Homing of Adipose-Derived Stem Cells to a Tendon-Derived Hydrogel: A Potential Mechanism for Improved Tendon-Bone Interface and Tendon Healing

Franklin, Austin; Min, Jung Gi; Oda, Hiroki; Kaizawa, Yukitoshi; Leyden, Jacinta; Wang, Zhen; Fox, Paige M.

doi:10.1016/j.jhsa.2020.05.003

Cited by 13 publications

(9 citation statements)

References 36 publications

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“…Franklin et al in 2020 demonstrated the homing of systemic ADSC in proper human decellularized tendon derived from hydrogel scaffold, in rats’ Achilles tendon defects. Flow cytometric analysis revealed a higher homing of ADSC in the injury site in the treated group [ 67 ].…”

Section: Resultsmentioning

confidence: 99%

“…No side effects or signs of rejection have been reported in all of the studies examined. Concerning biomaterials and innovative scaffolds are examined in vivo as stable supports for ADSC/SVF, especially in high tendon tissue defects [ 64 , 65 , 66 , 67 , 68 ]. Some of the newest scaffolds include N-PLGA, PGA/PLA and tHG decellularized tendon scaffold.…”

Section: Discussionmentioning

confidence: 99%

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Efficacy of Adipose-Derived Mesenchymal Stem Cells and Stromal Vascular Fraction Alone and Combined to Biomaterials in Tendinopathy or Tendon Injury: Systematic Review of Current Concepts

et al. 2023

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Background and Objectives: Tendon injury and tendinopathy are among the most frequent musculoskeletal diseases and represent a challenging issue for surgeons as well as a great socio-economic global burden. Despite the current treatments available, either surgical or conservative, the tendon healing process is often suboptimal and impaired. This is due to the inherent scarce ability of tendon tissue to repair and return itself to the original structure. Recently, Adipose-derived mesenchymal stem cells (ADSC) and stromal vascular fraction (SVF) have gained a central interest in the scientific community, demonstrating their effectiveness in treatments of acute and chronic tendon disorders in animals and humans. Either enzymatic or mechanical procedures to obtain ADSC and SVF have been described and used in current clinical practice. However, no unified protocols and processes have been established. Materials and Methods: This systematic review aims at providing a comprehensive update of the literature on the clinical application of ADSC enzymatically or mechanically processed to obtain SVF, alone and in association with biomaterials in the local treatment of tendinopathy and tendon injury in vivo, in animal models and humans. The study was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Results: Thirty-two articles met our inclusion criteria, with a total of 18 studies in animals, 10 studies in humans and 4 studies concerning the application of biomaterials in vivo in animals. The review of the literature suggests that ADSC/SVF therapy can represent a promising alternative in tendonregenerative medicine for the enhancement of tendon healing. Conclusions: Nevertheless, further investigations and randomized control trials are needed to improve the knowledge, standardize the procedures and extend the consensus on their use for such applications.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Efficacy of Adipose-Derived Mesenchymal Stem Cells and Stromal Vascular Fraction Alone and Combined to Biomaterials in Tendinopathy or Tendon Injury: Systematic Review of Current Concepts

et al. 2023

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“…On the other hand, due to the difficulties in the functional assessment of patients after ACL reconstruction, most studies are limited to animal models. The role of biomaterials in promoting tendon-bone healing is mainly to act as scaffolds of fibrocytes and osteoblasts in bone tunnels to promote bone formation ( Bi et al, 2021 ; Lv et al, 2022 ; Han et al, 2023 ), and secondly to act as drug carriers to fix drugs in the knee joint for continuous and effective release within a certain period of time ( Lee et al, 2017 ; Franklin et al, 2020 ; Huang et al, 2020 ), so as to achieve the optimal purpose of promoting tendon-bone healing. Therefore, the application of biomaterials should be widely combined with other therapeutic strategies, such as stem cells, cytokines, etc.…”

Section: Discussion and Outlookmentioning

confidence: 99%

Strategies to promote tendon-bone healing after anterior cruciate ligament reconstruction: Present and future

Tian

Zhang

Kang

2023

Front. Bioeng. Biotechnol.

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At present, anterior cruciate ligament (ACL) reconstruction still has a high failure rate. Tendon graft and bone tunnel surface angiogenesis and bony ingrowth are the main physiological processes of tendon-bone healing, and also the main reasons for the postoperative efficacy of ACL reconstruction. Poor tendon-bone healing has been also identified as one of the main causes of unsatisfactory treatment outcomes. The physiological process of tendon-bone healing is complicated because the tendon-bone junction requires the organic fusion of the tendon graft with the bone tissue. The failure of the operation is often caused by tendon dislocation or scar healing. Therefore, it is important to study the possible risk factors for tendon-bone healing and strategies to promote it. This review comprehensively analyzed the risk factors contributing to tendon-bone healing failure after ACL reconstruction. Additionally, we discuss the current strategies used to promote tendon-bone healing following ACL reconstruction.

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“…However, the presence of chemotactic growth factor, such as platelet-derived growth factor, vascular endothelial growth factor, and FB growth factor, likely enhance the migration of not only the exogenous cells, but also promote the migration of endogenous stem cells to the wound in vivo. 20 – 23 We have used a rat model to examine the homing of systemic stem cells to cHG and cHG +ADSCs, noting an increase in the attraction of endogenous cells to seeded cHG. 24 …”

Section: Discussionmentioning

confidence: 99%

Analysis of Cell-seeded, Collagen-rich Hydrogel for Wound Healing

Leon

Williams

Wang

et al. 2020

Plastic and Reconstructive Surgery - Global Open

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Background: Our laboratory has previously developed a novel collagen-rich hydrogel (cHG), which significantly increases the speed of wound healing in diabetic rats. Methods: In this study, we examine the in vitro survival and migration of fibroblasts, endothelial cells, and adipose-derived stem cells in a novel cHG. Furthermore, we test the ability of adipose-derived stem cell–seeded cHG to support cell survival and accelerate healing in vivo. Results: In vitro, cell survival within the cHG was retained for 25 days. We were unable to detect cellular migration into, out of, or through cHG. In the in vivo model, bioluminescence of stem cells seeded within the cHG in diabetic rat wounds was detected until day 10. Rate of wound closure was higher for cHG plus adipose-derived stem cells versus control from day 2 until day 16 and significant on days 6, 8, and 12 (P < 0.05). This significant difference was also observed on day 16 by histology (P ≤ 0.05). Conclusions: We conclude that cHG is a good candidate for delivering adipose-derived stem cells, endothelial cells, and fibroblasts to wounds. Future studies will determine whether the delivery of combinations of different cell lines in cHG further enhances wound healing.

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Homing of Adipose-Derived Stem Cells to a Tendon-Derived Hydrogel: A Potential Mechanism for Improved Tendon-Bone Interface and Tendon Healing

Cited by 13 publications

References 36 publications

Efficacy of Adipose-Derived Mesenchymal Stem Cells and Stromal Vascular Fraction Alone and Combined to Biomaterials in Tendinopathy or Tendon Injury: Systematic Review of Current Concepts

Efficacy of Adipose-Derived Mesenchymal Stem Cells and Stromal Vascular Fraction Alone and Combined to Biomaterials in Tendinopathy or Tendon Injury: Systematic Review of Current Concepts

Strategies to promote tendon-bone healing after anterior cruciate ligament reconstruction: Present and future

Analysis of Cell-seeded, Collagen-rich Hydrogel for Wound Healing

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