Hofmeister effects on the colloidal stability of an IgG-coated polystyrene latex

López-León, Teresa; Jódar-Reyes, Ana Belén; Ortega-Vinuesa, J.L.; Bastos‐González, Delfi

doi:10.1016/j.jcis.2004.10.021

Cited by 53 publications

(71 citation statements)

References 46 publications

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“…Similar behaviour of polyelectrolyte-coated particles was previously reported in the presence of monovalent anionso fh igh concentration. [40,56] In addition, ion condensation and subsequentc hange in the effective chargew as previously reported with synthetic and natural polyelectrolytes. [49] Such ap henomenonm ay lead to slight charge reversal of the polyelectrolyte-coated colloidal particles at high electrolyte concentrations.…”

Section: Resultsmentioning

confidence: 72%

“…It is suspected that this charge reversal is due to the specific affinity of the chloride anions to the enzyme, and this adsorption process turns the surface charge of TNS–PDADMAC–SOD negative at elevated salt levels. Similar behaviour of polyelectrolyte‐coated particles was previously reported in the presence of monovalent anions of high concentration . In addition, ion condensation and subsequent change in the effective charge was previously reported with synthetic and natural polyelectrolytes .…”

Section: Resultsmentioning

confidence: 99%

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Immobilization of Superoxide Dismutase on Polyelectrolyte‐Functionalized Titania Nanosheets

2017

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The superoxide dismutase (SOD) enzyme was successfully immobilized on titania nanosheets (TNS) functionalized with the poly(diallyldimethylammonium chloride) (PDADMAC) polyelectrolyte. The TNS-PDADMAC solid support was prepared by hydrothermal synthesis followed by self-assembled polyelectrolyte layer formation. It was found that SOD strongly adsorbed onto oppositely charged TNS-PDADMAC through electrostatic and hydrophobic interactions. The TNS-PDADMAC-SOD material was characterized by light scattering and microscopy techniques. Colloidal stability studies revealed that the obtained nanocomposites possessed good resistance against salt-induced aggregation in aqueous suspensions. The enzyme kept its functional integrity upon immobilization; therefore, TNS-PDADMAC-SOD showed excellent superoxide radical anion scavenging activity. The developed system is a promising candidate for applications in which suspensions of antioxidant activity are required in the manufacturing processes.

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Section: Resultsmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Immobilization of Superoxide Dismutase on Polyelectrolyte‐Functionalized Titania Nanosheets

2017

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“…[153][154][155][156][157][158][159][160][161] Two major findings have emerged from all these investigations. Contrary to what was expected for chargestabilized particles from traditional DLVO theory, which foresees a progressive destabilization of particles as the ionic strength increases, protein coated particles show a restabilization at high ionic strengths.…”

Section: Fundamental Interactions Between Proteins and Surfacesmentioning

confidence: 98%

Nanoparticle colloidal stability in cell culture media and impact on cellular interactions

et al. 2015

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Nanomaterials are finding increasing use for biomedical applications such as imaging, diagnostics, and drug delivery. While it is well understood that nanoparticle (NP) physico-chemical properties can dictate biological responses and interactions, it has been difficult to outline a unifying framework to directly link NP properties to expected in vitro and in vivo outcomes. When introduced to complex biological media containing electrolytes, proteins, lipids, etc., nanoparticles (NPs) are subjected to a range of forces which determine their behavior in this environment. One aspect of NP behavior in biological systems that is often understated or overlooked is aggregation. NP aggregation will significantly alter in vitro behavior (dosimetry, NP uptake, cytotoxicity), as well as in vivo fate (pharmacokinetics, toxicity, biodistribution). Thus, understanding the factors driving NP colloidal stability and aggregation is paramount. Furthermore, studying biological interactions with NPs at the nanoscale level requires an interdisciplinary effort with a robust understanding of multiple characterization techniques. This review examines the factors that determine NP colloidal stability, the various efforts to stabilize NP in biological media, the methods to characterize NP colloidal stability in situ, and provides a discussion regarding NP interactions with cells.

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“…For example, measured electrophoretic mobilities of latex particles showed ion specificity. With the same pH value and bulk concentration, the mobilities of particles suspended into different 1-1 symmetric electrolytes are different (Gallardo et al 1993; Lopez-Leon et al 2003; 2005; Salis et al 2012). It was also observed that the dependence of the mobility on salts follows certain order (Lopez-Leon et al 2003; 2005).…”

Section: Introductionmentioning

confidence: 99%

The influence of dielectric decrement on electrokinetics

Zhao

Zhai

2013

J. Fluid Mech.

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We treat the dielectric decrement induced by excess ion polarization as a source of ion specificity and explore its impact on electrokinetics. We employ a modified Poisson-Nernst-Planck (PNP) equations accounting for the dielectric decrement. The dielectric decrement is determined by the excess ion polarization parameter α and when α = 0 the standard PNP model is recovered. Our model shows that ions saturate at large zeta potentials (ζ). Because of ion saturation, a condensed counterion layer forms adjacent to the charged surface, introducing a new length scale, the thickness of the condensed layer (lc). For the electro-osmotic mobility, the dielectric decrement weakens the electro-osmotic flow owing to the decrease of the dielectric permittivity. At large ζ, when α ≠ 0, the electro-osmotic mobility is found to be proportional to ζ/2, in contrast to ζ predicted by the standard PNP model. This is attributed to ion saturation at large ζ. In terms of the electrophoretic mobility Me, we carry out both an asymptotic analysis in the thin-double-layer limit and solve the full modified PNP model to compute Me. Our analysis reveals that the impact of the dielectric decrement is intriguing. At small and moderate ζ, the dielectric decrement decreases Me with an increasing α. At large ζ, it is well known that the surface conduction becomes significant and plays an important role in determining Me. It is observed that the dielectric decrement effectively reduces the surface conduction. Hence in stark contrast, Me increases as α increases. Our predictions of the contrast dependence of the mobility on α at different zeta potentials qualitatively agree with experimental results on the dependence of the mobility among ions and provide a possible explanation for such ion specificity. Finally, the comparisons between the thin-double-layer asymptotic analysis and the full simulations of the modified PNP model suggest that at large ζ the validity of the thin-double-layer approximation is determined by lc rather than the traditional Debye length.

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Hofmeister effects on the colloidal stability of an IgG-coated polystyrene latex

Cited by 53 publications

References 46 publications

Immobilization of Superoxide Dismutase on Polyelectrolyte‐Functionalized Titania Nanosheets

Immobilization of Superoxide Dismutase on Polyelectrolyte‐Functionalized Titania Nanosheets

Nanoparticle colloidal stability in cell culture media and impact on cellular interactions

The influence of dielectric decrement on electrokinetics

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