Holographic methods of three-dimensional visualization of microscopic phase objects

Titar

2008 4th International Conference on Advanced Optoelectronics and Lasers

et al. 2008

Self Cite

Blood erythrocytes morphology of pregnant women without hematological pathology, suffering from carbohydrates metabolic derangement, and newborn infants were investigated. Investigations were carried out using the digital holographic interference microscope. It has been found that erythrocytes morphology of the majority of the women did not correspond to the medical norm. Specific changes of erythrocytes morphology of patients with diabetes mellitus have not been detected. Morphological features of newborn infant erythrocytes have been visualized. Correlation of mother and child erythrocytes shapes has not been observed. The investigations have confirmed that erythrocytes underwent morphology changes not only in hematological diseases, but in pathology of different geneses. So, erythrocytes morphology reflects a state of an organism as a whole.

“…Correlation between erythrocyte shape of a mother and a child has not been detected. Fig.1 The fragment of the blood specimen of a new born infant [2].…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Using the digital holographic interference microscope for investigation of blood erythrocytes morphology of pregnant women and newborn infants

Titar

2008 4th International Conference on Advanced Optoelectronics and Lasers

et al. 2008

Self Cite

“…They are the Zernike phase-contrast method and the interference contrast method. These two contrast methods allow to University, Ukraine [1]. The first 3D images of blood erythrocytes were obtained using the microscope in [2].…”

Section: Holography Opens Up New Possibilitiesmentioning

confidence: 99%

Holographic Method for Blood Cell Imaging

Tishko¹,

Tishko²,

Titar³

2009

I&M

Human blood cells (erythrocytes) were imaged by the digital holographic interference microscope (DHIM) without any preparation. DHIM represents an effective technique for 3D imaging of phase microscopic objects and measurement of their morphological parameters. It has been detected that, in addition to hematological diseases, the diseases of different genesis and external factors serve as the reason for the morphological modification of blood erythrocytes.transform phase information into intensity modulation or into curvature of interference fringes, which can be detected by eye or photosensitive media. Since the problem of the 3-D visualization of phase microobjects has not been solved in classical microscopy, 3-D imaging of phase microobjects have been limited to electron microscopy. Unfortunately, this method is "destructive". Holography opened up new possibilities in microscopy of phase microobjects and resulted in the appearance of holographic analogs of the classical methods, which have some advantages. A combination of holographic microscopy and the methods for digital image processing has led to the new stage in microscopy. This makes it possible to realize the 3D visualization of phase microobjects. The first digital holographic interference microscope (DHIM), which allows the real-time 3D imaging of phase microobjects and the quantitative measurements of their parameters, has been created at the Laboratory of Holography, Kharkov National Holography Opens up New PossibilitiesCells of a living organism are phase microobjects. Such microobjects almost do not change the intensity of radiation transmitted through them, and cannot be observed using the methods of classical microscopy. Special methods are used in classical microscopy for noninvasive visualization of phase microobjects. They are the Zernike phase-contrast method and the interference contrast method. These two contrast methods allow to

“…The necessary period of the background interference fringes can be obtained by shifting the hologram from its original position. So, one can optically realize the holographic phase contrast method or the interference contrast method [7]. Interferograms of the MOs are recorded by means of an image-recording unit 14 (CCD) and transferred to a computer, where digital algorithms are used for their processing and MOs 3-D imaging.…”

Section: Experimental and Theoretical Principlesmentioning

confidence: 99%

“…If MOs have homogenous distribution of the refractive index, then deviations of the interference fringes from their initial directions on their interferogram are proportional to thicknesses of the MOs in the corresponding points [7]. The thickness of a MO at the point (x, y) can be expressed as:…”

Section: Experimental and Theoretical Principlesmentioning

confidence: 99%

<title>Erythrocytes three-dimensional morphology by digital holographic interference microscopy</title>

2008

Self Cite

We have developed the digital holographic interference microscope (DHIM) for real time three -dimensional (3-D) imaging and quantitative study of phase microscopic objects (MOs). The instrument integrates holographic interference microscope with digital processing of interferograms. The experimental procedure, allowing calculating microscopic objects thickness and 3-D imaging from the measured phase shift, is presented. The method has been applied to study 3-D morphology of red blood cells in norm, in pathology and under influence of physical and chemical factors in vivo. It has been found that morphologic changes of erythrocyte population had nonspecific character. Our investigations have proved that erythrocytes 3-D morphology reflects the state of a living organism and the level of its biological response on different external factors influence.