Homocysteinemia Correlates with Plasma Thiol Redox Status in Patients with End-Stage Renal Disease

Palleschi, Simonetta; Angelis, Sandro De; Rossi, Barbara; Diana, Loretta; Papa, Vincenza; Severini, Giancarlo; Splendiani, G

doi:10.1159/000113527

Cited by 7 publications

(6 citation statements)

References 61 publications

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“…These results indicate that the level of oxidative stress is higher in patients with ESRD than in healthy individuals. Our findings are consistent with previous reports wherein patients with ESRD have been shown to have decreased plasma protein thiols and increased S-thiolated proteins (9,13,14). In addition, Przemysław et al (14) found that the total concentration of glutathione, which is a low molecular weight thiol-tripeptide, was decreased in terminal renal failure patients and that total concentrations of the remaining thiols in these patients were significantly increased compared to those in the healthy subjects.…”

Section: Discussionsupporting

confidence: 93%

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Budak¹,

Kahvecioğlu²,

Çelik³

et al. 2017

Turk Neph Dial Transpl

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OBJECTIVE:We aimed to evaluate thiol/disulfide homeostasis as an oxidative stress parameter in end-stage renal disease patients receiving different substitutive therapies. MATERIAL and METHODS:Twenty-four renal transplantation (46.2±8.1 years), 24 peritoneal dialysis (47.8±8.5 years), and 25 hemodialysis patients (45.7±16.0 years), and 24 healthy controls (47.6±8.1 years) were included. Serum native thiol, total thiol, and disulfide levels were measured for all subjects. RESULTS:Serum total thiol and native thiol levels in the ESRD patients were significantly lower than those in the controls (P<0.001). Native and total thiol levels were significantly lower in PD (256 ± 45 μmol/L, and 287 ± 57 μmol/L) compared to HD (324 ± 44 μmol/L and 356 ± 46 μmol/L) group (p<0.01 and p=0.03). Disulfide/native thiol and disulfide/total thiol levels increased significantly in the PD and transplantation patients compared to control group (P<0.001). Positive correlation was observed between total thiols (r= 0.491 P<0.001), disulfide/native thiol ratio (r=0.383, P<0.001), and blood urea nitrogen; disulfide levels and blood urea nitrogen showed a negative correlation (-0.415, P<0.001). Disulfide levels and glomerular filtration rate showed a positive correlation (r=0.276, P=0.030). CONCLUSION:Serum thiol/disulfide homeostasis can be an indicator of oxidative stress in end-stage renal disease and that thiol/disulfide homeostasis analysis should be included in the routine monitoring of end-stage renal disease patients. KEy wORDS: Hemodialysis, Peritoneal dialysis, Renal transplantationÖz AMAÇ: Çalışmada son dönem böbrek yetmezliği hastalığı için farklı renal replasman tedavisi alan hastalarda bir oksidatif stres belirteci olarak tiol/disülfit dengesinin araştırılması amaçlanmıştır. GEREÇ ve yÖNTEMLER:Yirmi dört böbrek nakli yapılmış (46,2±8,1 yaş), 25 hemodiyaliz (45,7±16,0 yaş) ve 24 periton diyalizi (47,8±8,5 yaş) hastaları ile 24 sağlıklı kontrol (47,6±8,1 yaş) çalışmaya dahil edildi. Tüm olgularda nativ tiyol, total tiyol ve disülfit seviyeleri ölçüldü. BULGULAR:Hastalarda kontrol grubu ile karşılaştırıldığında total tiyol ve nativ tiyol seviyeleri anlamlı olarak düşüktü (p<0,001). Nativ ve total tiyol düzeyi periton diyalizi hastalarında (256±45 μmol/L ve 287±57 μmol/L) hemodiyaliz hastalarına (324±44 μmol/L ve 356±46 μmol/L) göre anlamlı düşüktü (p<0,01 ve p=0,03). Periton diyalizi ve transplantasyon hastalarında disulfit/nativ tiyol ve disülfit/total tiyol düzeyleri kontrol grubuna gore anlamlı olarak yüksek bulundu (p<0,001). Total tiyol (r=0,491 P<0,001) ve disulfit/nativ tiyol oranı (r=0,383, P<0,001) ile kan üre nitrojeni arasında pozitif korelasyon; disülfit seviyeleri ve kan üre nitrojeni arasında negatif korelasyon (-0,415, P<0,001) saptandı. Disülfit seviyeleri ve glomerülar filtrasyon değeri arasında pozitif korelasyon (r=0,276, P=0,030) bulundu. SONUÇ:Tedavi alan son dönem böbrek hastalarında rutin hasta takibinde serum tiyol/disülfit oranı oksidatif stres belirteci olarak kullanılabilir.

