Homozygosity of the Pro12Ala variant of the peroxisome proliferation-activated receptor-γ2 (PPAR-γ2): divergent modulating effects on body mass index in obese and lean Caucasian men

Influence of Pro12Ala peroxisome proliferator-activated receptor γ2 polymorphism on glucose response to exercise training in type 2 diabetes

“…This Pro 12 Ala SNP has been associated with reduced transcriptional activity in vitro [17]. Discordant results have been reported in the literature [17][18][19][20][21][22][23]; however, a recent meta-analyses of 16 studies, which included over 3,000 individuals, demonstrated that the Ala allele confers protection from diabetes (21% risk reduction) [24,25]. It is possible that the environment, particularly diet or exercise habits, could modify the genotype effect and may account for a portion of the disparity found between separate studies.…”

Observation�Effect of the Pro12Ala polymorphism of the PPAR-?2 gene on long-term weight change in Finnish non-diabetic subjects

“…This agrees with the previous study, in which the Ala12Ala genotype was associated with a lower BMI among lean subjects (BMI < 25 kg/m 2 at draft board) but Letters 925 with a higher BMI among obese subjects (BMI > 30 kg/m 2 at draft board) during 24 years' follow-up [6]. In our study, subjects with the Ala12Ala genotype had lower fasting and 2-h plasma insulin concentrations at the baseline and also at the 10-year examination despite their greater weight gain, compared to those with other genotypes, which indicates a better insulin sensitivity in subjects with the Ala12Ala genotype.…”

Association between the Pro12Ala variant of the peroxisome proliferator-activated receptor-gamma2 gene and increased 24-h diastolic blood pressure in obese patients with type II diabetes

“…The allele frequencies and their 95% confidence intervals (95%CI) were 85.5% (95% CI: 81.9-88.5%) Pro and 14.5% (95% CI: 11.5-18.1%) Ala. For the whole studied group, the distribution of PPAR-g2 genotypes and allele frequencies in our study were similar to previously published data. 6,7,10 However, analysis of the Pro12Ala polymorphism gender distribution demonstrated that Ala allele was significantly more frequent in male than in female individuals (P ¼ 0.048) ( Table 1).…”

“…The Pro12Ala PPAR-g2 variant was analysed in all the patients by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method described by Ek et al 10 Genomic DNA was isolated from peripheral blood leukocytes with nonenzymatic and nonorganic method. 11 The following pair of primers was used for DNA amplification by PCR: 5 0 -CAAGCCCAGTCCTTTCTGTG-3 0 as a sense primer (PPAR-f) and 5 0 -AGTGAAGGAATC GCTTTCCG-3 0 as an antisense primer (PPAR-r) (GenBank accession AB005520).…”