Membranes for separation of biomacromolecules and bioparticles via flow field-flow fractionation

Kavurt, U.B.; Marioli, Maria; Kok, Wim Th.; Stamatialis, Dimitrios

doi:10.1002/jctb.4473

Cited by 22 publications

(13 citation statements)

References 56 publications

(86 reference statements)

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“…The RC membrane with a 10 kDa MWCO is most commonly used for analysis of biological macromolecules [44,45]. Previous comparison of RC (10 kDa MWCO), triacetate cellulose, and polyethersulphone membranes showed the highest recovery of negatively charged VLPs with RC [46].…”

Section: Ultrafiltration Membrane-virus Interactionmentioning

confidence: 99%

Asymmetric flow field flow fractionation methods for virus purification

Eskelin

Lampi

Meier

et al. 2016

Journal of Chromatography A

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Detailed biochemical and biophysical characterization of viruses requires viral preparations of high quantity and purity. The optimization of virus production and purification is an essential, but laborious and time-consuming process. Asymmetric flow field flow fractionation (AF4) is an attractive alternative method for virus purification because it is a rapid and gentle separation method that should preserve viral infectivity. Here we optimized the AF4 conditions to be used for purification of a model virus, bacteriophage PRD1, from various types of starting materials. Our results show that AF4 is well suited for PRD1 purification as monitored by virus recovery and specific infectivity. Short analysis time and high sample loads enabled us to use AF4 for preparative scale purification of PRD1. Furthermore, we show that AF4 enables the rapid real-time analysis of progeny virus production in infected cells.

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Section: Ultrafiltration Membrane-virus Interactionmentioning

confidence: 99%

Asymmetric flow field flow fractionation methods for virus purification

Eskelin

Lampi

Meier

et al. 2016

Journal of Chromatography A

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“…Increasing the MWCO led to decreasing the void peaks, suggesting that membrane with larger MWCO performed better matrix removal efficiency (Kavurt et al 2015). The void peak observed when using 1 kDa RC with 0.02% FL-70 with 0.02% NaN 3 carrier was very high as compared to the other two membranes.…”

Section: Effect Of Membranes and Carrier Solutions On The Separationmentioning

confidence: 88%

Investigation of tin adsorption on silica nanoparticles by using flow field-flow fractionation with offline inductively coupled plasma mass spectrometry

Zulfah

Siripinyanond

2018

J Anal Sci Technol

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Flow field-flow fractionation (Fl-FFF) for silica nanoparticles with offline inductively coupled plasma mass spectrometry (ICP-MS) was applied to investigate the adsorption behavior of tin onto silica nanoparticles. Effect of carrier solutions and membranes was studied to achieve better separation for silica nanoparticles prior to tin detection using ICP-MS. Investigation was carried out by using 0.25 mM ammonium carbonate and 0.02% FL-70 with 0.02% NaN 3 as carrier solutions with 1 kDa regenerated cellulose (RC), 10 kDa regenerated cellulose (RC), and 10 kDa polyethersulfone (PES) membranes. Ammonium carbonate carrier solution with suitable ionic strength provided good separation with minimization of particle-membrane interaction. Better retention was shown by employing 10 kDa RC membrane. Furthermore, Fl-FFF was employed for the separation of silica nanoparticles incubated with tin. Fractions eluted from Fl-FFF were collected and then introduced into ICP-MS. Tin was adsorbed onto silica nanoparticles with different adsorption capabilities depending on particle size. Adsorption of tin was greater on the smaller size of silica nanoparticles compared to the bigger size with the adsorption percentage of 98.5, 44.9, and 6.5 for 60 nm, 100 nm, and 200 nm, respectively. Size-dependent adsorption of tin was in good agreement with surface area per volume of silica nanoparticles.

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“…The FlFFF system in previous studies was usually equipped with membranes with varied materials and MW cut‐off (MWCO) (Kavurt et al 2015). Fractionation results using polyethersulfone membrane with MWCO of 0.3 and 1 kDa were thus compared.…”

Section: Assessment and Discussionmentioning

confidence: 99%

“…In addition, the SEC exhibited shear force and damage to the sample particles. Flow field‐flow fractionation (FlFFF), however, is a novel technique to separate the complex materials via filed‐driven force and liquid stream, which enables it suitable for the fractionation of macromolecules, colloids, and particles (Unrine et al 2012; Hinterwirth et al 2013; Kavurt et al 2015). Compared to current techniques, FlFFF exhibited evident advantages in terms of the wide fractionation range, mild fractionation conditions, and minor adsorption and shear effects on samples.…”

mentioning

confidence: 99%

Characterization and modification of the molecular weight distribution within dissolved organic matter using flow field‐flow fractionation

Pan

2020

Limnology & Ocean Methods

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Dissolved organic matter (DOM) exists ubiquitously in the aquatic environment with varying chemical compositions and a wide molecular weight (MW) distribution, which, as a result, exhibits differing impacts on the behavior and fate of contaminants. The current methods for the MW characterization of DOM usually possess some weaknesses and therefore, some novel techniques are needed. In this study, the feasibility and sensitivity of flow field-flow fractionation (FlFFF) system were studied for the MW characterization of DOMs from various origins. The systematic evaluation of FlFFF indicated that a mobile phase consisting of 10 mM NaCl and 5 mM H 3 BO 3 and with a pH of 8 was optimal, and the fractionation program with a cross-flow rate of 2 mL/min and a focusing time of 2 min resulted in the most efficient MW fractionation. Compared to natural waters, the standard DOM materials possessed a high proportion of humic-like substances and narrow MW distribution. The quantification analysis showed that chromophoric organic ligands were mainly distributed in the high (i.e., 100 kDa-0.45 μm) and low (i.e., 0.3-1 kDa) MW fractions for natural waters, but in the medium (i.e., 1-100 kDa) and low MW fractions for the standard DOM materials. However, the fluorescent humic-like substances were predominantly distributed within the medium and low MW fractions, irrespective of DOM types. This study showed the feasibility and sensibility of FlFFF in fractionating DOM samples, and can be used to quantify the different MW distribution of chromophoric and fluorescent components in standard and natural DOM.

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Membranes for separation of biomacromolecules and bioparticles via flow field-flow fractionation

Cited by 22 publications

References 56 publications

Asymmetric flow field flow fractionation methods for virus purification

Asymmetric flow field flow fractionation methods for virus purification

Investigation of tin adsorption on silica nanoparticles by using flow field-flow fractionation with offline inductively coupled plasma mass spectrometry

Characterization and modification of the molecular weight distribution within dissolved organic matter using flow field‐flow fractionation

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