Abstract:We have developed the digital holographic interference microscope (DHIM) for real time three -dimensional (3-D) imaging and quantitative study of phase microscopic objects (MOs). The instrument integrates holographic interference microscope with digital processing of interferograms. The experimental procedure, allowing calculating microscopic objects thickness and 3-D imaging from the measured phase shift, is presented. The method has been applied to study 3-D morphology of red blood cells in norm, in patholog… Show more
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