Homocitrate Synthase Expression and Lysine Content in Fruiting Body of Different Developmental Stages in Flammulina velutipes

Liu, Fang; Wang, Wei; Chen, Bingzhi; Xie, Baogui

doi:10.1007/s00284-015-0791-0

Cited by 14 publications

(31 citation statements)

References 30 publications

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“…New HD and PR loci of F. velutipes were identified using the draft genomes of two mating-compatible strains; F. velutipes strain L11 and F. velutipes strain W23 (Liu et al 2015; Wang et al 2015b; Zeng et al 2015). The draft genome of strain L11 consisted of 1858 scaffolds covering 34.75 Mb and 11,526 predicted genes.…”

Section: Resultsmentioning

confidence: 99%

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Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

Wang

Lian

et al. 2016

G3 Genes|Genomes|Genetics

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The initiation of sexual development in the important edible and medicinal mushroom Flammulina velutipes is controlled by special genes at two different, independent, mating type (MAT) loci: HD and PR. We expanded our understanding of the F. velutipes mating type system by analyzing the MAT loci from a series of strains. The HD locus of F. velutipes houses homeodomain genes (Hd genes) on two separated locations: sublocus HD-a and HD-b. The HD-b subloci contained strain-specific Hd1/Hd2 gene pairs, and crosses between strains with different HD-b subloci indicated a role in mating. The function of the HD-a sublocus remained undecided. Many, but not all strains contained the same conserved Hd2 gene at the HD-a sublocus. The HD locus usually segregated as a whole, though we did detect one new HD locus with a HD-a sublocus from one parental strain, and a HD-b sublocus from the other. The PR locus of F. velutipes contained pheromone receptor (STE3) and pheromone precursor (Pp) genes at two locations, sublocus PR-a and PR-b. PR-a and PR-b both contained sets of strain-specific STE3 and Pp genes, indicating a role in mating. PR-a and PR-b cosegregated in our experiments. However, the identification of additional strains with identical PR-a, yet different PR-b subloci, demonstrated that PR subloci can recombine within the PR locus. In conclusion, at least three of the four MAT subloci seem to participate in mating, and new HD and PR loci can be generated through intralocus recombination in F. velutipes.

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Section: Resultsmentioning

confidence: 99%

“…Kong of the Rural Development Administration (RDA) in Korea. Genome sequences of F. velutipes strain L11 and strain W23 were obtained from our lab (Zeng et al 2015; Liu et al 2015; Wang et al 2015b). Genomic DNA was extracted from mycelium using a CTAB method (Stajich et al 2010).…”

Section: Methodsmentioning

confidence: 99%

Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

Wang

Lian

et al. 2016

G3 Genes|Genomes|Genetics

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“…Monokaryon strains W23 and dikaryon strain H1123 (a derivative of hybridization of monokaryon strains W23 and L11), provided by the Fujian Edible Fungi Germplasm Resource Collection Center of China [39]. For genomic DNA extraction using a modified CTAB (cetyltrimethyl ammonium bromide) approach [40], strain W23 was grown in liquid potato dextrose broth (PDB) at 25 °C for 10 days, and the mycelia were stored at −80 °C after being harvested.…”

Section: Methodsmentioning

confidence: 99%

“…APHZ00000000; BioProject: 191864). For analysis of gene structure, all of the transcriptome reads at each developmental stage [39,41] were mapped to the full-length Fv-JRL1 gene, as well as 2 kbp each of upstream and downstream sequences, using Zoom lite with a maximum of 40 mismatched base pairs [39,42]. In addition, comparison of the sequences of Fv-JRL1 in W23 and L11 (GenBank Accession No.…”

Section: Methodsmentioning

confidence: 99%

“…Twenty-microliter reaction mixtures were prepared in accordance with manufacturer’s instructions using Bio-Rad iTaq TM Universal SYBR Green Supermix (Bio-Rad). The qRT-PCR protocols were as follows: initial denaturation at 94 °C for 30 s; 40 cycles of 5 s at 95 °C, 30 s at 58 °C, and 65 °C for 5 s. Primer Premier 5.0 was applied for design of primers for Fv-JRL1 and reference gene glyceraldehyde-3-phosphate dehydrogenase ( gapdh ) (Table S1) [39,43,44]. The transcript levels of target genes were calculated using 2 −ΔΔ C t method [45].…”

Section: Methodsmentioning

confidence: 99%

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A Jacalin-Related Lectin Regulated the Formation of Aerial Mycelium and Fruiting Body in Flammulina velutipes

Chen

Long

et al. 2016

IJMS

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Flammulina velutipes, one of the most popular mushroom species in the world, has been recognized as a useful model system to study the biochemical and physiological aspects of the formation and elongation of fruit body. However, few reports have been published on the regulation of fruiting body formation in F. velutipes at the molecular level. In this study, a jacalin-related lectin gene from F. velutipes was characterized. The phylogenetic tree revealed that Fv-JRL1 clustered with other basidiomycete jacalin-like lectins. Moreover, the transcriptional pattern of the Fv-JRL1 gene in different developmental stages of F. velutipes implied that Fv-JRL1 could be important for formation of fruit body. Additionally, RNA interference (RNAi) and overexpression analyses provided powerful evidence that the lectin gene Fv-JRL1 from F. velutipes plays important roles in fruiting body formation.

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Overexpression of the saccharopine dehydrogenase gene improves lysine biosynthesis in Flammulina velutipes

Liu

Jiang

et al. 2019

J Basic Microbiol

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Saccharopine dehydrogenase (EC 1.5.1.7) regulates the last step of fungal lysine biosynthesis. The gene (Fvsdh) encoding saccharopine dehydrogenase was identified and cloned from the whole genome of Flammulina velutipes. The genomic DNA of Fvsdh is 1257 bp, comprising three introns and four exons. The full-length complementary DNA of Fvsdh comprises 1107 bp with a deduced amino acid sequence of 368 residues. A 1,000-bp promoter sequence containing the TATA box, CAAT box, and several putative cis-acting elements was also identified. The results of tissue expression analysis showed that the expression level of the Fvsdh gene was higher in the pileus than in the stipe whether in the elongation or maturation stage. Further research showed that the lysine contents were 3.03 and 2.95 mg/g in maturation-pileus and elongation-pileus, respectively. In contrast, the lysine contents were 2.49 and 2.07 mg/g in elongation-stipe and maturation-stipe, respectively. To study the function of Fvsdh, we overexpressed Fvsdh in F. velutipes and found that Fvsdh gene expression was increased from 1.1-to 3-fold in randomly selected transgenic strains. The lysine contents were also increased from 1.12-to 1.3-fold in these five transformants, except for strain T3, in which the lysine contents were the same as the control. These results indicate that the expression of the Fvsdh gene can affect the lysine content of F. velutipes. K E Y W O R D SFlammulina velutipes, lysine content, saccharopine dehydrogenase

show abstract

Homocitrate Synthase Expression and Lysine Content in Fruiting Body of Different Developmental Stages in Flammulina velutipes

Cited by 14 publications

References 30 publications

Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

Advances in Understanding Mating Type Gene Organization in the Mushroom-Forming FungusFlammulina velutipes

A Jacalin-Related Lectin Regulated the Formation of Aerial Mycelium and Fruiting Body in Flammulina velutipes

Overexpression of the saccharopine dehydrogenase gene improves lysine biosynthesis in Flammulina velutipes

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