Holography in Otology

Bally, Gert von

doi:10.1007/978-3-540-38961-3_26

Cited by 10 publications

(6 citation statements)

References 10 publications

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“…Inside linear imaging modality methods, digital holographic microscopy (DHM) has becoming a powerful and versatile tool in many significant fields of Biophotonics, Life Sciences and Medicine [5][6][7]. DHM combines into a single platform high-quality imaging provided by microscopy, whole-object wavefront recovery provided by holography, and numerical processing capabilities provided by computers [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Single-shot, dual-mode, water-immersion microscopy platform for biological applications

et al. 2017

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A single-shot water-immersion digital holographic microscope combined with broadband (white light) illumination mode is presented. This double imaging platform allows conventional incoherent visualization with phase holographic imaging of inspected samples. The holographic architecture is implemented at the image space (that is, after passing the microscope lens), thus reducing the sensitivity of the system to vibrations and/or thermal changes in comparison to regular interferometers. Because of the off-axis holographic recording principle, quantitative phase images of live biosamples can be recorded in a single camera snapshot at full-field geometry without any moving parts. And, the use of water-immersion imaging lenses maximizes the achievable resolution limit. This dual-mode microscope platform is first calibrated using microbeads, then applied to the characterization of fixed cells (neuroblastoma, breast cancer, and hippocampal neuronal cells) and, finally, validated for visualization of dynamic living cells (hippocampal neurons).

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Section: Introductionmentioning

confidence: 99%

Single-shot, dual-mode, water-immersion microscopy platform for biological applications

et al. 2017

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“…[7][8][9] Due to its versatility, DHM has been successfully applied to real-time quantitative phase contrast imaging, 7 polarization microscopy imaging, 10 aberration lens compensation, 11 particle tracking, 12 3-D dynamic analysis of cells, 13 and in so many other disciplines in the fields of biophotonics, life sciences, and medicine. [14][15][16][17] DHM layouts typically use laser sources for generating interference fringes in the experimental configuration. However, coherent light is very sensitive to both the microstructure of the sample and any defect or dust in optical paths.…”

Section: Introductionmentioning

confidence: 99%

Spatially multiplexed interferometric microscopy with partially coherent illumination

et al. 2016

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We have recently reported on a simple, low cost, and highly stable way to convert a standard microscope into a holographic one [Opt. Express 22, 14929 (2014)]. The method, named spatially multiplexed interferometric microscopy (SMIM), proposes an off-axis holographic architecture implemented onto a regular (nonholographic) microscope with minimum modifications: the use of coherent illumination and a properly placed and selected one-dimensional diffraction grating. In this contribution, we report on the implementation of partially (temporally reduced) coherent illumination in SMIM as a way to improve quantitative phase imaging. The use of low coherence sources forces the application of phase shifting algorithm instead of off-axis holographic recording to recover the sample’s phase information but improves phase reconstruction due to coherence noise reduction. In addition, a less restrictive field of view limitation (1/2) is implemented in comparison with our previously reported scheme (1/3). The proposed modification is experimentally validated in a regular Olympus BX-60 upright microscope considering a wide range of samples (resolution test, microbeads, swine sperm cells, red blood cells, and prostate cancer cells).

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“…With a measurement accuracy in sub-micrometer range this methods have already been applied in many fields of industry [1] as well as for biomedical applications [2]. Furthermore, by utilization of modern CCD sensor technology and fast digital image processing an on-line application of these methods even in vivo [3] is possible.…”

Section: Introductionmentioning

confidence: 99%

Holographic interferometric microscopy systems for the application on biological samples

Kemper

Carl

Knoche

et al. 2004

Optical Metrology in Production Engineering

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Holographic interferometric metrology allows a fast, non destructive and quantitative high resolution full field detection of optical path length changes. Furthermore, by utilization of modern CCD sensor technology and digital image processing algorithms an on-line application of these methods even on biological specimens is possible. In combination with a microscopic resolution this offers new possibilities for the detection of variations of shape, micro movements or refractive index changes e. g. for the marker free analysis of cellular samples. Three holographic interferometric systems for microscopy applications based on digital holography, (speckle) interferometry and photorefractive crystals as holographic recording medium are introduced. Results of investigations on test charts and biological samples to characterize and optimize the lateral resolution as well as the resolution of the detected phase difference changes are presented and discussed. Finally, the applicability of the developed measurement techniques on living cells is demonstrated. INTRODUCTIONHolographic and speckle interferometry are well-established and frequently applied tools for non destructive testing (NDT) and the analysis of displacements, movements and vibrations. With a measurement accuracy in sub-micrometer range this methods have already been applied in many fields of industry [1] as well as for biomedical applications [2]. Furthermore, by utilization of modern CCD sensor technology and fast digital image processing an on-line application of these methods even in vivo [3] is possible. Holographic interferometric metrology allows a fast, non-contact and quantitative high resolution full field detection of optical path length changes. This offers in combination with a microscopic resolution new possibilities for the analysis of cellular tissues and cells. In the upcoming fields of life sciences and biophotonics there is a requirement for an optical instrumentation of timely and spatially high resolution analysis, measurement and documentation in microscopic dimensions. Here, applications are of particular interest to get enhanced information about cellular tissues and single cells e. g. the detection of shape variations and refractive index changes as well as the full field analysis of parameters like micro movements or the local distribution of elasticity. Up to the present, such parameters are in general not considered in cellular and sub cellular dimensions although they are already routinely used e. g. for defect detection and tumor diagnostics in the macroscopic scale. In that view, holographic interferometric microscopy tools open up new facilities for a marker free monitoring of life processes and cell manipulation as well as for an automated cell differentiation and feedback systems for growing processes in tissue engineering.For such applications of holographic interferometric metrology, the specific requirements for the analysis of cellular tissues and living cells have to be considered and a lateral resolution in microscop...

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Holography in Otology

Cited by 10 publications

References 10 publications

Single-shot, dual-mode, water-immersion microscopy platform for biological applications

Single-shot, dual-mode, water-immersion microscopy platform for biological applications

Spatially multiplexed interferometric microscopy with partially coherent illumination

Holographic interferometric microscopy systems for the application on biological samples

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