Homogeneous capture and heterogeneous separation of proteins by PEG-functionalized ionic liquid–water systems

Abstract

An efficient homogeneous capture and heterogeneous separation strategy for proteins is reported using PEG-functionalized ionic liquids with LCST phase behavior in water.

Methods for producing microstructured hydrogels for targeted applications in biology

“…The interactions between ionic groups play a key role in the phase separation and are strongly affected by the pH and the ionic strength of the solution [154]. Indeed, when sufficiently charged, proteins undergo repulsive interactions that preclude phase separation, even in the presence of PEG; this effect has been demonstrated for PEG solutions with lysozyme, papain, cytochrome c and IgG antibodies [68,155]. The type of ion also has an important effect.…”

“…Liquid-liquid phase separation (LLPS) is a one-step procedure where the microstructure (mainly limited to spherical and percolated structures) can be tuned by modifying physico-chemical parameters as temperature, concentration, or molecular weight [64]. While many of the main applications of this technique are largely focused on extraction and purification of material such as DNA [65,66], proteins [67][68][69], and metals [70], the opportunities are significant for producing microstructured hydrogel materials with biomedical applications such as drug delivery and tissue regeneration [71][72][73] or even to form synthetic membraneless organelles for modeling intracellular processes [74]. Limitations of LLPS include a loss of temporal control over the microstructure due to rapid phase separation [73,75], and biocompatible approaches to overcome this issue have been based mainly on interface stabilization by copolymers [76] and Pickering particles [77].…”

Temperature-Responsive Deep Eutectic Solvents for Highly Selective Separation of 5-Hydroxymethylfurfural from Reaction Mixture

“…Partition coefficient ( K ) and separation factor ( SF ) of HMF and fructose as well as the extraction efficiencies ( EE %) of HMF were calculated according to the following equations: K HMF = C DES , HMF / C water , HMF K Fructose = C DES , Fructose / C water , Fructose S F = K HMF / K Fructose E E HMF % = false[ ( C DES , HMF × V DES ) / ( C DES , HMF × V DES + C water , HMF × V water ) false] × 100 % where C DES,HMF , C DES,fructose ,...…”

“…Partition coefficient ( K ) and separation factor ( SF ) of HMF and fructose as well as the extraction efficiencies ( EE %) of HMF were calculated according to the following equations: where C DES,HMF , C DES,fructose , C water,HMF , and C water,fructose are the molar concentrations of HMF and fructose in the DES-rich phase and H 2 O-rich phase, V DES and V water are the volumes of the DES-rich phase and water-rich phase, and K HMF and K Fructose are the partition coefficient of HMF and fructose, respectively.…”