HMQ‐T‐F2 exert antitumour effects by upregulation of Axin in human cervical HeLa cells

Abstract

Looking for novel, effective and less toxic therapies for cervical cancer is of significant importance. In this study, we reported that HMQ‐T‐F2(F2) significantly inhibited cell proliferation and transplantable tumour growth. Mechanistically, HMQ‐T‐F2 inhibited HeLa cell growth through repressing the expression and nuclear translocation of β‐catenin, enhancing Axin expression, as well as downregulating the Wnt downstream targeted proteins. Knock‐down of a checkpoint β‐catenin by siRNA significantly attenuated HeLa cell proliferation. Furthermore, XAV939, an inhibitor of β‐catenin, was used to treat HeLa cells and the results demonstrated that HMQ‐T‐F2 inhibited proliferation and migration via the inhibition of the Wnt/β‐catenin pathway.

HMQ‑T‑F2 suppresses migration of the human cervical cancer HeLa cells by reversing EMT via the PI3K/Akt signaling pathway

“…Our previous study demonstrated that β-catenin is an important target of F2, and it was confirmed that F2 regulates the β-catenin destruction complex that consists of Axin, APC, and CK1 (20). It is well known that β-catenin is a key player in abnormal Wnt signaling and is the most important oncogene in cervical cancer biology due to its ability to regulate cell migration.…”

“…The ability of F2 to inhibit migration was evaluated using wound scratch and Transwell migration assays. Our previous study concluded that treatment of F2 at a dosage of 0.6 µmol/l for 48 h had no cytotoxic effect on HeLa cells (20). In addition, an MTT assay revealed that F2 had no inhibition on human normal cervical epithelial cells even at 25 µmol/l, in the previous study.…”

“…Development of novel natural therapeutic reagents for cervical cancer is a fast-growing field of research. According to our previous study, HMQ-T-F2, a taspine derivative, effectively inhibited cervical HeLa cell proliferation in vitro and in vivo by upregulating Axin and suppressing nuclear translocation of β-catenin (20). However, little is known about the effect of HMQ-T-F2 on HeLa cell migration.…”

<em>SMAGP</em> a novel biomarker of cervical cancer development and progression

“…However, treatment for advanced stage cervical cancer remains a public health concern. 4 , 5 In addition, the prognosis of patients with cervical cancer is influenced by numerous factors and not all women with this disease fully recover. Although research into cervical cancer has recently progressed significantly, the condition remains associated with high levels of morbidity and mortality, partly due to a lack of clarity regarding its underlying molecular mechanisms.…”

Synergistic effect of berberine and HMQ1611 impairs cell proliferation and migration by regulating Wnt signaling pathway in hepatocellular carcinoma

“…Wnt/β‐catenin signaling is involved in a multitude of developmental processes and the maintenance of adult tissue homeostasis by regulating cell proliferation, differentiation, migration, and apoptosis and by maintaining adult stem cells in a pluripotent state (Dai et al, ). Not surprisingly, aberrant regulation of this pathway is therefore associated with a variety of diseases, including cancer and neurodegeneration (Clevers, ; Rhee et al, ).…”