Honey as an apitherapic product: its inhibitory effect on urease and xanthine oxidase

Abstract

The aim of this study was to evaluate new natural inhibitor sources for the enzymes urease and xanthine oxidase (XO). Chestnut, oak and polyfloral honey extracts were used to determine inhibition effects of both enzymes. In addition to investigate inhibition, the antioxidant capacities of these honeys were determined using total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity assays. Due to their high phenolic content, chestnut and oak honeys are found to be a powerful source for inhibition of both enzymes. Especially, oak honeys were efficient for urease inhibition with 0.012-0.021 g/mL IC 50 values, and also chestnut honeys were powerful for XO inhibition with 0.028-0.039 g/mL IC 50 values. Regular daily consumption of these honeys can prevent gastric ulcers deriving from Helicobacter pylori and pathological disorders mediated by reactive oxygen species.

Effect of propolis in gastric disorders: inhibition studies on the growth ofHelicobacter pyloriand production of its urease

“…Similarly, the P11 was distinguished from the other samples as it showed the greatest degree of urease inhibition. Similar to our results, a number of natural compounds, rich in polyphenolic agents, such as caffeic acid 28,29 , rutin 30 , as well as chestnut and oak honeys 13 and honey fractions 11 have been reported to inhibit H. pylori urease.…”

“…Therefore, the Eregli and Balikesir propolis were distinguished from the other propolis samples. It has been reported that the propolis from both of these areas could be derived from chestnut flora 12 , and chestnut honey and pollen samples have high levels of phenolic substances 13 . The total flavonoid content of the propolis samples ranged from 3.882 to 12.453 mg Q/g sample (Table 1).…”

“…Honey, pollen and propolis are bee products that have been used in traditional and complementary medicine since ancient times 13 . Propolis is a resinous mixture used to protect hives from many diseases and threats.…”

Properties of Antioxidant and Anti-Inflammatory Activity and Phenolic Profiles of Şevketi Bostan (Cnicus benedictus L.) Cultivated in Aegean Region from Turkey

“…XO is responsible for the oxidative damage responsible for several pathological diseases, including gout, hyperuricemia, hepatitis and aging (Mehta et al, 2014). Therefore, inhibiting this enzyme is important for the prevention of many inflammatory diseases (Sahin, 2015).Urease catalyses the degradation of urea in many physiological processes, and inhibiting urease is particularly important in the treatment of gastric diseases caused by Helicobacter pylori Ndemangou et al, 2013).…”

Inhibition of Helicobacter pylori and Its Associated Urease by Two Regional Plants of San Luis Argentina

“…Is well accepted that urease-negative mutant does not cause gastritis due to difficulties in colonization, therefore, specific inhibition of urease activity could be a possible strategy to eliminate the microorganism. In this sense, several authors have studied the inhibitory effect of plant extracts on urease activity (Amin et al, 2013;Sahin, 2015, Sarkar et al, 2016Zhou et al, 2016).…”