Analysis of Cell-seeded, Collagen-rich Hydrogel for Wound Healing

Leon, Daniel Sotelo; Williams, Tokoya; Wang, Zhen; Leyden, Jacinta; Franklin, Austin; Kaizawa, Yukitoshi; Fox, Paige M.

doi:10.1097/gox.0000000000003049

Cited by 3 publications

(1 citation statement)

References 26 publications

(29 reference statements)

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“…To attain similar customizability to synthetics in organic hydrogels, various engineered features, such as embedded pharmaceutical carriers, pharmaceutical-scaffold covalent bonding, and composite hydrogels, have been designed [42,47,48]. The stem cell homing [49], wound healing [50], and antibiotic delivery characteristics of cHG make it an ideal hydrogel for further enhancement to tackle biofilms and other infected wounds [21,51].…”

Section: Plos Onementioning

confidence: 99%

Human-Derived collagen hydrogel as an antibiotic vehicle for topical treatment of bacterial biofilms

Jarman,

Burgess,

Sharma

et al. 2024

PLoS ONE

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The complexity of chronic wounds creates difficulty in effective treatments, leading to prolonged care and significant morbidity. Additionally, these wounds are incredibly prone to bacterial biofilm development, further complicating treatment. The current standard treatment of colonized superficial wounds, debridement with intermittent systemic antibiotics, can lead to systemic side-effects and often fails to directly target the bacterial biofilm. Furthermore, standard of care dressings do not directly provide adequate antimicrobial properties. This study aims to assess the capacity of human-derived collagen hydrogel to provide sustained antibiotic release to disrupt bacterial biofilms and decrease bacterial load while maintaining host cell viability and scaffold integrity. Human collagen harvested from flexor tendons underwent processing to yield a gellable liquid, and subsequently was combined with varying concentrations of gentamicin (50–500 mg/L) or clindamycin (10–100 mg/L). The elution kinetics of antibiotics from the hydrogel were analyzed using liquid chromatography-mass spectrometry. The gel was used to topically treat Methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium perfringens in established Kirby-Bauer and Crystal Violet models to assess the efficacy of bacterial inhibition. 2D mammalian cell monolayers were topically treated, and cell death was quantified to assess cytotoxicity. Bacteria-enhanced in vitro scratch assays were treated with antibiotic-embedded hydrogel and imaged over time to assess cell death and mobility. Collagen hydrogel embedded with antibiotics (cHG+abx) demonstrated sustained antibiotic release for up to 48 hours with successful inhibition of both MRSA and C. perfringens biofilms, while remaining bioactive up to 72 hours. Administration of cHG+abx with antibiotic concentrations up to 100X minimum inhibitory concentration was found to be non-toxic and facilitated mammalian cell migration in an in vitro scratch model. Collagen hydrogel is a promising pharmaceutical delivery vehicle that allows for safe, precise bacterial targeting for effective bacterial inhibition in a pro-regenerative scaffold.

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Section: Plos Onementioning

confidence: 99%

Human-Derived collagen hydrogel as an antibiotic vehicle for topical treatment of bacterial biofilms

Jarman,

Burgess,

Sharma

et al. 2024

PLoS ONE

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Regenerative Potential of Adipose Derived Stem Cells in Wound Healing and Scar Management: A Review

Milan,

Takla,

Hayek

et al. 2024

Curr Derm Rep

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Improving Cutaneous Wound Healing in Diabetic Mice Using Naturally Derived Tissue‐Engineered Biological Dressings Produced under Serum‐Free Conditions

Safoine,

Paquette,

Gingras

et al. 2024

Stem Cells International

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Long-term diabetes often leads to chronic wounds refractory to treatment. Cell-based therapies are actively investigated to enhance cutaneous healing. Various cell types are available to produce biological dressings, such as adipose-derived stem/stromal cells (ASCs), an attractive cell source considering their abundancy, accessibility, and therapeutic secretome. In this study, we produced human ASC-based dressings under a serum-free culture system using the self-assembly approach of tissue engineering. The dressings were applied every 4 days to full-thickness 8-mm splinted skin wounds created on the back of polygenic diabetic NONcNZO10/LtJ mice and streptozotocin-induced diabetic K14-H2B-GFP mice. Global wound closure kinetics evaluated macroscopically showed accelerated wound closure in both murine models, especially for NONcNZO10/LtJ; the treated group reaching 98.7% ± 2.3% global closure compared to 76.4% ± 11.8% for the untreated group on day 20 (p=0.0002). Histological analyses revealed that treated wounds exhibited healed skin of better quality with a well-differentiated epidermis and a more organized, homogeneous, and 1.6-fold thicker granulation tissue. Neovascularization, assessed by CD31 labeling, was 2.5-fold higher for the NONcNZO10/LtJ treated wounds. We thus describe the beneficial impact on wound healing of biologically active ASC-based dressings produced under an entirely serum-free production system facilitating clinical translation.

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Analysis of Cell-seeded, Collagen-rich Hydrogel for Wound Healing

Cited by 3 publications

References 26 publications

Human-Derived collagen hydrogel as an antibiotic vehicle for topical treatment of bacterial biofilms

Human-Derived collagen hydrogel as an antibiotic vehicle for topical treatment of bacterial biofilms

Regenerative Potential of Adipose Derived Stem Cells in Wound Healing and Scar Management: A Review

Improving Cutaneous Wound Healing in Diabetic Mice Using Naturally Derived Tissue‐Engineered Biological Dressings Produced under Serum‐Free Conditions

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