HLA‐A, ‐B, ‐C, ‐DRB1 and ‐DQB1 alleles and haplotypes in the Kinh population in Vietnam

Abstract

Allele and haplotype frequencies of the human leukocyte antigens (HLA) were studied in the Kinh Vietnamese population. We analyzed 170 unrelated healthy individuals. DNA-based HLA typing was performed using a microsphere-based array genotyping platform with sequence-specific oligonucleotide probes to distinguish HLA-A, -B, -C, -DRB1 and -DQB1 alleles. A total of 21 HLA-A, 37 HLA-B, 18 HLA-C, 25 HLA-DRB1, and 14 HLA-DQB1 alleles were identified. HLA-A*1101, A*2402, A*3303, B*1502, B*4601, Cw*0102, Cw*0702, Cw*0801, DRB1*1202, DQB1*0301, DQB1*0303, and DQB1*0501 were found with frequencies higher than 10%. Two representative haplotypes bearing two to five HLA loci were A*1101-B*1502 and A*3303-B*5801 for HLA-A-B; Cw*0801-B*1502 and Cw*0102-B*4601 for HLA-C-B; B*1502-DRB1*1202 and B*4601-DRB1*0901 for HLA-B-DRB1; DRB1*1202-DQB1*0301 and DRB1*0901-DQB1*0303 for HLA-DRB1-DQB1; A*1101-Cw*0801-B*1502 and A*3303-Cw*0302-B*5801 for HLA-A-C-B; A*1101-B*1502-DRB1*1202 and A*2901-B*0705-DRB1*1001 for HLA-A-B-DRB1, A*1101-Cw*0801-B*1502-DRB1*1202-DQB1*0301 and A*2901-Cw*1505-B*0705-DRB1*1001-DQB1*0501 for HLA-A-C-B-DRB1-DQB1. Allele distribution and haplotype analysis demonstrated that the Vietnamese population shares HLA patterns with southern Chinese, Thai, Javanese and Micronesians, while it also retains unique characteristics.

HLA polymorphism of the Zhuang population reflects the common HLA characteristics among Zhuang-Dong language-speaking populations

“…For example, the predominant A-C-B haplotype, A*02:07-C*01:02-B*46:01, was predominant in Vietnamese as well as in some southern Chinese populations, such as the Dai, Hani, Miao, Bouyei, Shui, Han in Yunnan, and Han in Guangdong, but existed at low frequencies in Chinese Hui, Japanese, and Koreans. Another predominant haplotype, A*11:01-C*08:01-B*15:02, was also predominant in Vietnamese and some Chinese populations, such as the Bulang, Dai, Hani, Shui, Han in Yunnan, and Han in Guangdong, who were located in southern China (Tokunaga et al, 1997;Middleton et al, 2004;Chen et al, 2007;Hong et al, 2007;Ogata et al, 2007;Trachtenberg et al, 2007;Hoa et al, 2008;Shi et al, 2008;2010a;2010b;Wen et al, 2008;Yao et al, 2009). Based on the HLA alleles, haplotypes, and neighbor-joining tree analysis, we concluded that the Zhuang is a typical southern Chinese population.…”

“…For example, A*11:01-B*15:02-DRB1*12:02 was common in southern populations of East Asia (Ogata et al, 2007;Trachtenberg et al, 2007;Hoa et al, 2008;Shi et al, 2008;2010a;2010b;Wen et al, 2008;Gendzekhadze et al, 2009;Yao et al, 2009); however, it was not identified in the Zhuang. Instead, A*11:01-B*15:02-DRB1*15:01 was predominant in the Zhuang, but was not identified in other East Asian populations.…”

Distributions of human leukocyte antigen–A, –B, and –DRB1 alleles and haplotypes based on 46,915 Taiwanese donors

“…Comparison of populations was limited by the availability of allele-level data for HLA-A, -B, and -DRB1 loci. We chose 10 populations, including Korean [18], Western Javanese [20], Japanese [22], Vietnamese [23], Maonan in China [24], Southern Chinese [25,26], Northern Chinese [27], North African immigrants in France, French, and Eastern European Americans [28] for the reason that their donors were HLA typed with the same level of resolution in the HLA-A, -B, and -DRB1 loci as our donors. We performed the PCA and clustering analysis based on 21 commonly shared HLA-A, -B, and -DRB1 alleles and their corresponding frequencies for the two Taiwanese groups and the 10 chosen worldwide populations.…”

Nasopharyngeal Carcinoma (NPC) Related Human Leukocyte Antigen (HLA) Haplotype Sharing among Southern East Asian Population

“…18,20 This haplotype is commonly found among general population. With those regard, the estimated frequencies of this haplotype in different populations using the maximum likelihood method are reliable for presuming the susceptibility to the disease in each population in Southern East Asia.…”

“…The HLA allele frequency at four digit level for HLA-A, -B and -DRB1 were obtained from nineteen populations both from previous studies {Javanese (Indonesia), 7 Kihn (Vietnamese), 20 Han (Yunnan, China), 21 Maonan (Guangxi, China), 22 Dai (Yunnan, China), 23 Hani (China), 24 Bulang (China), 24 Jinuo (China), 25 and Wa (China) 25 } and from www.allelefrequencies.net {Malay, Han (Singapore), Thai, Han (Yunnan, China), Jinuo (China), Wa (Yunnan, China), Han (Hong Kong), Han (Guangdong, China), Taiwanese, Han (Fengyuandong, China), Maonan (Guangxi, China), Buyi (China), Nu (China), and Lisu (Yunnan, China)}. Only populations with minimum sample size of 100 individuals were selected (Fig.…”