Background Human Immune Virus’ positive status non-disclosure to a sexual partner is a public health problem where a high prevalence of Human Immune Virus infection is reported on a region with poor partner disclosure status. The aim of this study was to assess Human Immune Virus positive status disclosure to sexual partners and associated factors among adult Human Immune Virus positive clients at Debre Markos town, Amhara Regional State Ethiopia, in, 2019. Methods Facility based cross-sectional study was done among 421 adult Human Immune Virus positive clients in Debre Markos town from September 1-30-2019. Consecutive sampling was used to collect the data. Epidata version 3.1 for data entry and SPSS version 25 for data analysis were used. Hosmer-Lemeshow goodness test of model fitness was checked. Bivariable and multivariable logistic analysis (p<0.05) was performed. Results The median age of participants was 35 years with (IQ of 30-42). Human Immune Virus positive status disclosure was 73.4% (95%, CI: 69-78). Male Sex (AOR=3.04; 95%, CI: 1.75-5.27), urban residence (AOR=2.57; 95%, CI: 1.39-4.77), Good antiretroviral treatment adherence status (AOR=3.18; 95%, CI: 1.58-6.39), stayed 39 months and above on antiretroviral treatment (AOR=4.53; 95%, CI: 2.31-8.88), Knowing sexual partner`s Human Immune Virus status (AOR=2.74; 95%, CI: 1.63-4.63) and provider-initiated Human Immune Virus testing (AOR=2.18; 95%, CI: 1.21-3.92) were statistically significant associated factors with Human Immune Virus status positive status disclosure. Conclusions and recommendation Human Immune Virus’ positive status disclosure to sexual partner was low. Sex, Residence, Duration of Anti-Retroviral Treatment, Adherence status to antiretroviral treatment, provider-initiated Human Immune Virus testing and knowing sexual partner`s Human Immune Virus status have had a statistically significant association with disclosure. It is better to promote provider-initiated Human Immune Virus testing and give special attention to females and rural residents regarding Human Immune Virus status disclosure to sexual partners.