Homocysteine increases monocyte and T-cell adhesion to human aortic endothelial cells

Koga, Takuro; Claycombe, Kate J.; Meydani, Mohsen

doi:10.1016/s0021-9150(01)00670-0

Cited by 88 publications

(61 citation statements)

References 53 publications

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“…13) Briefly, HUVECs (1ϫ10 5 /ml), were distributed into 96-well plates and were allowed to reach confluence, then the growth medium was supplemented with FA at the indicated concentrations for 18 h, and cells were exposed to the 60 Co source (10 Gy). 1ϫ 10 6 /ml U937 cells were added to each HUVEC-containing well and incubation continued for 1 h. Non-adherent cells were removed by two gentle washes with PBS, and the number of bound U937 cells was determined by MTT assay.…”

Section: Methodsmentioning

confidence: 99%

Ferulic Acid Attenuates Adhesion Molecule Expression in Gamma-Radiated Human Umbilical Vascular Endothelial Cells

Qian

Wang

et al. 2010

Biological & Pharmaceutical Bulletin

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Radiation induces an important inflammatory response in the irradiated organs, characterized by leukocyte infiltration and vascular changes. Since adhesion molecules play an important role in facilitating the immune response at the inflammation sites, interfering with the expression of these molecules may be an important therapeutic target of radiation induced inflammation. Many adhesion molecules such as intercellular cell adhesion molecule 1 (ICAM-1), and vascular cell adhesion molecule 1 (VCAM-1) have been identified in radiation. Ferulic acid (FA), an effective radioprotector during radiotherapy, is widely used in endothelium protection. The present study examined the effect of FA on the induction of adhesion molecules by gamma-radiation and the mechanisms of its effect in gamma-irradiated human umbilical vein endothelial cells (HUVECs). HUVECs were pretreated for 18 h with FA and then exposed to 10 Gy radiation. The result of cell adhesion assay showed FA inhibited radiation-induced U937 adhesion to HUVECs. FA prevented induction of ICAM-1 and VCAM-1 expression in a concentration-dependent manner after stimulation with radiation at the level of mRNA and protein. Inhibitors of the extracellular signal regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) pathways were used to determine which pathway was involved in FA action; the result showed that the inhibitory effect of FA on adhesion molecule expression was mediated by the blockade of JNK. FA appears to be a potential therapeutic agent for treating various inflammatory disorders including radiation induced inflammation.

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Section: Methodsmentioning

confidence: 99%

Ferulic Acid Attenuates Adhesion Molecule Expression in Gamma-Radiated Human Umbilical Vascular Endothelial Cells

Qian

Wang

et al. 2010

Biological & Pharmaceutical Bulletin

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“…U937 cells were labeled with the fluorescent dye BCECF-AM (Molecular Probes) as described 14 and added at an effector to target (E/T) ratio of 10:1 to HCAECs treated or not with 10 g/mL of sCD40L for 24 hours. After 1 hour incubation, HCAECs were washed and attached cells lysed in 50 mmol Tris buffer with 0.1% SDS.…”

Section: Adhesion Assaymentioning

confidence: 99%

A20 Protects From CD40-CD40 Ligand-Mediated Endothelial Cell Activation and Apoptosis

et al. 2003

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Background-CD40/CD40 ligand (CD40L) signaling is a potent activator of endothelial cells (ECs) and promoter of atherosclerosis. In this study, we investigate whether A20 (a gene we have shown to be antiinflammatory and antiapoptotic in ECs) can protect from CD40/CD40L-mediated EC activation. Methods and Results-Overexpression of CD40, in a transient transfection system, activates the transcription factor NF-B and upregulates IB␣, E-selectin, and tissue factor (TF) reporter activity. Coexpression of A20 inhibits NF-B and upregulation of IB␣ and E-Selectin but not TF, suggesting that CD40 induces TF in a non-NF-B-dependent manner. In human coronary artery ECs (HCAECs), adenovirus-mediated overexpression of A20 blocks physiological, CD40-induced activation of NF-B, upstream of IB␣ degradation (Western blot) and subsequently upregulation of ICAM-1, VCAM-1, and E-selectin (flow cytometry). Although A20 does not block TF transcription its expression in HCAECs inhibits TF induction (colorimetric assay and RT-PCR) by blunting CD40 upregulation. We demonstrate that CD40 signaling induces apoptosis in a proinflammatory microenvironment. A20 overexpression protects from CD40-mediated EC apoptosis (DNA content analysis and trypan blue exclusion). We also demonstrate that signaling through CD40L activates NF-B and induces apoptosis in ECs, both of which are inhibited by A20 overexpression. Conclusion-A20 works at multiple levels to protect ECs from CD40/CD40L mediated activation and apoptosis.A20-based therapy could be beneficial for the treatment of vascular diseases such as atherosclerosis and transplantassociated vasculopathy.

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“…Wu et al (2012a) [30] reported that methionine deficiency can decrease serum IgG, IgA and IgM content significantly, which suggests that the humoral immune function is injured. In addition, the level of the methionine is higher in the immune response than normal condition [48] [49] [50], which suggesting that it has close relationship between the methionine and immune response or disease resistance. Increase the dose of methionine in the diet of chicken which infected by Newcastle disease virus, the level of IgG increased too [51].…”

Section: Effects Of Methionine On Humoral Immunitymentioning

confidence: 99%

Effects of Methionine on the Immune Function in Animals

Ruan¹,

Peng²,

Wu³

2017

Health

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Nutrition and immunity are the hot topics in animal's production, and the effects of methionine on the immunity are already confirmed as the deep research on the nutrition of amino acid and immune function. However, the relationship of the methionine and immunity has not been elucidated clearly, this review aims to clarify the effects of methionine on immune function in the aspects of growth and development immune organs, the histological structure of the immune organs, non-specific immunity, humoral immunity, cellular immunity and cytokines, and to provide foundations for further studies on the relationship between methionine and immune function.

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Homocysteine increases monocyte and T-cell adhesion to human aortic endothelial cells

Cited by 88 publications

References 53 publications

Ferulic Acid Attenuates Adhesion Molecule Expression in Gamma-Radiated Human Umbilical Vascular Endothelial Cells

Ferulic Acid Attenuates Adhesion Molecule Expression in Gamma-Radiated Human Umbilical Vascular Endothelial Cells

A20 Protects From CD40-CD40 Ligand-Mediated Endothelial Cell Activation and Apoptosis

Effects of Methionine on the Immune Function in Animals

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