Hochdruckflüssig-chromatographische Bestimmung von Biotin in Arzneimitteln

Röder, Erhard; Engelbert, U.; Troschütz, Jutta

doi:10.1007/bf00466251

Cited by 17 publications

(3 citation statements)

References 16 publications

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“…Besides, UV detection in the HPLC method is not suitable for biotin detection because its maximum absorption wavelength is at 210 nm, which is similar to that of most organic solvents. The method we used in this study has LODs of 50-100 ng and a recovery rate of 99.6-99.7%, which all fall in the range of that of HPLC (1-1000 ng for LODs and 89.3-104.7% for recovery rate) [18][19][20][21][22][23][24][25][26][27][28][29]. Our present method was proved to be an efficient, simple, and precise method for the quantitative analysis of biotin.…”

Section: Hptlc and Total Biotin Contentmentioning

confidence: 72%

“…The method is also rapid because the developing time is about 35 min but at least nine samples could be detected simultaneously on one single plate. The retention time of HPLC is 5–26 min . The HPTLC method is time saving.…”

Section: Resultsmentioning

confidence: 99%

“…Analytical techniques for biotin determination include microbiological assays , spectrophotometric methods , CZE , HPLC , LC–MS , TLC , over‐pressure layer chromatography , and binding assays . The HPLC detectors include UV, fluorescence, and electrochemical detectors.…”

Section: Introductionmentioning

confidence: 99%

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Determination of biotin in Antarctic krill (Euphausia superba) by high‐performance TLC with different post‐chromatographic derivatizations

Teo

Liu

2013

J of Separation Science

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A new efficient method was developed to detect biotin in Antarctic krill by Vis-absorbance detection. DMF was used after chloroform pretreatment to extract biotin and two chromogenic methods were developed. The development system consisted of dichloromethane/dimethylcarbinol/methanol/glacial acetic acid (3:3:2:0.015, v/v/v/v). Samples were separated on precoated silica gel GF254 high-performance TLC plates. Densitometric analysis of biotin was carried out in the absorbance mode at 400 and 530 nm. The biotin content was determined to be 1.0948 ± 0.0097 and 1.1212 ± 0.0155 mg/g in Antarctic krill with the two chromogenic methods, which had no significant difference.

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