HLA‐A, HLA‐B, and HLA‐DRB1 alleles and haplotypes in Naxi and Han populations in southwestern China (Yunnan province)

Abstract

The frequencies of the human leukocyte antigen alleles HLA-A, HLA-B, and HLA-DRB1 and the A-B-DRB1, A-B, and B-DRB1 haplotypes were studied in Naxi and Yunnan Han populations using polymerase chain reaction (PCR)-sequence-specific amplification for alleles A and B and a PCR-microtiter plate hybridization method for the DRB1 allele. A total of 8 A, 19 B, and 30 DRB1 alleles were found in the Naxi population, and 15 A, 21 B, and 36 DRB1 alleles were found in Yunnan Han population. The common A-B-DRB1 haplotypes in the Naxi population were A*24-B*15-DRB1*1202, A*11-B*15-DRB1*0405, A*11-B*15-DRB1*1202, A*11-B*38-DRB1*08032, and A*11-B*55-DRB1*0405; the common A-B haplotypes were A*11-B*15, A*11-B*38, and A*24-B*15; and the common B-DRB1 haplotypes were B*15-DRB1*1202, B*38-DRB1*08032, and B*48-DRB1*1201. In the Yunnan Han population, the common A-B-DRB1 haplotypes were A*24-B*15-DRB1*1501, A*24-B*46-DRB1*08032, and A*24-B*15-DRB1*1201; the common A-B haplotypes were A*24-B*15, A*24-B*46, and A*34-B*46; and the common B-DRB1 haplotypes were B*15-DRB1*1501, B*46-DRB1*09012, and B*46-DRB1*1401. Phylogenetic tree and principal component analyzes based on HLA-A, HLA-B, and DRB1 allele frequencies suggested that the Naxi ethnic group belongs to the southern Chinese groups, while the Yunnan Han population is a characteristic population located intermediate between northern and southern Chinese groups, although they live in the southwest of China.

Associations of killer cell immunoglobulin-like receptor genes with complications of rheumatoid arthritis

“…[25][26][27][28][29] Therefore, gene-disease associations in Caucasians and Chinese may be different, as has already been described in several other diseases. …”

HLA polymorphism of the Zhuang population reflects the common HLA characteristics among Zhuang-Dong language-speaking populations

“…Our previous studies based on HLA distributions suggested that the same linguistic-speaking populations were genetically similar to each other (Shi et al, 2006;Ogata et al, 2007). The other studies of Y chromosomes, mitochondrial DNA, as well as short tandem repeat, have also demonstrated that the Zhuang-Dong language-speaking population showed close affinity (Yao et al, 2002;Shi et al, 2005;Tian et al, 2008).…”

“…Our previous studies based on HLA polymorphisms in several ethnic groups of Southwest China have suggested that the same linguistic populations tend to cluster together (Shi et al, 2006;Ogata et al, 2007). As a typical Zhuang-Dong languagespeaking population, the analyses of HLA genes and haplotypes in the Zhuang population would be helpful for indentifying the common HLA characteristics of the same linguistic family.…”

Distributions of human leukocyte antigen–A, –B, and –DRB1 alleles and haplotypes based on 46,915 Taiwanese donors

“…Comparison of populations was limited by the availability of allele-level data for HLA-A, -B, and -DRB1 loci. We chose 10 populations, including Korean [18], Western Javanese [20], Japanese [22], Vietnamese [23], Maonan in China [24], Southern Chinese [25,26], Northern Chinese [27], North African immigrants in France, French, and Eastern European Americans [28] for the reason that their donors were HLA typed with the same level of resolution in the HLA-A, -B, and -DRB1 loci as our donors. We performed the PCA and clustering analysis based on 21 commonly shared HLA-A, -B, and -DRB1 alleles and their corresponding frequencies for the two Taiwanese groups and the 10 chosen worldwide populations.…”