Hollow-Fiber Flow Field-Flow Fractionation: A Pipeline to Scale Down Separation and Enhance Detection of Proteins and Cells

Membranes for separation of biomacromolecules and bioparticles via flow field-flow fractionation

“…Various ultrafiltration and microfiltration membranes have been used in FlFFF channels . Besides flat membranes, hollow fiber membranes have been also used, however, this mini‐review focuses on flat‐type channels.…”

Perspectives on protein biopolymers: miniaturized flow field-flow fractionation-assisted characterization of a single-cysteine mutated phaseolin expressed in transplastomic tobacco plants

“…Lastly, disposable usage of the separating channel eliminates sample carry-over or sample contamination issues [32] [33] [34] [35]. HF5 has proved its potential in the characterization of complex protein samples, monitoring of protein drug aggregation, and even monitoring of time-dependent formation of amyloid fibrils [24] [36] [37,38], but had not breached into the field of plant/food chemistry up to now. When online coupled to uncorrelated detection methods such as MALS and spectrophotometric detectors, HF5 provides high-resolution size distribution analysis.…”

A new approach for the separation, characterization and testing of potential prionoid protein aggregates through hollow-fiber flow field-flow fractionation and multi-angle light scattering

“…The cross flow represents the field needed for the separation and it can be adjusted in order to optimize separation process. Collectable fractions of size-homogeneous samples can therefore be obtained and used for selective on-line, off-line and activity tests [18][19][20]; disposable usage of the separating channel eliminates sample carry-over or sample contamination issues [18,19,21,22].…”