants remains unknown.…”

Section: Introductionmentioning

confidence: 71%

“…Lactobacillus is a well‐known probiotic, and A. kunkeei is common in the honey bee gut and colony environment and has been shown to produce bacteriocins that are better inhibitors of pathogenic bacteria (Dyrhage et al, 2022; Ebrahimi et al, 2021). In our previous study, we found that deltamethrin exposure increased the abundance of A. kunkeei and that it could help bees resist the effects of deltamethrin by modulating honey bee immunity (Dong et al, 2022). Here, we also found that exposure to A. kunkeei increased the abundance of Lactobacillus and that supplementation with A. kunkeei or B. apis enhanced the tolerance of honeybees to B. pilosa and further improved the immune genes of honeybees.…”

Section: Discussionmentioning

confidence: 99%

“…The number of dead bees in each group was recorded every 24 h and the syrup was replaced until day 7. The source of the strains was described in our previous study (Dong et al, 2022).…”

Section: Gf Honeybee Experimentsmentioning

confidence: 99%

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Gut microbiome helps honeybee (Apis mellifera) resist the stress of toxic nectar plant (Bidens pilosa) exposure: Evidence for survival and immunity

Tang

Wang

et al. 2023

Environmental Microbiology

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Honeybee (Apis mellifera) ingestion of toxic nectar plants can threaten their health and survival. However, little is known about how to help honeybees mitigate the effects of toxic nectar plant poisoning. We exposed honeybees to different concentrations of Bidens pilosa flower extracts and found that B. pilosa exposure significantly reduced honeybee survival in a dose‐dependent manner. By measuring changes in detoxification and antioxidant enzymes and the gut microbiome, we found that superoxide dismutase, glutathione‐S‐transferase and carboxylesterase activities were significantly activated with increasing concentrations of B. pilosa and that different concentrations of B. pilosa exposure changed the structure of the honeybee gut microbiome, causing a significant reduction in the abundance of Bartonella (p < 0.001) and an increase in Lactobacillus. Importantly, by using Germ‐Free bees, we found that colonization by the gut microbes Bartonella apis and Apilactobacillus kunkeei (original classification as Lactobacillus kunkeei) significantly increased the resistance of honeybees to B. pilosa and significantly upregulated bee‐associated immune genes. These results suggest that honeybee detoxification systems possess a level of resistance to the toxic nectar plant B. pilosa and that the gut microbes B. apis and A. kunkeei may augment resistance to B. pilosa stress by improving host immunity.

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Section: Introductionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

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Gut microbiome helps honeybee (Apis mellifera) resist the stress of toxic nectar plant (Bidens pilosa) exposure: Evidence for survival and immunity

Tang

Wang

et al. 2023

Environmental Microbiology

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“…Insect pollination is an important contributor to our ecosystem and biodiversity 1 . Because of various factors, the populations of pollinating insects, especially bees, have become significantly reduced 1,2 . The most important of these factors are abiotic stressors, such as environmental pollution and climate change 2 .…”

Section: Introductionmentioning

confidence: 99%

“…1 Because of various factors, the populations of pollinating insects, especially bees, have become significantly reduced. 1,2 The most important of these factors are abiotic stressors, such as environmental pollution and climate change. 2 Apis cerana cerana, a subspecies of the Asian honeybee, is widely cultivated owing to its strong stress resistance and high collection efficiency.…”

Section: Introductionmentioning

confidence: 99%

The gene AccCyclin H mitigates oxidative stress by influencing trehalose metabolism in Apis cerana cerana

Peng,

Guo,

Peng

et al. 2023

J Sci Food Agric

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BACKGROUNDEnvironmental stress can induce oxidative stress in A. cerana cerana, leading to cellular oxidative damage, reduced vitality, and even death. Currently, due to an incomplete understanding of the molecular mechanisms by which A. cerana cerana resists oxidative damage, there is no available method to mitigate the risk of this type of damage. Cyclin plays an important role in cell stress resistance. The aim of this study was to explore the in vivo protection of cyclin H against oxidative damage induced by abiotic stress in A. cerana cerana and clarify the mechanism of action. We isolated and identified the AccCyclin H gene in A. cerana cerana and analysed its responses to different exogenous stresses.RESULTSThe results showed that different oxidative stressors can induce or inhibit the expression of AccCyclin H. After RNAi‐mediated AccCyclin H silencing, the activity of antioxidant‐related genes and related enzymes was inhibited, and trehalose metabolism was reduced. AccCyclin H gene silencing reduced A. cerana cerana high‐temperature tolerance. Exogenous trehalose supplementation enhanced the total antioxidant capacity of A. cerana cerana, reduced the accumulation of oxidants, and improved the viability of A. cerana cerana under high‐temperature stress.CONCLUSIONOur findings suggest that trehalose can alleviate adverse stress and that AccCyclin H may participate in oxidative stress reactions by regulating trehalose metabolism.This article is protected by copyright. All rights reserved.

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Toxic effects of acaricide fenazaquin on development, hemolymph metabolome, and gut microbiome of honeybee (Apis mellifera) larvae

Wu,

Liu,

Sun

et al. 2024

Chemosphere

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Honeybee (Apis mellifera) resistance to deltamethrin exposure by Modulating the gut microbiota and improving immunity

Cited by 15 publications

References 39 publications

Gut microbiome helps honeybee (Apis mellifera) resist the stress of toxic nectar plant (Bidens pilosa) exposure: Evidence for survival and immunity

Gut microbiome helps honeybee (Apis mellifera) resist the stress of toxic nectar plant (Bidens pilosa) exposure: Evidence for survival and immunity

The gene AccCyclin H mitigates oxidative stress by influencing trehalose metabolism in Apis cerana cerana

Toxic effects of acaricide fenazaquin on development, hemolymph metabolome, and gut microbiome of honeybee (Apis mellifera) larvae

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