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Section: Discussionsupporting

confidence: 93%

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Budak¹,

Kahvecioğlu²,

Çelik³

et al. 2017

Turk Neph Dial Transpl

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“…The average total concentrations of Hcy, Cys, GSH and CysGly in healthy controls were within the published normal reference range (Hcy reference 3–12 μM, Cys reference 100 – 400 μM, GSH reference 2 – 10 μM and CysGly reference 30 – 60 μM) [35–39]. In thalassemia patients, however, the average concentrations of Hcy, Cys, GSH and CysGly were significantly elevated relative to controls.…”

Section: Resultsmentioning

confidence: 51%

Clinical assay of four thiol amino acid redox couples by LC–MS/MS: Utility in thalassemia

Suh¹,

Kim²,

Yavuz³

et al. 2009

Journal of Chromatography B

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The total concentrations of four sulfur amino acid (SAA) metabolite redox couples (reduced and oxidized forms of homocysteine, cysteine, glutathione, and cysteinylglycine) in human blood are assayed with a simple and sensitive method by liquid chromatography-electrospray positive ionization tandem mass spectrometry. To prevent ex vivo thiol oxidation, iodoacetamide (IAM) is used immediately following the blood draw. To selectively enrich for S-carboxyamidomethylated SAA, and other cationic amino acids metabolites, proprietary strong cation-exchange solid phase extraction tips are used. Analytes are further derivatized with isopropylchloroformate (IPCF) to esterify the amino and the carboxylic groups. Double derivatization with IAM and IPCF improves the reverse phase liquid chromatography separation of SAA metabolites. The use of detection mode of multiple-reaction monitoring (MRM) allows sensitive and specific simultaneous detection of SAA. The internal standards used to account for the matrix effects of human plasma and erythrocytes were plant glutathione analogue, homoglutathione, and stable isotopes of cystine and homocystine. The method was validated for its linearity, accuracy, and precision. Excellent linearity of detection (r2 > 0.98) was observed over relevant ranges for plasma and erythrocyte samples, and the limits of detection was established to be between 5 and 20 nM. Relative standard deviations were <9% for within-day variations and <15% for between day variations. The method was used to assess thiol redox states in plasma and erythrocytes isolated from healthy subjects and thalassemia patients.

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“…Similar results were also obtained when plasma P-SH were expressed as nanomoles per milligram of protein, calculated using total plasma protein concentrations [ 112 ]. This result was also confirmed when considering possible dialysis-induced changes in the plasma protein concentration [ 113 ], which can dramatically affect the measured values of total plasma thiols [ 105 ]. Moreover, the total plasma thiol levels decreased significantly even after haemodiafiltration [ 113 ].…”

Section: P-sh and S -Thiolated Proteins In Hd Pati...mentioning

confidence: 60%

“…Several studies have measured the levels of P-SH and S -thiolated proteins in HD patients before (pre-HD) and after an HD session (post-HD) ( Table 3 ). Some authors reported that the levels of total plasma thiols (P-SH + LMM-SH) significantly decreased post-HD, returning to the range measured in healthy subjects [ 111 , 112 , 113 ]. The plasma P-SH levels in ESRD patients on HD were significantly lower than those in healthy subjects.…”

Section: P-sh and S -Thiolated Proteins In Hd Pati...mentioning

confidence: 98%

Blood Thiol Redox State in Chronic Kidney Disease

Garavaglia

Giustarini

Colombo

et al. 2022

IJMS

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Thiols (sulfhydryl groups) are effective antioxidants that can preserve the correct structure of proteins, and can protect cells and tissues from damage induced by oxidative stress. Abnormal levels of thiols have been measured in the blood of patients with moderate-to-severe chronic kidney disease (CKD) compared to healthy subjects, as well as in end-stage renal disease (ESRD) patients on haemodialysis or peritoneal dialysis. The levels of protein thiols (a measure of the endogenous antioxidant capacity inversely related to protein oxidation) and S-thiolated proteins (mixed disulphides of protein thiols and low molecular mass thiols), and the protein thiolation index (the molar ratio of the S-thiolated proteins to free protein thiols in plasma) have been investigated in the plasma or red blood cells of CKD and ESRD patients as possible biomarkers of oxidative stress. This type of minimally invasive analysis provides valuable information on the redox status of the less-easily accessible tissues and organs, and of the whole organism. This review provides an overview of reversible modifications in protein thiols in the setting of CKD and renal replacement therapy. The evidence suggests that protein thiols, S-thiolated proteins, and the protein thiolation index are promising biomarkers of reversible oxidative stress that could be included in the routine monitoring of CKD and ESRD patients.

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Homocysteinemia Correlates with Plasma Thiol Redox Status in Patients with End-Stage Renal Disease

Cited by 7 publications

References 61 publications

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Serum Thiol/Disulfide Homeostasis in Hemodialysis, Peritoneal Dialysis, and Renal Transplantation Patients

Clinical assay of four thiol amino acid redox couples by LC–MS/MS: Utility in thalassemia

Blood Thiol Redox State in Chronic Kidney Disease

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