Hochdruckflüssigkeits-chromatographische Trennung und fluorimetrische Bestimmung primärer α,ω-Diamine nach der Derivatisierung zu α,ω-Bis(2,2-diphenyl-1-oxa-3-azonia-2-borata-3-naphthyl)alkanen

Claas, K. E.; Hohaus, E.; Monien, H.

doi:10.1007/bf00497476

Cited by 8 publications

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“…Amines can also be separated and quantitatively determined in the form of their acetyl derivates, using a mixture of ethanol, water and methylenechloride as eluent on a Spherosil XOA 400 column [3]. The derivatization by the reagent DOOB to form fluorescent azomethine chelates is a selective method for primary amines [4,5]. The advantage of this method is that it is specific for primary amines and does not form by-products with phenols or thiols.…”

Section: Introductionmentioning

confidence: 99%

High-performance liquid chromatographic determination of primary amines in aqueous solutions after extraction and derivatization with 2,2-diphenyl-1-oxa-3-oxonia-2-boratanaphthalene (DOOB)

Sánchez-Rodas

Hohaus

Wenclawiak

1996

Analytical and Bioanalytical Chemistry

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Aqueous solutions of tryptamine, tyramine and ethanolamine are extracted and derivatizated to azomethine chelates using a solution of DOOB in diethyl ether. The extraction takes about 2 h. Diisopropyl ether and toluol have also been tested as extractants. The separation of the azomethine chelates is realized by a gradient program on a Si 60 column using a mixture of n-heptane/tert-butyl methyl ether. UV detection is carried out at 280 nm. The concentration of these three amines has been determined in a Chianti wine sample.

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Section: Introductionmentioning

confidence: 99%