Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids

Eghrari, Kian; Brito, André Humberto de; Baldassi, Amanda Cristina; Balbuena, Tiago Santana; Môro, Gustavo Vitti

doi:10.1016/j.cropro.2019.104871

Cited by 12 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In other words, no signal could be detected relating possible gene-dosage effects to Cry1Ab concentration. This finding stands in contrast with previous reports suggesting that homozygous GM maize hybrids had increased expression levels of Bt proteins and increased S. frugiperda control [76].…”

Section: Cry1ab Concentrationcontrasting

confidence: 99%

See 1 more Smart Citation

Transgene behavior in Zea mays L. crosses across different genetic backgrounds: Segregation patterns, cry1Ab transgene expression, insecticidal protein concentration and bioactivity against insect pests

et al. 2020

View full text Add to dashboard Cite

Brazil and South Africa, countries with economies in transition, are characterized by a dual agrarian structure co-occurring, sometimes, alongside in the same region. Large-scale commercial farming produces crops for export to global markets on the one hand, and smallscale farming, on the other hand, provides for subsistence and local markets. In both systems, maize (Zea mays) is a key crop for these two countries. For the commercial system, maize is a commodity crop while for the small-scale system it is the prime staple crop. In commercial systems, farmers predominantly grow genetically modified (GM) hybrid maize. In small-scale systems, farmers rely on open pollinated varieties (OPVs) and/or landraces and are largely dependent on seed saving systems. The aim of this study was to understand the relationship between transgene expression rates, the resulting concentrations of the transgene product (Bt protein) and its bioactivity in insect pests following transgene flow from GM hybrid maize into non-genetically modified, non-GM near-isogenic maize hybrid (ISO) and OPVs. We modeled segregation patterns and measured cry1Ab transgene expression (mRNA quantification), Cry1Ab protein concentration and pest survival. Two groups of F1, F2 crosses and backcrosses with GM, ISO and OPV maize varieties from Brazil and South Africa were used. Bioassays with the larvae of two lepidopteran maize pest species, Helicoverpa armigera and Spodoptera littoralis, were carried out. Overall, the cry1Ab transgene outcrossed effectively into the genetic backgrounds tested. The cry1Ab transgene was stably expressed in both ISO and OPV genetic backgrounds. Transgene introgression led to consistent, though highly variable, concentrations of Cry1Ab toxins that were similar to those observed in GM parental maize. Most crosses, but not all, suggested the expected Mendelian segregation pattern. Transgene expression rates were significantly higher than expected from purely Mendelian segregation in the South African crosses. In South African materials, ISO and OPV crosses produced significantly lower Cry1Ab

show abstract

Section: Cry1ab Concentrationcontrasting

confidence: 99%

“…This finding stands in contrast with previous reports suggesting that homozygous GM maize hybrids had increased expression levels of Bt proteins and increased S . frugiperda control [ 76 ].…”

Section: Resultsmentioning

confidence: 99%

Transgene behavior in Zea mays L. crosses across different genetic backgrounds: Segregation patterns, cry1Ab transgene expression, insecticidal protein concentration and bioactivity against insect pests

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is an important insect pest worldwide. The insect can feed on at least 353 plant species including major crops such as corn, rice, soybeans, sugar cane, and cotton [1][2][3]. S. frugiperda is native to tropical and subtropical regions of the Americas, but has been spread to Africa and Asia in recent years [4].…”

Section: Introductionmentioning

confidence: 99%

Growth inhibition of Spodoptera frugiperda larvae by camptothecin correlates with alteration of the structures and gene expression profiles of the midgut

Shu

Zou

et al. 2021

BMC Genomics

View full text Add to dashboard Cite

Background Spodoptera frugiperda is a serious pest that causes devastating losses to many major crops, including corn, rice, sugarcane, and peanut. Camptothecin (CPT) is a bioactive secondary metabolite of the woody plant Camptotheca acuminata, which has shown high toxicity to various pests. However, the effect of CPT against S. frugiperda remains unknown. Results In this study, bioassays have been conducted on the growth inhibition of CPT on S. frugiperda larvae. Histological and cytological changes were examined in the midgut of larvae fed on an artificial diet supplemented with 1.0 and 5.0 µg/g CPT. The potential molecular mechanism was explored by comparative transcriptomic analyses among midgut samples obtained from larvae under different treatments. A total of 915 and 3560 differentially expressed genes (DEGs) were identified from samples treated with 1.0 and 5.0 µg/g CPT, respectively. Among the identified genes were those encoding detoxification-related proteins and components of peritrophic membrane such as mucins and cuticle proteins. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses indicated that part of DEGs were involved in DNA replication, digestion, immunity, endocrine system, and metabolism. Conclusions Our results provide useful information on the molecular basis for the impact of CPT on S. frugiperda and for future studies on potential practical application.

show abstract

Role of EPG in Developing and Assessing Control Methods for Heteropteran Crop Pests

Panizzi

Lucini

Mitchell

2021

Entomology in Focus

View full text Add to dashboard Cite

Homozygosis of Bt locus increases Bt protein expression and the control of Spodoptera frugiperda (Lepidoptera: Noctuidae) in maize hybrids

Cited by 12 publications

References 43 publications

Transgene behavior in Zea mays L. crosses across different genetic backgrounds: Segregation patterns, cry1Ab transgene expression, insecticidal protein concentration and bioactivity against insect pests

Transgene behavior in Zea mays L. crosses across different genetic backgrounds: Segregation patterns, cry1Ab transgene expression, insecticidal protein concentration and bioactivity against insect pests

Growth inhibition of Spodoptera frugiperda larvae by camptothecin correlates with alteration of the structures and gene expression profiles of the midgut

Role of EPG in Developing and Assessing Control Methods for Heteropteran Crop Pests

Contact Info

Product

Resources

About