HLA-DRw6 and Its Complexity

The production of a human monoclonal antibody defining a split of HLA‐DRw13 (DRw13b)

“…The reagent also recognized a subtype of HLA-DRwl3 associated with DQw7. NDS40 does not react with the DRw8X14 antigen (8) or the DQw7-linked 'COR antigen (9,10).…”

HLA class II specificities and haplotypes in South Africa detected using polymerase chain reaction and sequence‐specific oligonucleotide typing

“…During the Tenth Workshop, the existence of a new DR specificity was proposed on the basis of serological and Southern blot studies, and was given the name DRwl2 x 6 on the basis of positive reactions with DRwl2 sera plus some DRw6 sera (2). This specificity was seen in linkage disequilibrium with DQwS and DQw7, and was observed to be homozygous in two families.…”

“…This new DR specificity was proposed during the Tenth Workshop on the basis of equally good reac-tivitywithbothDRw8 and DRwl4sera (2). We have studied the segregation of this haplotype in two families of mixed ancestry using PCR and the 11WS oligonucleotides, and the results are shown in Table 3.…”

“…Several methods, including serology, cellular typing and RFLPs, have been used to identify the occurrence of new HLA class I1 specificities in the populations of South Africa (1)(2)(3)(4)(5)(6). Recently, the use of the polymerase chain reaction with sequence-specific oligonucleotide typing (7) makes it possible to identify a new allele at the nucleotide sequence level by means of a unique pattern of hybridization using several probes.…”